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Cyclocarya paliurus rooting culture medium and culture method thereof

The technology of a rooting medium and a cultivation method, which is applied in the field of Cyclocarya paliurus rooting medium and its cultivation, can solve the problems of low rooting rate, difficulty in meeting industrial seedling cultivation, and poor rooting quality, so as to achieve robust plant growth, promote rooting, good root development

Active Publication Date: 2016-11-16
GUILIN BOLIN BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing research results show that aseptic seedlings of Cyclocarya paliurus can be propagated successfully, but its rooting rate is low and rooting quality is poor, so it is difficult to meet the requirements of industrial seedling cultivation

Method used

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  • Cyclocarya paliurus rooting culture medium and culture method thereof
  • Cyclocarya paliurus rooting culture medium and culture method thereof
  • Cyclocarya paliurus rooting culture medium and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1) Get the current year's branches of Cyclocarya paliurus, and obtain test-tube seedlings by conventional method in vitro culture, such as figure 1 shown;

[0036]2) Rooting culture: cut out a single test-tube plantlet that is higher than 3.5 cm and has 3 to 4 compound leaves, insert it into the rooting medium and cultivate it for 30 days (when culturing for 15 days, if figure 2 As shown, in culture 30d as image 3 shown); where:

[0037] The formula of the rooting medium is: improved MS+IBA 1.0 mg / L+NAA 0.5 mg / L+ diethyl hexanoate 0.05 mg / L+ sucrose 20 g / L, pH 5.8, and a filter paper bridge is used as a support.

[0038] The culture conditions in the rooting medium are as follows: the temperature is 23-25°C, the light cycle is 12h / d, and the light intensity is 2000Lx.

[0039] Calculate the rooting rate (the same below) by the number of rooted strains / inoculated strains × 100%, and the rooting rate is 84%.

[0040] Further, the rooted seedlings obtained by cultiva...

Embodiment 2

[0051] 1) Get the current year's branches of Cyclocarya paliurus, and obtain test-tube seedlings by in vitro culture according to conventional methods;

[0052] 2) Rooting culture: cut out a single test-tube plantlet that is higher than 2.5 cm and has 2 to 3 compound leaves, and insert it into the rooting medium for 25 days; wherein:

[0053] The formula of the rooting medium is: improved MS+IBA0.5mg / L+NAA1.0mg / L+ diethylaminohexanoate 0.1mg / L+ sucrose 20g / L, pH6.0, using filter paper bridge as support.

[0054] The culture conditions in the rooting medium are as follows: the temperature is 25° C., the light cycle is 10 h / d, and the light intensity is 3000 Lx.

[0055] After calculation, the rooting rate is 70%.

Embodiment 3

[0057] 1) Get the current year's branches of Cyclocarya paliurus, and obtain test-tube seedlings by in vitro culture according to conventional methods;

[0058] 2) Rooting culture: select a single test-tube plantlet with a height greater than 3 cm and 2 to 3 compound leaves, cut off the callus at the base, and then insert it into the rooting medium and cultivate it for 28 days; wherein:

[0059] The formula of the rooting medium is: improved MS+IBA 2.0 mg / L+ diethyl hexanoate 0.08 mg / L+ sucrose 16 g / L, pH 6.0, using a filter paper bridge as a support;

[0060] The culture conditions in the rooting medium are as follows: the temperature is 27° C., the light cycle is 10 h / d, and the light intensity is 2800 Lx.

[0061] After calculation, the rooting rate is 78%.

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Abstract

The invention discloses a cyclocarya paliurus rooting culture medium and a culture method thereof. The rooting culture medium comprises improved MS, 0.5-2.0mg / L IBA, 0-1.0mg / L NAA, 0.02-0.1mg / L diethyl aminoethyl hexanoate and 15-20g / L sucrose, wherein the improved MS comprises macro-elements, an organic compound and trace elements identical with the MS culture medium in concentration; the macro-elements comprise 700mg / L potassium nitrate, 600mg / L ammonium nitrate, 185mg / L magnesium sulfate heptahydrate, 60mg / L potassium dihydrogen phosphate and 165mg / L calcium chloride dehydrate; the organic compound comprises 100mg / L inositol, 1mg / L hydrochloric acid, 1mg / L pyridoxine hydrochloride, 0.2mg / L thiamine hydrochloride and 2mg / L glycine. When the cyclocarya paliurus rooting culture medium is used for performing rooting culture on cyclocarya paliurus test-tube seedlings, the rooting rate is not smaller than 70%.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a rooting culture medium and a culture method of Cyclocarya paliurus. Background technique [0002] Cyclocarya paliurus (Batal.) Iljinskaja is a plant of the genus Cyclocarya paliurus (Batal.) Iljinskaja in the Juglandaceae family. Known as the "giant panda of plants" and "the third tree in the medical field", Cyclocarya paliurus is a rare tree species that survived the Quaternary Ice Age. It only exists in China and is an endangered plant under national key protection. Cyclocarya paliurus is mainly distributed in Jiangxi, Zhejiang, Jiangsu, Anhui, Fujian, Taiwan, Hubei, Sichuan, Guizhou, Yunnan and other places, mostly scattered in deep mountains and old forests and some nature reserves. [0003] Cyclocarya paliurus has unique health care and medicinal value. Folks have long used the green papaya leaves to make tea, the tea has a delicate fragrance and a sweet taste. "Chines...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 莫海萍苏玉卿李伯林刘云
Owner GUILIN BOLIN BIOLOGICAL TECH CO LTD