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A rooting medium for Cyclocarya test-tube plantlets and a method for tissue culture and rapid propagation of Cyclocarya

A rooting medium, tissue culture and rapid propagation technology, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of difficult to meet industrial seedling cultivation, low rooting rate, poor rooting quality, etc., to promote rooting , The effect of strong plant growth and good root development

Active Publication Date: 2018-05-11
GUILIN BOLIN BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing research results show that test-tube seedlings of Cyclocarya paliurus can successfully propagate, but its rooting rate is low and rooting quality is poor, so it is difficult to meet the requirements of industrial seedling cultivation

Method used

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  • A rooting medium for Cyclocarya test-tube plantlets and a method for tissue culture and rapid propagation of Cyclocarya
  • A rooting medium for Cyclocarya test-tube plantlets and a method for tissue culture and rapid propagation of Cyclocarya

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Get the current year's branches of Cyclocarya paliurus, and obtain test-tube seedlings by conventional method in vitro culture, such as figure 1 shown;

[0034] 2) Rooting culture: cut out a single test-tube plantlet that is higher than 3.5 cm and has 3 to 4 compound leaves, and insert it into the rooting medium for 35 days (the picture of the root of the rooted plantlet at the time of 35 days of culture is as follows: figure 2 shown); where:

[0035]The formula of the rooting medium is: improved MS+IBA0.5mg / L+IAA1.0mg / L+ diethyl hexanoate 0.05mg / L+ sucrose 20g / L+ agar 7g / L, pH5.8.

[0036] The rooting culture conditions are as follows: the temperature is 23-25°C, the light cycle is 12h / d, and the light intensity is 2000Lx.

[0037] Calculate the rooting rate (the same below) by the number of rooted strains / inoculated strains × 100%, and the rooting rate is 90%.

Embodiment 2

[0048] 1) Get the current year's branches of Cyclocarya paliurus, and obtain test-tube seedlings by in vitro culture according to conventional methods;

[0049] 2) Rooting culture: cut out a single test-tube plantlet that is higher than 3.5 cm and has 3 to 4 compound leaves, and insert it into the rooting medium for 25 days; wherein:

[0050] The formula of the rooting medium is: improved MS+IBA 1.0 mg / L+IAA 1.0 mg / L+ diethyl hexanoate 0.05 mg / L+ sucrose 18 g / L+ agar 7 g / L, pH 5.8.

[0051] The culture conditions in the rooting medium are as follows: the temperature is 23-25°C, the light cycle is 12h / d, and the light intensity is 2000Lx.

[0052] After calculation, the rooting rate is 84%.

Embodiment 3

[0054] 1) Get the current year's branches of Cyclocarya paliurus, and obtain test-tube seedlings by in vitro culture according to conventional methods;

[0055] 2) Rooting culture: cut out a single test-tube plantlet that is higher than 2.5 cm and has 2 to 3 compound leaves, and insert it into the rooting medium for 30 days; wherein:

[0056] The formula of the rooting medium is: improved MS+IBA 0.2 mg / L+IAA 2.0 mg / L+ diethyl hexanoate 0.02 mg / L+ sucrose 15 g / L+ agar 5 g / L, pH 6.0.

[0057] The culture conditions in the rooting medium are as follows: the temperature is 25° C., the light cycle is 10 h / d, and the light intensity is 3000 Lx.

[0058] After calculation, the rooting rate is 78%.

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Abstract

The invention discloses a rooting culture medium for cyclocarya paliurus (batal.) iljinskaja test-tube plantlets and a tissue culture rapid propagation method for cyclocarya paliurus (batal.) iljinskaja. According to a formula, the rooting culture medium is prepared from modified MS, 0.2-1.5 mg / L of IBA, 0-2.0 mg / L of IAA, 0.02-0.1 mg / L of diethyl aminoethyl hexanoate, 15-20 g / L of saccharose and 5-8 g / L of agar, and pH is 5.5-6.0. According to a formula, the modified MS is prepared from 700 mg / L of potassium nitrate, 600 mg / L of ammonium nitrate, 185 mg / L of magnesium sulfate heptahydrate, 60 mg / L of monopotassium phosphate and 165 mg / L of calcium chloride dehydrate. An organic compound is prepared from 100 mg / L of inositol, 1 mg / L of nicotinic acid, 1 mg / L of pyridoxine hydrochloride, 0.2 mg / L of thiamine hydrochloride and 2 mg / L of glycine. Components and concentration of microelements are the same as those of the MS culture medium. The culture medium is used for carrying out rooting culture on the cyclocarya paliurus (batal.) iljinskaja test-tube plantlets, and the rooting rate is equal to or larger than 72%.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a rooting medium for test-tube seedlings of Cyclocarya paliurus and a method for tissue culture and rapid propagation of Cyclocarya paliurus. Background technique [0002] Cyclocarya paliurus (Batal.) Iljinskaja is a plant of the genus Cyclocarya paliurus (Batal.) Iljinskaja in the Juglandaceae family. Known as the "giant panda of plants" and "the third tree in the medical field", Cyclocarya paliurus is a rare tree species that survived the Quaternary Ice Age. It only exists in China and is an endangered plant under national key protection. Cyclocarya paliurus is mainly distributed in Jiangxi, Zhejiang, Jiangsu, Anhui, Fujian, Taiwan, Hubei, Sichuan, Guizhou, Yunnan and other places, mostly scattered in deep mountains and old forests and some nature reserves. [0003] Cyclocarya paliurus has unique health care and medicinal value. Folks have long used the green papaya leaves t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 莫海萍苏玉卿李伯林刘云
Owner GUILIN BOLIN BIOLOGICAL TECH CO LTD
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