Preparation method of plant placenta extracts
A technology of plant placenta and lotus placenta, which is applied in skin care preparations, pharmaceutical formulas, cosmetic preparations, etc., to achieve effective transdermal absorption, small molecular weight, and increased antioxidant and stability effects
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Embodiment 1
[0018] Embodiment 1: the preparation method of this plant placenta comprises the following steps:
[0019] (1) Remove impurities from the fresh water lily placenta, clean it, drain the water and beat for 3 minutes at a speed of 18,000r / min, then ultrasonically treat it at 200w for 12 minutes under a stirring state of a speed of 30r / min, and then beat it at a speed of 20,000r / min High-speed homogenization for 7 minutes;
[0020] (2) Centrifuge the homogenized suspension at a high speed of 10000r / min for 3min, and take the supernatant for later use;
[0021] (3) Measure the pH of the supernatant in step (2), then adjust the pH of the supernatant to 4, increase the temperature of the solution at 40°C, add activated carbon with 1% of the solution mass and stir at 50r / min for decolorization treatment, and the treatment time For 30min, then adjust the pH of the solution to the pH of the supernatant with sodium hydroxide;
[0022] (4) Select a dialysis membrane with a molecular cut...
Embodiment 2
[0026] Embodiment 2: the preparation method of this plant placenta comprises the following steps:
[0027] (1) Remove impurities from the fresh water lily placenta, clean it, drain the water and beat for 2 minutes at a speed of 25000r / min, then ultrasonically treat it at 400w for 10min under a stirring state of a speed of 50r / min, and then beat it at a speed of 21000r / min Homogenize at high speed for 5 minutes;
[0028] (2) Centrifuge the homogenized suspension at a high speed of 20000r / min for 2min, and take the supernatant for later use;
[0029] (3) Measure the pH of the supernatant in step (2), then adjust the pH of the supernatant to 3, increase the temperature of the solution at 50°C, add activated carbon with 0.5% of the solution mass and stir at 30r / min for decolorization treatment, and the treatment time For 50min, then adjust the pH of the solution to the pH of the supernatant with sodium hydroxide;
[0030] (4) Select a dialysis membrane with a molecular cut-off o...
Embodiment 3
[0034] Embodiment 3: the preparation method of this plant placenta and compound solution comprises the following steps:
[0035] (1) Remove impurities from the fresh water lily placenta, clean it, drain the water and beat for 3 minutes at a speed of 30,000r / min, then ultrasonically treat it at 600w for 8 minutes under a stirring state of a speed of 70r / min, and then beat it at a speed of 22000r / min Homogenize at high speed for 5 minutes;
[0036] (2) Centrifuge the homogenized suspension at a high speed at 40000r / min for 3min, and take the supernatant for later use;
[0037] (3) Measure the pH of the supernatant in step (2), then adjust the pH of the supernatant to 5, increase the temperature of the solution at 45°C, add activated carbon with 0.8% of the solution mass and stir at 80r / min for decolorization treatment, and the treatment time For 30min, then adjust the pH of the solution to the pH of the supernatant with sodium hydroxide;
[0038] (4) Select a dialysis membrane...
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