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Preparation method of animal testicle extract

A technology of extract and testis, which is applied in the field of preparation of animal testis extract, can solve the problems of low extraction efficiency and product activity, unrefined extraction products, loss of active ingredients, etc., achieve less impurities, easy recovery, and increase active ingredients The effect of content

Active Publication Date: 2016-11-16
HANGZHOU HUADI GRP CO LTD
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The currently disclosed testis extraction techniques are mostly realized by water extraction or enzyme-assisted extraction methods, and the extracted products are not refined
Moreover, because testosterone is insoluble in water, the existing extraction method has lost more than 90% of testosterone, resulting in a great loss of active ingredients, and the extraction efficiency and product activity are relatively low

Method used

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  • Preparation method of animal testicle extract
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preparation example Construction

[0068] The invention discloses a preparation method of an animal testis extract, and those skilled in the art can refer to the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0069] Specific detection methods for each indicator:

[0070] A. Polypeptide content: the peptide content was detected by Folin phenol assay.

[0071] The reagents used and the specific ope...

Embodiment 1

[0085] Embodiment 1 Comparison of separation effect of different ligand agarose gel adsorption chromatography

[0086] 1. Preparation of sheep testis enzymolysis extraction refined solution

[0087] (1) Take 5 kg of freshly frozen rabbit testicles, remove fat and rinse to obtain a net weight of 3.86 Kg. Add an equal weight of purified water, and homogenize twice at room temperature.

[0088] (2) Adjust the pH of the homogenate to 9.0, add 3.8 g (0.1%) of alkaline protease, and carry out enzymatic hydrolysis at 50° C. for 3 hours. After the reaction, denature at 95°C for 5 minutes to terminate the enzymatic hydrolysis reaction, and cool to room temperature.

[0089] (3) Adjust the pH of the enzymolysis solution to 7.5, add 7.6g (0.2%) trypsin, and after 2 hours of enzymolysis at 37°C, raise the temperature to 85°C, keep it for 15 minutes, filter it through a 300-mesh non-woven fabric, and obtain the crude extract of enzymolysis Liquid and filter residue 1.6kg.

[0090] (4) ...

Embodiment 2

[0107] Example 2 Suckling pig testis enzymatic extraction

[0108] 1. Preparation of the refined solution of sucking pig testis by enzymatic hydrolysis

[0109] (1) Take 4.2 kg of freshly frozen suckling pig testicles, remove the fat and rinse to obtain a net weight of 3.1 kg, add an equal weight of distilled water, and homogenize twice at room temperature.

[0110] (2) After adjusting the pH of the homogenate to 7.5, add 9g (0.3%) trypsin, and after enzymatic hydrolysis at 37°C for 3 hours, heat up to 90°C, keep warm for 10min to denature the enzyme, cool to room temperature, and filter with 300-mesh non-woven fabric , to obtain suckling pig testis enzymolysis crude extract and filter residue 1.3kg.

[0111] (3) The above enzymolysis crude extract was clarified by ultrafiltration with a 10KD ultrafiltration membrane to obtain 4.83 liters of a light yellow clear and transparent solution with a polypeptide content of 36.35 mg / mL.

[0112] (4) The above-mentioned ultrafiltrate...

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Abstract

The invention relates to the technical field of polypeptide extraction, in particular to a preparation method of an animal testicle extract. The preparation method of the animal testicle extract comprises the following steps: homogenizing an animal testicle, and conducting enzymatic hydrolysis and extraction, so that an enzymatically hydrolyzed crude extracting solution and filter residue are obtained; conducting ultra-filtration on the enzymatically hydrolyzed crude extracting solution, so that an ultrafiltrate is obtained, and removing high-molecular impurities of the ultrafiltrate by virtue of agarose gel hydrogen bond adsorption chromatography, so that an enzymatically hydrolyzed and extracted refined solution is obtained; extracting and concentrating the filter residues by virtue of ethanol, so that a concentrated alcohol extracting solution is obtained; and mixing the enzymatically hydrolyzed and extracted refined solution and the concentrated alcohol extracting solution, and conducting freeze-drying, so that the animal testicle extract is obtained. According to the testicle extraction preparation process disclosed by the invention, by virtue of a method combining tool enzyme extraction, ultra-filtration and agarose gel hydrogen bond adsorption chromatography separation and purification, small-molecular polypeptide active substances are enriched, and testosterone, which is insoluble in water, is recovered through alcohol extraction and concentration of the filter residues, so that the loss of active ingredients is reduced. The method is simple and convenient and is safe and efficient, and a finished product (the animal testicle extract) is good in bio-activity.

Description

technical field [0001] The invention relates to the technical field of polypeptide extraction, in particular to a preparation method of animal testis extract. Background technique [0002] With the acceleration of the pace of modern life, social competition is becoming increasingly fierce, interpersonal relationships are complicated and tense, and the sub-health state caused by people's psychological imbalance is becoming more and more serious. The sub-health state refers to the state of the body with no clinical symptoms or mild symptoms, but with potential pathological information. This is a type of inferior health state, bordering between health and disease. The World Health Organization calls the state of the body without organic disease, but with some functional changes, "the third state", which is a sub-health state. The sub-health state is manifested as symptoms of decreased vitality, decreased function and adaptive capacity for a certain period of time. People in a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A23J1/02A23J3/04A61K31/568A61K35/48
Inventor 周天琼高浚铭周正兵张治国何滨霞
Owner HANGZHOU HUADI GRP CO LTD
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