Method for preparing Fuzhuan tea polysaccharide with intestinal tract benefiting function

A technology of Fu brick tea and tea polysaccharide is applied in the field of preparing Fu brick tea tea polysaccharide with intestinal probiotic function, and achieves the effect of good decolorization effect and significant intestinal probiotic function.

Inactive Publication Date: 2016-12-07
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports on polysaccharides with intestinal beneficial activities extracted from Fuzhuan tea.

Method used

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  • Method for preparing Fuzhuan tea polysaccharide with intestinal tract benefiting function
  • Method for preparing Fuzhuan tea polysaccharide with intestinal tract benefiting function
  • Method for preparing Fuzhuan tea polysaccharide with intestinal tract benefiting function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) After pulverizing the Fuzhuan tea through a 60-mesh sieve, remove the pigment and polyphenols with 80% alcohol several times to remove the pigment in the Fuzhuan tea and then dry it;

[0019] (2) Extract three times in water at 70°C with a solid-liquid ratio of 10mL / g, centrifuge and combine the extracts, concentrate, add three times the volume of absolute ethanol for alcohol precipitation, alcohol precipitation at 4°C overnight, filter, and collect the precipitate ;

[0020] (3) dissolving the precipitate in step (2) in deionized water, using the Sevage method to remove the protein in the sample, and then freeze-drying to obtain the crude product of Fuzhuan tea polysaccharide;

[0021] (4) The crude polysaccharide in step (3) was dissolved in deionized water and centrifuged, then loaded on a DEAE Sepharose FastFlow column for decolorization, eluted with 1M NaCl at a flow rate of 2mL / min, and detected by the phenol-sulfuric acid method The polysaccharide content in...

Embodiment 2

[0024] (1) After pulverizing the Fuzhuan tea through a 60-mesh sieve, remove the pigment and polyphenols with 80% alcohol several times to remove the pigment in the Fuzhuan tea and then dry it;

[0025] (2) Extract three times in water at 90°C with a solid-liquid ratio of 20mL / g, centrifuge and combine the extracts, concentrate, add three times the volume of absolute ethanol for alcohol precipitation, alcohol precipitation at 4°C overnight, filter, and collect the precipitate ;

[0026] (3) dissolving the precipitate in step (2) in deionized water, using the Sevage method to remove the protein in the sample, and then freeze-drying to obtain the crude product of Fuzhuan tea polysaccharide;

[0027] (4) The crude polysaccharide in step (3) was dissolved in deionized water and centrifuged, then loaded on a DEAE Sepharose FastFlow column for decolorization, eluted with 1M NaCl at a flow rate of 2mL / min, and detected by the phenol-sulfuric acid method The polysaccharide content in...

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Abstract

The invention relates to a method for preparing Fuzhuan tea polysaccharide with an intestinal tract benefiting function. The Fuzhuan tea polysaccharide is obtained through decoloration, drying, smashing, hot water extraction, ethanol precipitation, redissolving, DEAE Sepharose Fast Flow resin decoloration, dialysis, concentration and freeze drying of 80% ethyl alcohol. In-vitro anaerobic fermentation experiments prove that the Fuzhuan tea polysaccharide has the intestinal tract benefiting function. The method has the advantages that utilization rate is high, decoloration effect is good, the process is mild and does not damage the activity of tea polysaccharide, and a new approach is provided for developing and utilizing Fuzhuan tea polysaccharide.

Description

technical field [0001] The invention relates to the technical field of extracting biologically active polysaccharides, in particular to a method for preparing Fuzhuan tea polysaccharides with intestinal beneficial function. Background technique [0002] Fuzhuan tea is one of the six types of basic tea in my country, and it is a kind of pressed black tea. The unique traditional tea in China has a long history. Due to the unique "blooming" process of Fuzhuan brick tea through steam, machine pressure, and temperature and humidity regulation in the drying room, it promotes microbial metabolism and other functions, causing deep benign physiological and biochemical reactions in the tea, forming a luxuriant golden flower inside the Fuzhuan brick tea, with a fungal fragrance. Rich and special quality, it belongs to post-fermentation tea, and its production area is vast. Fuzhuan brick tea is mainly sold to minority-inhabited areas in my country's Tibet, Inner Mongolia, Xinjiang, Sic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L33/125
CPCC08B37/0003A23V2002/00A23V2200/3202A23V2250/214A23V2250/51A23V2300/14
Inventor 曾晓雄陈贵杰叶红
Owner NANJING AGRICULTURAL UNIVERSITY
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