Method for preparing recombinant bacillus subtilis plasmin and enteric capsule thereof

Abstract

The invention discloses a method for preparing recombinant bacillus subtilis plasmin and an enteric capsule thereof. The method comprises the following steps: 1) recombining fermentation of bacillus subtilis plasmin by using bacillus subtilis plasmin recombinant yeast, and establishing a separation purification method; 2) purifying recombined yeast fermentation broth by using hydrophobic chromatography packing so as to obtain a purified liquid; 3) performing desalination chromatography treatment on the purified liquid so as to obtain purified recombinant bacillus subtilis plasmin; 4) scanning a freezing drying protection agent, and establishing a vacuum freezing drying process; 5) filling recombinant bacillus subtilis plasmin freeze-dried powder obtained after vacuum freeze-drying on the recombinant bacillus subtilis plasmin purified liquid with the protection agent into a capsules, thereby obtaining the enteric capsules. By adopting the method, the bacillus subtilis plasmin freeze-dried powder which is highly purified can be obtained, and the purity of the powder can be 95% or higher. The processes for separating and purifying recombinant plasmin and preparing the enteric capsules are simple in procedure and easy in industrial amplification, and have great market development prospects and commercial values.

Description

technical field [0001] The invention relates to a preparation method of bacillus subtilis fibrinolytic enzyme and capsule thereof, in particular to a preparation method of recombinant bacillus subtilis fibrinolytic enzyme and enteric-coated capsule thereof, belonging to the technical field of biopharmaceuticals. Background technique [0002] Cardiovascular and cerebrovascular diseases have become the number one killer of human health. The number of deaths due to cardiovascular and cerebrovascular diseases in my country is as high as 7 million every year, and the total number of patients has exceeded 300 million. In my country alone, the market related to cardiovascular and cerebrovascular diseases has exceeded 1 trillion yuan. The main cause behind cardiovascular and cerebrovascular diseases is the formation of thrombus. [0003] Currently, there are three main methods for the treatment of thromboembolic diseases: surgery, conservative therapy, and thrombolytic therapy. Th...

AI Technical Summary

Problems solved by technology

Since the discovery of Bacillus subtilis plasmin in the 1980s, it has been relying on traditional soybean solid fermentation, which is difficult to achieve a large amount of separation and purification of Bacillus subtilis plasmin

Due to the extremely low solid fermentation content and the extremely complex fermentation products, the purification cost is bound to be extremely high

Therefore, Bacillus subtilis fibrinolytic enzyme has been used in the development of health food field, and has not exerted its own important medicinal value

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