46results about How to "Easy to scale up for industrialization" patented technology

The present invention relates to a method for producing recombinant human serum albumin-interferon alpha 2b of secretory expression in Pichia pastoris system, particularly to efficient expression of recombinant human serum albumin-interferon alpha 2b in Pichia pastoris and expression product purification. In the present invention, Pichia pastoris engineering bacteria containing recombinant human serum albumin-interferon alpha 2b are fermented in high density, fermented liquid is subsequently centrifuged by a high-speed centrifugal machine, and fermented supernatant liquid is collected and purified by cation exchange chromatography, hydrophobic layer chromatography and molecular sieve chromatography in a combined mode. Recombinant human serum albumin-interferon alpha 2b fusion protein produced meets the regulations of Chinese Pharmacopoeia. The present invention has the advantages of high fermentation expression rate, reasonable purification technical combination, simplified operation procedure, high yield and low production cost. The present invention meets industrial requirements.

The invention discloses a method for renaturing and purifying novel thrombopoietin mimetic peptide, the method comprises the following steps: performing a high density fermentation to escherichia coli engineering bacteria containing thrombopoietin mimetic peptide, carrying out an ultrasonication to thalline, urea cracking, renaturing, regulating the pH value, combining and purifying through Protein-AMabselectSuRe chromatography, SPSepharoseH.P chromatography and Sephacry1S-200 molecular sieve chromatography, and preparing thrombopoietin mimetic peptide protein which conforms to the Chinese pharmacopoeia specification. The beneficial effects of the present invention have high renaturation rate, reasonable purifying process combination, simplified operation program, high yield and low production cost, and the method provides the possibility for the large scale production of the thrombopoietin mimetic peptide.

The invention provides a molten bath and a method for recycling thermosetting epoxy resin or a composite material by using the same. The molten bath comprises at least one alkali metal hydroxide selected from lithium hydroxide, sodium hydroxide and potassium hydroxide and at least one additive, wherein the weight percentage ratio of the alkali metal hydroxide to the additive is (80-99.9): (0.1-20). The invention also provides a method for recycling the epoxy resin or the composite material by using the molten bath. By adopting the method, the thermosetting epoxy resin and the composite material thereof can be effectively decomposed under normal pressure, separation of the epoxy resin from an inorganic material is realized, the problems of low treatment efficiency and poor economic efficiency existing in the recycling process of old and useless epoxy resin and a composite material thereof are solved, and the decomposition rate of the epoxy resin or the epoxy resin in the composite material thereof is up to 90-100 percent. In the invention, industrialization is easy to amplify in a technical process, and a resource technology for effectively recycling epoxy resin or the composite material thereof is provided.

The invention relates to the technical field of biological medicines, and particularly relates to a pyridine sulfonamide phosphate compound as well as a preparation method and application thereof. Thestructure of the pyridine sulfonamide phosphate compound is shown as a formula I shown in the specification. The pyridine sulfonamide phosphate compound has the beneficial effects that the pyridine sulfonamide phosphate compound has the characteristics of high solubility, high stability, convenience in preparation of preparations and the like, is easy to industrially amplify and is used for medical application.

The invention discloses a method of extracting and purifying bergenin from cissus pteroclada hayata. The method comprises the steps that the cissus pteroclada hayata is smashed and screened, ethanol solution is added according to the mass-to-volume ratio of 1 to 8-20 for reflux extraction, solid-liquid separation is conducted to obtain filtrate, the filtrate is concentrated, macroporous resin is added into the concentrated solution for static adsorption, the macroporous resin subjected to adsorption is placed on a pillar, impurities are removed and elution is conducted with distilled water and ethanol solution in sequence, the eluant is collected and concentrated to obtain crude bergenin, and the crude bergenin is recrystallized with ethanol to obtain pure bergenin. According to the method, sources of raw materials are wide, price is low, production cost is low, production conditions are moderate, and pollution to the environment is light.

The invention discloses an expanded bed chromatographic separation device for a biochemical separation technology, belonging to the biochemical field. A liquid distributor consisting of a feeding pipe, a radial spraying hole and a radial guiding disk is arranged under an expanded bed porous screen / sieve plate, and the bottom end of the expanded bed is provided with a material discharging hole. Through changing a circulation area of fluid flowing along a radial direction of the guiding disk, the liquid distribution pressure change caused by variable mass flowing and a runner changing in a fan-shaped geometrical shape is overcome / offset, the problem of uniform distribution of liquid supply pressure of feed liquid in the radius direction under low bed pressure drop in an industrial amplification production process of the expanded bed is solved, the fluid passing the bed is ensured to be in a flat pushing flow state, the use effect and the use efficiency of the expanded bed are greatly improved, and the adsorption capacity and the desorption resolution ratio of the expanded bed are improved. The invention ensures that the expanded bed technology can be applied to purification and separation of all protide substances produced by microorganisms and animal cells and solid particles or materials of sediments are directly used in the chromatographic separation.

The invention discloses an extraction method of acridine coumarin alkali F. The extraction method comprises the following steps: (1) taking coarse root powder of grapefruit hybrid oranges and 85-95% of ethanol solution to carry out continuous counter-current ultrasonic extraction so as to obtain an extracting solution; (2) concentrating the extracting solution, and then carrying out alumina column chromatography on the extracting solution; (3) carrying out preparative liquid chromatographic separation on the obtained object; and (4) carrying out chloroform-methanol recrystallization on the obtained product so as to obtain a high-purity acridine coumarin alkali F crystal product. The method disclosed by the invention is simple and high in yield, can obtain high-purity products, and has high economic and academic values.

The invention provides a molten bath and a method for recycling thermosetting epoxy resin or a composite material by using the same. The molten bath comprises at least one alkali metal hydroxide selected from lithium hydroxide, sodium hydroxide and potassium hydroxide and at least one additive, wherein the weight percentage ratio of the alkali metal hydroxide to the additive is (80-99.9): (0.1-20). The invention also provides a method for recycling the epoxy resin or the composite material by using the molten bath. By adopting the method, the thermosetting epoxy resin and the composite material thereof can be effectively decomposed under normal pressure, separation of the epoxy resin from an inorganic material is realized, the problems of low treatment efficiency and poor economic efficiency existing in the recycling process of old and useless epoxy resin and a composite material thereof are solved, and the decomposition rate of the epoxy resin or the epoxy resin in the composite material thereof is up to 90-100 percent. In the invention, industrialization is easy to amplify in a technical process, and a resource technology for effectively recycling epoxy resin or the composite material thereof is provided.

The invention belongs to the technical field of a preparation method for high-purity aromatic hydrocarbon pitch used for a high-end carbon material, and specifically relates to a preparation method for low-ash, low-sulfur and high-purity aromatic hydrocarbon pitch used for preparation of mesophase pitch. The preparation method comprises the following steps: dissolving aromatic hydrocarbon pitch into toluene, then adding a boron trifluoride-diethyl ether complex, carrying out stirring for layering at a normal temperature, separating out a high-density boron trifluoride-aromatic hydrocarbon pitch complex obtained at a bottom part, then adding the toluene and pyridine, further carrying out stirring for layering, removing a majority of the boron trifluoride, adding ammonia water and low-molecular alcohol or a surfactant, carrying out extraction for a plurality of times, and carrying out distillation so as to obtain the high-purity aromatic hydrocarbon pitch. The preparation method provided by invention is performed at a normal temperature, has low energy consumption and good purification and separation effects, is simple in equipment and low in investment and cost, and avoids the disadvantages of a conventional filtration and hydrogenation process.

The invention discloses a method for preparing a high-purity agaricus blazei murrill polysaccharide and provides a method capable of obtaining high content of the agaricus blazei murrill polysaccharide and suitable for large-scale production. The method comprises the following steps of: adding cellulase and pectinase to an agaricus blazei murrill powder; after adding water to the agaricus blazei murrill powder and uniformly stirring the water and the agaricus blazei murrill powder, regulating the pH value of a mixture to 6.0+ / -0.1; performing enzymolysis on the mixture for 40 minutes; carrying out microwave extraction on the mixture; freezing and drying agaricus blazei murrill water extract so as to obtain a concentrated product; carrying out decolorizing and primary impurity removal treatment on an agaricus blazei murrill polysaccharide extract solution by using a macroporous adsorbing resin; and then carrying out deproteinization treatment on the agaricus blazei murrill polysaccharide extract solution by using an ion exchange resin; further purifying the agaricus blazei murrill polysaccharide after deproteinization by using an ultrafiltration technology, so as to eliminate micromolecular water soluble substances; pre-freezing an agaricus blazei murrill polysaccharide ultrafiltrate to -60 DEG C; maintaining the agaricus blazei murrill polysaccharide ultrafiltrate at -60 DEG C for at least 4 hours; and drying the agaricus blazei murrill polysaccharide ultrafiltrate at -45 DEG C in a vacuum state so as to obtain the agaricus blazei murrill polysaccharide. Through the adoption of a microwave synergistic complex enzyme method, a resin deproteinization technology, an ultrafiltration process purification and freeze-drying technology, which are combined with each other, the purity of the agaricus blazei murrill polysaccharide reaches 90%.

The invention discloses a method for preparing a graphene carbon nanotube composite conductive framework for a lithium-sulfur battery. The method comprises the following steps: S1, preparing mixed slurry from graphene oxide and carbon nanotue powder; S2, adding a surfactant, mixing, and dispersing so as to obtain a graphene oxide carbon nanotube dispersant; S3, adding an adhesive and a curing agent, mixing, and dispersing so as to obtain a precursor solution; S4, evaporating the solution of the precursor solution, and performing curing treatment so as to obtain graphene oxide carbon nanotube composite precursor powder; and S5, performing high-temperature carbonization reduction, thereby obtaining a graphene oxide carbon nanotube three-dimensional composite conductive framework. The methodis simple and efficient in process step, low in cost and easy in industrial application, the microstructure carbon nanotubes of the prepared graphene oxide carbon nanotube composite powder are dispersed on the surface of graphene, a single-layer dispersion structure of the graphene is maintained, a good synergetic effect between the graphene and the carbon nanotubes is achieved, and the frameworkis an efficient three-dimensional conductive framework.

The invention relates to an expanded bed chromatographic separation column used for a biochemical separation process and a process flow, belonging to the field of biochemistry. In the invention, a liquid pressure equalizing distributor with self-cleaning function is arranged below a porous blocking sieve plate at the bottom of an expanded bed, and an upper nozzle assembly with back-flushing cleaning function is arranged at the top of the expanded bed so that the expanded bed chromatographic separation column is suitable for treating a raw material liquid containing solid particle impurities, meanwhile, the raw material sample liquid entering the expanded bed can be better distributed uniformly, therefore, the problem of uniform distribution of feed liquid pressure of lower-bed pressure drop feed liquid along a radius direction in the industrial amplification production process is solved, fluid passing through a bed layer of the expanded bed is better ensured to be in a plug flow state, the separation theoretical plate number and the chromatographic separation efficiency of the expanded bed can be greatly enhanced, the using effect and the using efficiency of the expanded bed are improved and the adsorption capacity and the desorption resolution of the expanded bed are enhanced.

The invention discloses a preparation method of acronycine. The preparation method comprises the following steps of (1) weighting coarse powder of acronychia pedunculata roots, and carrying out continuous countercurrent ultrasonic extraction by using 70-95% ethanol solution to obtain an extracting solution; (2) concentrating the extracting solution, and carrying out aluminum oxide column chromatography; (3) carrying out preparative liquid chromatographic separation; (4) carrying out chloroform and acetone recrystallization to obtain a high-purity acronycine crystal product. The method disclosed by the invention is simple, high in acronycine yield and capable of obtaining high-purity products; in addition, the acronycine has relatively high economic and academic values.

The present invention provides a process and system for producing natural gas from coal-fired self-heating catalytic gasification. Coal, catalyst and gasification agent are supplied to a gasification device, wherein the gasification agent is distributed and supplied from different positions of the gasification device to the gasification device. gasification unit, and oxygen is introduced into the gasification unit. According to the process for producing natural gas from coal-fired self-heating coal catalytic gasification disclosed in the present invention, the coal-fired self-heating method can provide the required heat for the catalytic gasification reaction, no additional heating equipment is required, the process is simple, and the technical economy is good , the single furnace realizes heat coupling, and the system efficiency is high; in addition, by providing gasification agent dispersedly to the gasification device, while providing the heat required for the reaction, it can control the oxygen distribution in the whole bed and adjust the oxygen distribution in the distribution plate and the bed. The ratio of steam and oxygen is used to control the oxygen concentration within a certain range, which can avoid local high temperature areas in the bed, thereby effectively avoiding slagging.

The invention discloses a water-soluble β-glucan, its preparation method and its application in the preparation of immune-enhancing and anti-tumor medicines and health care products. The present invention first prepares alkaline urea aqueous solution and freezes it, then adds insoluble β-glucan, freezes after stirring and dissolving, then heats to an appropriate temperature, adds hydrogen peroxide aqueous solution dropwise under stirring, adjusts pH to neutrality, and performs dialysis. The dialyzed internal fluid is concentrated under reduced pressure and freeze-dried to obtain water-soluble β-glucan. The method solves the technical problem that the insoluble β-glucan is difficult to degrade through the homogeneous degradation in the alkaline urea aqueous solution. The water-soluble β-glucan obtained by using the method can significantly enhance the immune activity of macrophage RAW 264.7 and effectively antagonize tumor cells. The β-glucan prepared by the present invention has the advantages of easy-to-obtain raw materials, simple preparation process, strong biological activity, and easy industrial production, and has the prospect of being developed as an immune-enhancing and anti-tumor drug.

The present invention relates to a method for producing recombinant human serum albumin-interferon alpha 2b of secretory expression in Pichia pastoris system, particularly to efficient expression of recombinant human serum albumin-interferon alpha 2b in Pichia pastoris and expression product purification. In the present invention, Pichia pastoris engineering bacteria containing recombinant human serum albumin-interferon alpha 2b are fermented in high density, fermented liquid is subsequently centrifuged by a high-speed centrifugal machine, and fermented supernatant liquid is collected and purified by cation exchange chromatography, hydrophobic layer chromatography and molecular sieve chromatography in a combined mode. Recombinant human serum albumin-interferon alpha 2b fusion protein produced meets the regulations of Chinese Pharmacopoeia. The present invention has the advantages of high fermentation expression rate, reasonable purification technical combination, simplified operation procedure, high yield and low production cost. The present invention meets industrial requirements.