47results about How to "Easy to scale up for industrialization" patented technology

The invention relates to a jet pipe, a fluidized bed reactor with the jet pipe and a coal catalyzing and gasifying method. A plurality of jet pipes which are in a bed-layer dispersing manner are adopted for feeding oxygen; the jet pipes are in a sleeve type structure; a central pipe is supplied with a mixture gas of oxygen and over-hot steam; the over-hot steam is supplied into annular spaces; the ratio of the oxygen to the over-hot steam is regulated to control the oxygen concentration to be within a certain range; the oxygen and a carbon material are mainly subjected to combustion strong exothermic reaction at a jet flow area; the released heat is used for providing heat for the gasification reaction in the reactor; the over-hot steam supplied into the annular spaces can intensity the internal circulation of carbon material granules, is subjected to heat exchange with a high-temperature gas at a central pipe area, and at the same time provide heat for the gasification heat absorption reaction in the annular spaces, so that partial high-temperature areas are prevented from happening through rapid heat exchange and gasification heat absorption reaction, and the happening rate that the jet pipe area has slag build-up is reduced.

The present invention relates to a method for producing recombinant human serum albumin-interferon alpha 2b of secretory expression in Pichia pastoris system, particularly to efficient expression of recombinant human serum albumin-interferon alpha 2b in Pichia pastoris and expression product purification. In the present invention, Pichia pastoris engineering bacteria containing recombinant human serum albumin-interferon alpha 2b are fermented in high density, fermented liquid is subsequently centrifuged by a high-speed centrifugal machine, and fermented supernatant liquid is collected and purified by cation exchange chromatography, hydrophobic layer chromatography and molecular sieve chromatography in a combined mode. Recombinant human serum albumin-interferon alpha 2b fusion protein produced meets the regulations of Chinese Pharmacopoeia. The present invention has the advantages of high fermentation expression rate, reasonable purification technical combination, simplified operation procedure, high yield and low production cost. The present invention meets industrial requirements.

The invention relates to a malachite green molecularly imprinted solid phase extraction filler and a preparation method thereof. The preparation method comprises the following steps of: fully mixing a hydrophobic alkene monomer, malachite green and an oil-soluble initiator to form an oil-phase fluid which is uniformly distributed; and smashing and dispersing the oil-phase fluid into oil drops of uniform sizes with a jet suspension polymerization method, forming a uniform O / W suspension in a water phase, polymerizing at a constant temperature to obtain malachite green polymer compound microspheres, and removing malachite green template molecules to obtain the solid phase extraction filler. The solid phase extraction filler prepared with the method has uniform particle diameter, stable chemical property and high selectivity and high yield when used for detecting the residues of malachite green in foods.

The invention discloses a method for renaturing and purifying novel thrombopoietin mimetic peptide, the method comprises the following steps: performing a high density fermentation to escherichia coli engineering bacteria containing thrombopoietin mimetic peptide, carrying out an ultrasonication to thalline, urea cracking, renaturing, regulating the pH value, combining and purifying through Protein-AMabselectSuRe chromatography, SPSepharoseH.P chromatography and Sephacry1S-200 molecular sieve chromatography, and preparing thrombopoietin mimetic peptide protein which conforms to the Chinese pharmacopoeia specification. The beneficial effects of the present invention have high renaturation rate, reasonable purifying process combination, simplified operation program, high yield and low production cost, and the method provides the possibility for the large scale production of the thrombopoietin mimetic peptide.

The invention provides a molten bath and a method for recycling thermosetting epoxy resin or a composite material by using the same. The molten bath comprises at least one alkali metal hydroxide selected from lithium hydroxide, sodium hydroxide and potassium hydroxide and at least one additive, wherein the weight percentage ratio of the alkali metal hydroxide to the additive is (80-99.9): (0.1-20). The invention also provides a method for recycling the epoxy resin or the composite material by using the molten bath. By adopting the method, the thermosetting epoxy resin and the composite material thereof can be effectively decomposed under normal pressure, separation of the epoxy resin from an inorganic material is realized, the problems of low treatment efficiency and poor economic efficiency existing in the recycling process of old and useless epoxy resin and a composite material thereof are solved, and the decomposition rate of the epoxy resin or the epoxy resin in the composite material thereof is up to 90-100 percent. In the invention, industrialization is easy to amplify in a technical process, and a resource technology for effectively recycling epoxy resin or the composite material thereof is provided.

The invention relates to the technical field of biological medicines, and particularly relates to a pyridine sulfonamide phosphate compound as well as a preparation method and application thereof. Thestructure of the pyridine sulfonamide phosphate compound is shown as a formula I shown in the specification. The pyridine sulfonamide phosphate compound has the beneficial effects that the pyridine sulfonamide phosphate compound has the characteristics of high solubility, high stability, convenience in preparation of preparations and the like, is easy to industrially amplify and is used for medical application.

The invention provides a technology for preparing natural gas through self-heating catalytic gasification of fire coal, and a system thereof. Coal, a catalyst and a gasifying agent are supplied to a gasifying apparatus, wherein the gasifying agent is supplied to the gasifying apparatus in a dispersion mode from different positions of the gasifying apparatus, and oxygen is introduced into the gasifying apparatus. The technology for preparing the natural gas through self-heating catalytic gasification of fire coal, which allows heat required by a catalytic gasifying reaction to be provided in a fire coal self-heating mode, has the characteristics of no installation of a heating device, simplicity, good economy, and realization of single-furnace heat coupling, and the system has a high efficiency; and additionally, the gasifying agent is supplied to the gasifying apparatus in a dispersion mode, so heat required by the reaction is supplied, the oxygen concentration can be controlled within the specific limits through controlling the full-bed oxygen distribution and adjusting the ratio of steam to oxygen in a distribution plate and a bed in order to avoid a local high-temperature region appearing in the bed, thereby slag bonding can be effectively avoided.

The invention discloses an extraction separation method of polydatin. According to the method, polygonum cuspidatum leaves are adopted as the raw materials, technology procedures including alcohol extraction, degreasing in diethyl ether, water solution for filtering, resin column chromatography isolation and the like are adopted, and polydatin the content of which reaches 70% or above is prepared;the separation effect is remarkable, and a product the content of which reaches 98% or above can be obtained after simple recrystallization. The process of the method is simple, the recovery rate ishigh, the purity is high, and the method is suitable for industrial production.

The invention discloses a method for extracting papain by using a double aqueous phase system, belongs to the technical field of bioseparation, and particularly relates to an ionic liquid assisted polyethylene glycol / sodium dihydrogen phosphate system. The method comprises the following specific steps: taking imidazolium ionic liquid as an auxiliary material, and adding the imidazolium ionic liquid into a polyethylene glycol 400 / sodium dihydrogen phosphate double aqueous phase system prepared according to a certain mass fraction; then adding a papain solution with a certain concentration; after preparation is finished, putting the prepared solution on a vortex oscillator, oscillating for 5 minutes and uniformly mixing; subsequently, putting the mixed solution into a thermostat water bath with the temperature of 25 DEG C and allowing standing still for 24 hours; after the phase splitting operation is finished, respectively taking an upper phase and a lower phase, diluting the solution to a certain concentration with distilled water, and measuring the concentration of the papain in each phase. The method disclosed by the invention has the characteristics that the extraction rate of the papain by a polymer / salt double aqueous phase system can be remarkably improved; besides, the viscosity of the double aqueous phase system can be reduced; the method is low in energy consumption, simple in operation and the like.

The invention discloses water-soluble beta-glucan, a preparation method thereof and application of the water-soluble beta-glucan in preparation of immunopotentiation and anti-tumor drugs and health care products. The preparation method comprises the following steps: firstly, preparing an alkaline urea aqueous solution, carrying out cryopreservation, then adding insoluble beta-glucan, stirring to dissolve the beta-glucan, freezing, then heating to a proper temperature, dropwise adding a hydrogen peroxide aqueous solution while stirring, adjusting the pH value to be neutral, carrying out dialysis, and carrying out reduced pressure concentration and freeze drying on dialysis internal liquid, so as to obtain the water-soluble beta-glucan. According to the method, the technical problem that insoluble beta-glucan is difficult to degrade is solved through homogeneous degradation in an alkaline urea aqueous solution. The water-soluble beta-glucan obtained by using the method can significantlyenhance the immunocompetence of macrophages RAW 264.7 and effectively antagonize tumor cells. The beta-glucan prepared by the invention has the advantages of easily available raw materials, simple preparation process, strong biological activity, easiness in industrial production and the like, and has a prospect of being developed into immunopotentiation and anti-tumor drugs.

The invention discloses a method for preparing recombinant bacillus subtilis plasmin and an enteric capsule thereof. The method comprises the following steps: 1) recombining fermentation of bacillus subtilis plasmin by using bacillus subtilis plasmin recombinant yeast, and establishing a separation purification method; 2) purifying recombined yeast fermentation broth by using hydrophobic chromatography packing so as to obtain a purified liquid; 3) performing desalination chromatography treatment on the purified liquid so as to obtain purified recombinant bacillus subtilis plasmin; 4) scanning a freezing drying protection agent, and establishing a vacuum freezing drying process; 5) filling recombinant bacillus subtilis plasmin freeze-dried powder obtained after vacuum freeze-drying on the recombinant bacillus subtilis plasmin purified liquid with the protection agent into a capsules, thereby obtaining the enteric capsules. By adopting the method, the bacillus subtilis plasmin freeze-dried powder which is highly purified can be obtained, and the purity of the powder can be 95% or higher. The processes for separating and purifying recombinant plasmin and preparing the enteric capsules are simple in procedure and easy in industrial amplification, and have great market development prospects and commercial values.

The invention discloses a method of extracting and purifying bergenin from cissus pteroclada hayata. The method comprises the steps that the cissus pteroclada hayata is smashed and screened, ethanol solution is added according to the mass-to-volume ratio of 1 to 8-20 for reflux extraction, solid-liquid separation is conducted to obtain filtrate, the filtrate is concentrated, macroporous resin is added into the concentrated solution for static adsorption, the macroporous resin subjected to adsorption is placed on a pillar, impurities are removed and elution is conducted with distilled water and ethanol solution in sequence, the eluant is collected and concentrated to obtain crude bergenin, and the crude bergenin is recrystallized with ethanol to obtain pure bergenin. According to the method, sources of raw materials are wide, price is low, production cost is low, production conditions are moderate, and pollution to the environment is light.

The invention relates to the field of pharmaceutical preparations and provides a nimodipine nanocrystallization method and a dry suspension thereof. The method comprises the following steps: nano-crystallizing nimodipine by adopting a medium grinding method; preparing a nano suspension in the presence of a stabilizer and water; then adding a suspending agent and a spray drying protective agent orother auxiliary materials to prepare nano suspension spray drying powder by further spraying and drying; and then adding other auxiliary materials and uniformly mixing to obtain the nimodipine dry suspension. The dry suspension is good in redispersibility, good in stability and convenient to take; nimodipine which is redispersed has small grain size and narrow distribution, and the potential of nimodipine meets the demand. The dissolution rate of the drug is improved obviously, and the bioavailability in vivo of the drug can be improved. Meanwhile, the dry suspension formula and preparation process are simple, and the preparation is safe and effective, has relatively low cost and is easy for industrial production.

The invention relates to an artificial culture technique of a fungus and discloses a method for culturing a Cordyceps fungus. The method comprises the following steps: filling a closed or semienclosed culture chamber with a mixture of a culture medium and a Cordyceps fungus strain; placing the culture chamber under the culture condition suitable for the growth of the Cordyceps fungus; and obtaining a Cordyceps fungus product with the shape consistent with the chamber body, after the culture chamber is overgrown with the fungus, wherein, the capacity of the culture chamber is 0.3ml-10ml. The culture method of the invention is simple and easy, and the cultured cordyceps is more like wild cordyceps and has higher medicinal efficacy.

The invention discloses an orally-taken tuckahoe polysaccharide sulfate-chitosan nanoparticle and a preparation method thereof. The preparation method comprises the following steps of: slowly adding a tuckahoe polysaccharide sulfate solution into a chitosan solution with stirring and continuously stirring, wherein at the same time, a mixed solution becomes turbid and a stable nano colloid solution is obtained; and spraying and drying the nano colloid solution by adopting a spraying and drying method at the high temperature of 70-120DEG C to obtain the orally-taken tuckahoe polysaccharide sulfate-chitosan nanoparticle. The nano-particle prepared by the preparation method is high in drug-loading rate, is small in particle size and is high in stability under the acidic environments of which the pH value is smaller than 7.5 and the temperature is about 37DEG C; and the nano-particle can be affined with small intestine and penetrates through the small intestine at the duodenum part in a form of complete nanoparticle and the medicament slowly releases in the blood.

The invention provides a method for separating and purifying recombinant bacillus subtilis fibrinolytic enzyme. The method comprises the following steps: carrying out hydrophobic chromatography: filling a chromatographic column with phenyl-agarose-series hydrophobic filler, washing and balancing the chromatographic column with liquid A, detecting when an ultraviolet absorption wavelength is 280 nanometers, removing thallus of fermentation liquor of the recombinant bacillus subtilis fibrinolytic enzyme and then directly loading a sample into the hydrophobic chromatographic column, continuously adding the liquid A in a flowing way until a flow-through peak flows through completely, and replacing the liquid A with liquid B for eluting until the recombinant bacillus subtilis fibrinolytic enzyme combined with the filler is completely eluted so as to obtain the high-purity recombinant bacillus subtilis fibrinolytic enzyme. A bacillus subtilis fibrinolytic enzyme separation and purification method with a pilot scale test level is established in a fermentation culture solution on the basis of recombinant yeast fermentation of the bacillus subtilis fibrinolytic enzyme; after the method is used, a great deal of high-purity fibrinolytic enzyme can be obtained, and the purity of the obtained fibrinolytic enzyme reaches up to 95%.

The invention relates to a method for preparing warburganal and belongs to the field of preparation of traditional Chinese medicines. According to the method, warburganal is extracted from raw materials rich in warburganal. The method comprises the following steps of grinding the raw materials rich in warburganal, performing ultrasonic extraction by adding a hydrophilic organic solvent, recycling the organic solvent to obtain an extract, performing chromatographic separation by using a silica gel alumina mixed column for two times, performing gradient elution by adding the mixed solvent, recycling the solvent, standing a solution for crystallization, and performing re-crystallization by adding petroleum ether-acetone or cyclohexane-methanol to obtain the product. The method has the advantages that the yield is high, a closed system is adopted in the extracting process, the consumption of the solvent is reduced, and the production safety is improved.

The invention discloses an expanded bed chromatographic separation device for a biochemical separation technology, belonging to the biochemical field. A liquid distributor consisting of a feeding pipe, a radial spraying hole and a radial guiding disk is arranged under an expanded bed porous screen / sieve plate, and the bottom end of the expanded bed is provided with a material discharging hole. Through changing a circulation area of fluid flowing along a radial direction of the guiding disk, the liquid distribution pressure change caused by variable mass flowing and a runner changing in a fan-shaped geometrical shape is overcome / offset, the problem of uniform distribution of liquid supply pressure of feed liquid in the radius direction under low bed pressure drop in an industrial amplification production process of the expanded bed is solved, the fluid passing the bed is ensured to be in a flat pushing flow state, the use effect and the use efficiency of the expanded bed are greatly improved, and the adsorption capacity and the desorption resolution ratio of the expanded bed are improved. The invention ensures that the expanded bed technology can be applied to purification and separation of all protide substances produced by microorganisms and animal cells and solid particles or materials of sediments are directly used in the chromatographic separation.

The invention discloses an extraction method of acridine coumarin alkali F. The extraction method comprises the following steps: (1) taking coarse root powder of grapefruit hybrid oranges and 85-95% of ethanol solution to carry out continuous counter-current ultrasonic extraction so as to obtain an extracting solution; (2) concentrating the extracting solution, and then carrying out alumina column chromatography on the extracting solution; (3) carrying out preparative liquid chromatographic separation on the obtained object; and (4) carrying out chloroform-methanol recrystallization on the obtained product so as to obtain a high-purity acridine coumarin alkali F crystal product. The method disclosed by the invention is simple and high in yield, can obtain high-purity products, and has high economic and academic values.

The invention belongs to the technical field of a preparation method for high-purity aromatic hydrocarbon pitch used for a high-end carbon material, and specifically relates to a preparation method for low-ash, low-sulfur and high-purity aromatic hydrocarbon pitch used for preparation of mesophase pitch. The preparation method comprises the following steps: dissolving aromatic hydrocarbon pitch into toluene, then adding a boron trifluoride-diethyl ether complex, carrying out stirring for layering at a normal temperature, separating out a high-density boron trifluoride-aromatic hydrocarbon pitch complex obtained at a bottom part, then adding the toluene and pyridine, further carrying out stirring for layering, removing a majority of the boron trifluoride, adding ammonia water and low-molecular alcohol or a surfactant, carrying out extraction for a plurality of times, and carrying out distillation so as to obtain the high-purity aromatic hydrocarbon pitch. The preparation method provided by invention is performed at a normal temperature, has low energy consumption and good purification and separation effects, is simple in equipment and low in investment and cost, and avoids the disadvantages of a conventional filtration and hydrogenation process.

The invention discloses a method for preparing a high-purity agaricus blazei murrill polysaccharide and provides a method capable of obtaining high content of the agaricus blazei murrill polysaccharide and suitable for large-scale production. The method comprises the following steps of: adding cellulase and pectinase to an agaricus blazei murrill powder; after adding water to the agaricus blazei murrill powder and uniformly stirring the water and the agaricus blazei murrill powder, regulating the pH value of a mixture to 6.0+ / -0.1; performing enzymolysis on the mixture for 40 minutes; carrying out microwave extraction on the mixture; freezing and drying agaricus blazei murrill water extract so as to obtain a concentrated product; carrying out decolorizing and primary impurity removal treatment on an agaricus blazei murrill polysaccharide extract solution by using a macroporous adsorbing resin; and then carrying out deproteinization treatment on the agaricus blazei murrill polysaccharide extract solution by using an ion exchange resin; further purifying the agaricus blazei murrill polysaccharide after deproteinization by using an ultrafiltration technology, so as to eliminate micromolecular water soluble substances; pre-freezing an agaricus blazei murrill polysaccharide ultrafiltrate to -60 DEG C; maintaining the agaricus blazei murrill polysaccharide ultrafiltrate at -60 DEG C for at least 4 hours; and drying the agaricus blazei murrill polysaccharide ultrafiltrate at -45 DEG C in a vacuum state so as to obtain the agaricus blazei murrill polysaccharide. Through the adoption of a microwave synergistic complex enzyme method, a resin deproteinization technology, an ultrafiltration process purification and freeze-drying technology, which are combined with each other, the purity of the agaricus blazei murrill polysaccharide reaches 90%.

The invention relates to an expanded bed chromatographic separation column used for a biochemical separation process and a process flow, belonging to the field of biochemistry. In the invention, a liquid pressure equalizing distributor with self-cleaning function is arranged below a porous blocking sieve plate at the bottom of an expanded bed, and an upper nozzle assembly with back-flushing cleaning function is arranged at the top of the expanded bed so that the expanded bed chromatographic separation column is suitable for treating a raw material liquid containing solid particle impurities, meanwhile, the raw material sample liquid entering the expanded bed can be better distributed uniformly, therefore, the problem of uniform distribution of feed liquid pressure of lower-bed pressure drop feed liquid along a radius direction in the industrial amplification production process is solved, fluid passing through a bed layer of the expanded bed is better ensured to be in a plug flow state, the separation theoretical plate number and the chromatographic separation efficiency of the expanded bed can be greatly enhanced, the using effect and the using efficiency of the expanded bed are improved and the adsorption capacity and the desorption resolution of the expanded bed are enhanced.

The invention discloses a preparation method of acronycine. The preparation method comprises the following steps of (1) weighting coarse powder of acronychia pedunculata roots, and carrying out continuous countercurrent ultrasonic extraction by using 70-95% ethanol solution to obtain an extracting solution; (2) concentrating the extracting solution, and carrying out aluminum oxide column chromatography; (3) carrying out preparative liquid chromatographic separation; (4) carrying out chloroform and acetone recrystallization to obtain a high-purity acronycine crystal product. The method disclosed by the invention is simple, high in acronycine yield and capable of obtaining high-purity products; in addition, the acronycine has relatively high economic and academic values.

The invention discloses an anti-biological pollution ultrafiltration membrane based on quaternary ammonium salt composite layered double hydroxide (LDH) and a preparation method thereof. The preparation method comprises the following steps: firstly, embedding quaternary ammonium salt into interlayer gaps of LDH nanosheets, then blending the quaternary ammonium salt composite LDH nanosheets, a pore-foaming agent, polyether sulfone powder and a solvent to prepare a membrane casting solution, coating non-woven fabric with the membrane casting solution by using a scraper, and finally immersing the non-woven fabric coated with the membrane casting solution into deionized water for phase inversion, so as to obtain the membrane. The ultrafiltration membrane based on the quaternary ammonium salt composite layered double hydroxide is formed. According to the method, the quaternary ammonium salt and the LDH nanosheets are cheap and easy to obtain, based on microbial cell puncture of the LDH nanosheets and the intracellular release sterilization effect of the quaternary ammonium salt, the obtained ultrafiltration membrane has excellent antibacterial activity and biological pollution resistance, and the quaternary ammonium salt composite LDH nanosheets can also effectively enhance the hydrophilicity, water flux and organic pollution resistance of the membrane; according to the invention, the traditional phase inversion membrane preparation process is not changed, and the quaternary ammonium salt composite LDH nanosheet is simple and convenient to synthesize, cheap in material and easy to industrialize.

The invention discloses an asarone nanocrystallization method, and belongs to the technical field of pharmaceutical preparations. According to the nanocrystallization method, an anti-solvent precipitation method is adopted, asarone is firstly dissolved in an organic solvent and then mixed with an aqueous solution of a stabilizer, so that the asarone is supersaturated and separated out to form a stable nanoscale colloidal dispersion. The solubility and dissolution rate of asarone are improved through a nanocrystallization technology, and the problems that an asarone oral preparation is difficult to dissolve out and low in bioavailability are solved.

The present invention relates to a method for producing recombinant human serum albumin-interferon alpha 2b of secretory expression in Pichia pastoris system, particularly to efficient expression of recombinant human serum albumin-interferon alpha 2b in Pichia pastoris and expression product purification. In the present invention, Pichia pastoris engineering bacteria containing recombinant human serum albumin-interferon alpha 2b are fermented in high density, fermented liquid is subsequently centrifuged by a high-speed centrifugal machine, and fermented supernatant liquid is collected and purified by cation exchange chromatography, hydrophobic layer chromatography and molecular sieve chromatography in a combined mode. Recombinant human serum albumin-interferon alpha 2b fusion protein produced meets the regulations of Chinese Pharmacopoeia. The present invention has the advantages of high fermentation expression rate, reasonable purification technical combination, simplified operation procedure, high yield and low production cost. The present invention meets industrial requirements.