Malachite green molecularly imprinted solid phase extraction filler and preparation method thereof

A malachite green, molecular imprinting technology, applied in chemical instruments and methods, other chemical processes, etc., to achieve the effects of good chemical stability, uniform particle size, and easy to enlarge industrialization

Active Publication Date: 2012-06-20
WUXI ZODOLABS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The existing technology has made some progress in the synthesis of molecularly imprinted solid phase extraction packing materials, but there is still a big gap in the practical application of these molecularly imprinted solid phase extraction packing materials. Therefore, it is necessary to improve the existing technology and explore a particle size The preparation method of uniform malachite green molecularly imprinted solid-phase extraction filler is a key problem to be solved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Add the organic phase composed of 80g styrene, 10g divinylbenzene, 50g malachite green and 3g benzoyl peroxide into the oil phase storage tank, and form a uniform oil phase fluid through stirring;

[0043] (2) Add 1000ml of distilled water and 10g of polyvinyl alcohol into the stirred polymerization reactor, stir at 80°C for 30 minutes, completely dissolve the polyvinyl alcohol, and keep the stirring speed at 200rpm;

[0044](3) Apply appropriate pressure to keep the pressure in the oil phase storage tank at 0.1 MPa, open the second regulating valve, and the oil phase fluid will disperse into the water phase in the polymerization reactor through the nozzle to form an O / W suspension at a constant temperature of 80°C React for 3 hours to obtain malachite green polystyrene composite microspheres;

[0045] (4) After cooling, the malachite green template molecules are fully washed with acetone, ethanol and water to obtain the malachite green molecularly imprinted polysty...

Embodiment 2

[0047] (1) Pour the organic phase composed of 70g methyl methacrylate, 5g ethylene glycol dimethacrylate, 50g malachite green and 4g benzoyl peroxide into the oil phase storage tank, and form a uniform mixture after stirring oil phase fluid;

[0048] (2) Add 1000ml of distilled water and 15g of polyvinylpyrrolidone into the stirred polymerization reactor, and stir at 80°C for 30 minutes to completely dissolve the polyvinylpyrrolidone, and keep the stirring speed at 200rpm;

[0049] (3) Apply appropriate pressure to keep the pressure in the oil phase storage tank at 0.1 MPa, open the second regulating valve, and the oil phase fluid will disperse into the water phase in the polymerization reactor through the nozzle to form an O / W suspension at a constant temperature of 80°C After reacting for 3 hours, malachite green polymethyl methacrylate composite microspheres were obtained;

[0050] (4) After cooling, the malachite green template molecules are fully washed with acetone, eth...

Embodiment 3

[0052] (1) The organic phase that 70g vinyl acetate, 4g divinylbenzene, 50g malachite green and 4g benzoyl peroxide is formed, adds in the oil phase storage tank, forms uniform oil phase fluid through stirring;

[0053] (2) Add 1000ml of distilled water and 15g of polyvinyl alcohol into the stirring polymerization reactor, and stir at 80°C for 30 minutes to completely dissolve the polyvinyl alcohol, and keep the stirring speed at 200rpm;

[0054] (3) Apply appropriate pressure to keep the pressure in the oil phase storage tank at 0.1 MPa, open the second regulating valve, and the oil phase fluid will disperse into the water phase in the polymerization reactor through the nozzle to form an O / W suspension at a constant temperature of 80°C React for 3h to obtain malachite green polyvinyl acetate composite microspheres;

[0055] (4) After cooling, the malachite green template molecules are fully washed with acetone, ethanol and water to obtain the malachite green molecularly impri...

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PUM

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Abstract

The invention relates to a malachite green molecularly imprinted solid phase extraction filler and a preparation method thereof. The preparation method comprises the following steps of: fully mixing a hydrophobic alkene monomer, malachite green and an oil-soluble initiator to form an oil-phase fluid which is uniformly distributed; and smashing and dispersing the oil-phase fluid into oil drops of uniform sizes with a jet suspension polymerization method, forming a uniform O/W suspension in a water phase, polymerizing at a constant temperature to obtain malachite green polymer compound microspheres, and removing malachite green template molecules to obtain the solid phase extraction filler. The solid phase extraction filler prepared with the method has uniform particle diameter, stable chemical property and high selectivity and high yield when used for detecting the residues of malachite green in foods.

Description

technical field [0001] The invention belongs to the field of food safety testing, and in particular relates to a malachite green molecularly imprinted solid-phase extraction filler and a preparation method. Background technique [0002] Malachite Green (Malachite Green, MG), also known as basic green, base block green, malachite green, is a triphenylmethane dye, and is widely used to prevent and treat saprolegniasis, gill mold and Small melon worm disease, etc. However, malachite green and its metabolites are prone to high residues in aquatic products, and it is easy to cause carcinogenic, teratogenic and mutagenic phenomena after human consumption. [0003] In the detection of food safety residues, the pretreatment process of samples is very important. Solid-phase extraction (Solid-Phase Extraction, SPE) is an efficient and rapid sample preparation technology developed in recent years. The trace components in the sample are enriched, concentrated and purified according to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F212/08C08F212/36C08F2/20C08F2/01C08F220/14C08F222/14C08F218/08C08F220/32C08J9/26B01J20/285B01J20/30
Inventor 阳承利
Owner WUXI ZODOLABS BIOTECH
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