Compound traditional Chinese medicine for effectively preventing and controlling H1N1 and H3N2 swine influenza and porcine reproductive and respiratory syndrome, and preparation method thereof

A technology for porcine blue-ear disease and swine flu, which is applied in medical preparations, pharmaceutical formulations, antiviral agents and other directions containing active ingredients, can solve the problems that have not been researched and cannot fully evaluate the effect of porcine reproductive and respiratory syndrome virus.

Inactive Publication Date: 2016-12-21
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Looking at the current experimental research on the anti-virus of traditional Chinese medicine compounds, there are many studies on single prescriptions against swine influenza virus and porcine reproductive and respiratory syndrome virus (or "porcine blue ear disease"). At present, no compound prescription has been developed that can simultaneously Anti-swine influenza virus and porcine reproductive and respiratory syndrome virus
Moreover, most of them are independently studied by in vivo test or in vitro test, which cannot comprehensively evaluate the effect of traditional Chinese medicine compound against swine influenza virus and porcine reproductive and respiratory syndrome virus.

Method used

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  • Compound traditional Chinese medicine for effectively preventing and controlling H1N1 and H3N2 swine influenza and porcine reproductive and respiratory syndrome, and preparation method thereof
  • Compound traditional Chinese medicine for effectively preventing and controlling H1N1 and H3N2 swine influenza and porcine reproductive and respiratory syndrome, and preparation method thereof
  • Compound traditional Chinese medicine for effectively preventing and controlling H1N1 and H3N2 swine influenza and porcine reproductive and respiratory syndrome, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 In vitro anti-porcine reproductive and respiratory syndrome virus effect of Chinese medicine compound preparation

[0046] 1. Determination of the median lethal dose (TCID50) of PRRSV on MARC-145 cells

[0047] The virus to be tested was serially diluted 10 times in 10 concentration gradients, and the virus solution was added to a 96-well plate full of Marc-145 cells at a dose of 100 μl / well, and each gradient was repeated 8 times, and the last 2 columns were For the cell control group, the virus liquid was discarded after adsorption at 37°C for 1.5 h, and 100 μl of maintenance liquid was added, and cultured in a CO2 cell incubator.

[0048]The cell lesion was observed every day, and the OD value of each well was measured by the MTT method after the lesion reached 75%. Growth inhibition rate of virus on cells = (OD value of cell control group - OD value of virus control group) / OD value of cell control group. The median toxic concentration (TCID50) of the v...

Embodiment 2

[0068] Example 2 Prevention and treatment effect of traditional Chinese medicine compound on mice infected with H1N1 and H3N2 swine influenza virus

[0069] 1. H1N1 and H3N2 swine influenza virus half infectious dose to chicken embryo (EID 50 ) Determination

[0070] The virus liquid was sequentially diluted 10 times, and inoculated in the allantoic cavity of 10-day-old chicken embryos, each dilution was inoculated with 0.2ml / piece, and 4 replicates were made, and normal chicken embryos were set as a control at the same time. Place them in an incubator at 37°C for 72 hours, discard the dead embryos within 24 hours, harvest the allantoic fluid of the dead embryos after 24 hours and live embryos after 72 hours, and measure the titer of HA. Calculate EID according to the Reed-Muench method 50 .

[0071] . Effects of traditional Chinese medicine compound on pneumonia in mice infected with H1N1 and H3N2 swine influenza virus

[0072] (1) Grouping of experimental animals

[00...

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Abstract

The present invention discloses a compound traditional Chinese medicine for effectively preventing and controlling H1N1 and H3N2 swine influenza and porcine reproductive and respiratory syndrome, wherein the types and the contents of the traditional Chinese medicines in the compound traditional Chinese medicine are provided. The compound traditional Chinese medicine comprises, by weight, 20 parts of ephedra sinica stapf, 20 parts of scutellaria baicalensis, 9 parts of rhizoma anemarrhenae, 9 parts of bulbus fritillariae cirrhosae, 9 parts of apricot kernel, 9 parts of herba houttuyniae, 9 parts of morus alba l, 9 parts of rheum palmatum l, and 6 parts of licorice root. The invention further provides a preparation method of the compound traditional Chinese medicine. The preparation method comprises: weighing the raw materials according to the weight part ratio to obtain 100 g of the compound traditional Chinese medicine, crushing to achieve 20 mesh, and soaking for 1 h; carrying out water decocting 2 times, wherein the boiling time is 30 min every time; merging the two decocting liquids; and carrying out normal pressure vacuum suction filtration and concentration, carrying out volume metering to achieve 100 mL of the water decocting liquid, carrying out high pressure sterilization on the water decocting liquid, and storing in a 4 DEG C refrigerator so as to be spare. According to the present invention, the in vivo and in vitro test results prove that the compound traditional Chinese medicine preparation can effectively prevent and control H1N1 and H3N2 swine influenza and porcine reproductive and respiratory syndrome.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine for veterinary use, and relates to a traditional Chinese medicine compound for effectively preventing and treating H1N1 and H3N2 swine flu and pig blue-ear disease and a preparation method thereof. Background technique [0002] Swine influenza (swine influenza, SI), also known as swine influenza, is an acute, febrile, highly contagious respiratory disease caused by swine influenza virus (swine influenza virus, SIV) of the family Orthomyxoviridae. Infectious diseases are clinically characterized by sudden onset, high fever, cough, dyspnea, recurrent attacks, high morbidity, and low mortality. The disease occurs all over the world, causing serious harm, huge economic losses, and a threat to human health. Its prominent epidemic feature is that it often exists in a subclinical form and often causes secondary or mixed infections of other respiratory bacteria and viruses, such as porcine rep...

Claims

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Application Information

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IPC IPC(8): A61K36/8966A61P31/16A61P31/14
CPCA61K36/17A61K36/484A61K36/539A61K36/605A61K36/708A61K36/736A61K36/78A61K36/8964A61K36/8966A61K2236/331A61K2236/39A61K2300/00
Inventor 王丙云刘樱何永明冼琼珍计慧琴陈胜锋陈志胜张贝莹
Owner FOSHAN UNIVERSITY
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