Application of TREM-2 gene in identification of susceptibility of pigs to reproductive and respiratory syndrome virus

Abstract

The invention discloses an application of TREM-2 gene in identification of susceptibility of pigs to a reproductive and respiratory syndrome virus , and provides a group of TREM-2 expression level detection primer. The group of detection primers comprises an upstream primer TREM2-F represented by SEQ ID NO.1 and a downstream TREM2-R represented by SEQ ID NO.2. Researches show that the TREM-2 gene expression level in the pigs is related with PRRSV infection, and the TREM-2 can regulate the expression of CD163 in order to regulate duplication of the PRRSV; and the pigs can resist the PRRSV when the TREM-2 expression level is low, and the pigs are susceptible to the PRRSV when the TREM-2 expression level is high. The TREM-2 gene lays a solid foundation for identification of the susceptibility of pigs to the PRRSV and screening and cultivation of PRRSV-resistant pigs as a novel PRRSV susceptibility determination standard, and is of great significance to control the porcine reproductive and respiratory syndrome.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of TREM-2 gene in identifying susceptibility of pigs to PRRS virus. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), commonly known as blue ear disease, is caused by porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome virus, PRRSV), first appeared in the United States in 1987, and then in reports from Europe. my country first isolated PRRSV in 1996 by Guo Baoqing and others, and named the isolated strain CH-1a, which confirmed the existence of the disease in my country. As early as 1992, the World Organization for Animal Health (Office International Des Epizooties, OIE) listed the disease as a Class B infectious disease. Currently, PRRS is one of the most important infectious diseases in the swine industry, causing huge economic losses to the swine industry worldwid...

AI Technical Summary

Problems solved by technology

The difficulty of prevention and control of PRRSV is mainly manifested in the following aspects: (1) macrophage and immunosuppressive diseases, PRRSV mainly infects pig alveolar macrophages (Porcinealveolar macrophages, PAMs), and PAMs are immune cells that destroy PAMs, thereby Destroy the body's immune system, thereby causing immunosuppression; (2) antigenic variability, currently PRRSV mutates rapidly, and the use of attenuated vaccines is one of the reasons for the virus to mutate. Recently, it has been reported in the literature that a new strain of PRRSV, NADC30, has appeared in the United States. In China, a new strain similar to that of the United States was also isolated, named NADC30-like, and another literature reported that a PRRSV-causing virus strain with a high degree of homology to the vaccine virus genome was isolated from a pig farm, and its virulence was enhanced. Analysis may be possible (3) The vaccine has no cross-protection, and PRRSV vaccines on the market have almost no cross-protection, and there is no cross-protection between different strains; (4) Antibody dependence is enhanced, and the PRRSV Infection will stimulate the body to produce antibodies, but low-titer antibodies not only cannot neutralize the virus, but can promote the proliferation of the virus; (5) The virus is persistently infected. After PRRSV infection, the virus blood can be detected in pigs for a long time syndrome, PRRSV lasts up to 5 months in the body; (6) mixed infection, mixed infection of PRRSV and other diseases is common in clinical practice, especially circovirus, Haemophilus parasuis, porcine pneumonia, etc. and PRRSV The mixed infection of PRRSV makes the prevention and control of PRRSV even more difficult

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