Gas interval-type droplet evaporation-resistant micro fluidic chip apparatus and method thereof

A microfluidic chip and anti-evaporation technology, which is applied in the field of microfluidic analysis, can solve problems such as cross-contamination, and achieve the effects of inhibiting evaporation, preventing volume reduction, and avoiding material exchange

Active Publication Date: 2016-12-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are also some problems when the oil phase is in direct contact with the droplets: some fat-soluble substances inside the droplets may be dissolved in the oil phase separated by the water droplets, so that there will be cross-contamination between different droplets

Method used

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  • Gas interval-type droplet evaporation-resistant micro fluidic chip apparatus and method thereof
  • Gas interval-type droplet evaporation-resistant micro fluidic chip apparatus and method thereof
  • Gas interval-type droplet evaporation-resistant micro fluidic chip apparatus and method thereof

Examples

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Embodiment 1

[0069] Microfluidic droplet chip device that prevents droplet evaporation, see figure 1 , figure 1 A longitudinal sectional view of the device. The whole device is circular, placed in a transparent plastic petri dish with a diameter of 6 cm, and the bottom surface of the lower end of the chip device is in contact with the petri dish. The anti-evaporation cover plate 3 with a through hole for carrying the anti-evaporative phase 2 is made of an acrylic plate through laser engraving. 1.0 mm, the anti-evaporation phase is carried in the through hole.

[0070] The bottom surface of the petri dish is directly used as the droplet plate 6 carrying the microdroplet 1, and the thickness is 1.5 mm. There is a layer of annular spacer 5 between the perforated anti-evaporation cover plate 3 and the droplet plate 6, which is made of polydimethylsiloxane (PDMS) material and has a thickness of 3.0 mm, which is used to avoid the droplet 1 and anti-evaporation Phase 2 contacts. Epoxy resin ...

Embodiment 2

[0075] Conventional cell culture is carried out in culture flasks or culture dishes. Cells grow in the container according to their own survival characteristics. Adherent cells grow in a single layer on the surface, and suspension cells grow in suspension in the medium. Conventional cell culture in droplets is to wrap the droplets containing cells and their culture medium in the oil phase for cultivation. However, by using the present invention, the cells and their culture fluid can be cultured in droplets without contacting the anti-evaporation phase, so as to avoid possible interference and adverse effects of the anti-evaporation phase on cell culture.

[0076] Non-contact anti-evaporation microfluidic droplet chip device for studying cell droplet culture, see Figure 3a , 3b , 3c and 3d, Figure 3a , 3b , 3c and 3d are longitudinal cross-sectional views of the device. Figure 4a , 4b , 4c and 4d are partial enlarged views corresponding to the above four devices or long...

Embodiment 3

[0084] A microfluidic droplet chip device that can complete the pretreatment of trace protein samples, see Figure 4a , 4b Or 4c, which is a pretreatment device for a sample. Multi-sample, high-throughput pretreatment can be obtained by integrating the above independent devices Figure 3a , 3b or 3d.

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Abstract

The invention discloses a gas interval-type droplet evaporation-resistant micro fluidic chip apparatus. The micro fluidic chip apparatus comprises a control probe; a liquid drop plate used for bearing the micro liquid drop; an evaporation-resistant cover plate with an enclosed hood covered on a liquid drop bearing surface of the liquid drop plate, wherein a through hole structure used for avoiding a control probe when the liquid drop is generated is arranged on the position of the evaporation-resistant cover plate facing to a liquid drop zone; an enclosed space is formed between the evaporation-resistant cover plate and the liquid drop plate, wherein the enclosed space is filled with gas; after the micro liquid drop is generated, an isolation gap is generated between an evaporation-resistant phase and the micro liquid drop; and the evaporation-resistant phase is sealed in the through hole structure or the end face of the through hole structure. The evaporation-resistant phase does not directly contact with the micro liquid drop, by using a method for using gas to space the evaporation-resistant phase and the micro liquid drop in a formed local enclosed space, the cross contamination problem between the micro liquid drop and the evaporation-resistant phase as well as the micro liquid drop and the micro liquid drop can be completely avoided, and the evaporation-resistant and interval problems of the micro liquid drop can be solved.

Description

technical field [0001] The field of the invention relates to the field of microfluidic analysis, and in particular relates to a microfluidic chip device and method for preventing liquid droplet evaporation with gas intervals. Background technique [0002] Droplet microfluidics technology is a new technology and research direction with great development potential in the field of microfluidics in recent years. Simply put, droplet microfluidics is a technique for generating and manipulating tiny droplets in a network of microchannels. Different from a homogeneous continuous flow system, a droplet refers to a discontinuous fluid formed by separating one phase of liquid from another immiscible phase or multiple phases. Compared with the continuous flow microfluidic system, in the droplet microfluidic system, the droplets separated by the immiscible phase can be used as individual microreactors without dilution and cross-contamination during long-term reactions and transfers. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027B01L3/502792B01L2200/0642B01L2200/142B01L2300/0672B01L2300/0829B01L2300/0867B01L2400/02
Inventor 方群娄琦李紫艺
Owner ZHEJIANG UNIV
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