Method for removing No.2 broad bean wilt virus in chili seeds

A technology for seeds and peppers, applied in the fields of seed and rhizome treatment, biochemical equipment and methods, and microbial measurement/inspection. There are no problems such as the evaluation of the detoxification effect of pepper seeds, and the effects of fast detection efficiency, shortened detection time, and improved germination potential have been achieved.

Active Publication Date: 2017-01-04
XINJIANG AGRI UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the commonly used detection methods for the detoxification effect of seeds mainly include observation of symptoms at seedling stage, indicator plant method, ELISA, immunoelectron microscopy and RT-PCR technology. Although these methods can evaluate the detoxification effect, there are some stability Due to the disadvantages of poor sex, only qualitative detection, and limited sensitivity, it is impossible to accurately quantify the virus, and it is difficult to determi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for removing No.2 broad bean wilt virus in chili seeds
  • Method for removing No.2 broad bean wilt virus in chili seeds
  • Method for removing No.2 broad bean wilt virus in chili seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1. The detoxification effect comparison of several detoxification methods

[0037] In this embodiment, seven detoxification methods are designed to detoxify pepper seeds, and non-detoxification pepper seeds are used as a control. The amount of seeds for each treatment is about 300 plump and poisonous pepper seeds. After seed detoxification treatment, BBWV2 in pepper seeds was quantitatively detected by TaqMan probe real-time fluorescent quantitative PCR detection method, and the detoxification effect of each detoxification method was compared.

[0038] 1. The detoxification method is designed as follows:

[0039] (1) Dry heat treatment method: Put the chili seeds to be detoxified in an oven, heat them with hot air for 48 hours at 70 ℃, and then let them stand to cool.

[0040] (2) Moist heat treatment method: Soak the chili seeds to be detoxified in 5 times the volume of boiling water, and quickly oscillate and cool to 40-45 ℃, filter the aqueous solution an...

Embodiment 2

[0063] Embodiment 2. The impact of several detoxification methods on pepper seed germination rate and germination potential

[0064] Randomly select 150 capsicum seeds of each detoxification treatment and non-detoxification treatment in Example 1, divide them into 3 groups, adopt the culture dish filter paper constant temperature germination method after soaking in cold water for 4 h, and germinate the seeds in a constant temperature incubator at 28°C. For the germination test, observe and count the germination situation day by day from the 3rd day after the treatment, record the number of germinations, measure the germination potential with the number of germinations of each detoxification treatment on the 7th day, and end the germination test on the 10th day. Germination rate (%) = (number of germinated seeds / number of tested seeds) × 100; germination potential (%) = (number of seeds germinated within a specified time / number of tested seeds) × 100.

[0065] The results o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for removing No.2 broad bean wilt virus in chili seeds and belongs to the technical field of treatment. Specifically, the method comprises the following steps: (1) carrying out moisture-thermal treatment; (2) carrying out pesticide treatment; (3) filtering seeds; (4) poaching; (5) drying; (6) detecting viruses. According to the method provided by the invention, the disadvantages of the prior art are overcome; when the method provided by the invention is utilized, the germination rate and germinating potential of the chili seeds are improved, and the removal of the No.2 broad bean wilt virus in the chili seeds is more complete; the removal rate reaches 100 percent. Furthermore, the invention provides a TaqMan probe real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection method capable of evaluating a virus removing effect; the content of the viruses in the virus-removed seeds can be accurately grasped, so that technical indexes of passivated viruses are accurately controlled under the condition that high quality of the seeds is guaranteed, and the virus activity is inhibited to the greatest extent. The method for removing the viruses of the chili seeds, provided by the invention, is not limited by time and influenced by environment, and can be used in a laboratory or a basic unit all year round; the method is very practical and can be used for large-scale production of the virus-removed chili seeds.

Description

technical field [0001] The invention relates to the technical field of seed treatment, in particular to a method for removing broad bean wilt virus No. 2 from pepper seeds. Background technique [0002] Pepper is the second largest vegetable crop in my country, with an annual cultivated area of ​​about 1.33 million ha 2 . Virus disease is an important disease that endangers the production of peppers, resulting in a 20%-70% reduction in pepper production and poor quality, which has become an important obstacle restricting the development of the pepper industry. Broad bean wilt virus (BBWV) is a worldwide epidemic virus with a wide range of hosts, which can infect 328 species of plants belonging to 44 families and 186 genera. According to different serotypes, broad bean wilt virus is divided into two species, namely BBWV1 and BBWV2. The isolates obtained in my country are mainly BBWV2, and BBWV1 has not been found yet. The virus was first discovered in Australia. Since it w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01C1/00C12Q1/70C12Q1/68
CPCA01C1/00C12Q1/686C12Q1/6895C12Q1/701C12Q2561/113C12Q2563/107C12Q2545/114C12Q2561/101C12Q2531/113
Inventor 罗明韩剑张强赵冰梅张祥林徐金虹
Owner XINJIANG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap