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A three-dimensionally printed strontium-containing mesoporous bioglass scaffold loaded with soybean isoflavones and its preparation method

A technology of soybean isoflavones and bioglass, applied in the field of biomedical materials, to achieve the effects of treating osteoporosis, excellent in vivo osteogenic activity, and good biocompatibility

Active Publication Date: 2019-09-20
上海浩渤医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, there is no literature report on 3D printed strontium-containing mesoporous bioglass scaffolds loaded with soy isoflavones

Method used

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  • A three-dimensionally printed strontium-containing mesoporous bioglass scaffold loaded with soybean isoflavones and its preparation method
  • A three-dimensionally printed strontium-containing mesoporous bioglass scaffold loaded with soybean isoflavones and its preparation method
  • A three-dimensionally printed strontium-containing mesoporous bioglass scaffold loaded with soybean isoflavones and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1: Preparation of a three-dimensionally printed strontium-containing mesoporous bioglass scaffold loaded with soybean isoflavones

[0038] A method for preparing a three-dimensionally printed strontium-containing mesoporous bioglass support loaded with soybean isoflavones according to the present invention comprises the following steps:

[0039] Step 1, preparing strontium-containing bioactive glass powder, passing through a 400-mesh sieve to make its size smaller than 37 μm, dissolving soybean isoflavones in phosphate buffer saline (phosphate buffer saline, PBS) to obtain 100 mg / ml soybean isoflavones solution, soak strontium-containing mesoporous bioglass powder in a PBS solution containing 100 mg / ml for 24 hours, load soybean isoflavones into the mesoporous pores of Sr-MBG; dry and store the powder for later use;

[0040] For the preparation method of strontium-containing bioactive glass powder, please refer to the literature Zhang J, Zhao S, Zhu Y, et al.Thr...

Embodiment 2

[0045] Example 2: Adhesion of MC3T3-E1 cells on a three-dimensionally printed strontium-containing mesoporous bioglass scaffold loaded with soybean isoflavones

[0046] MC3T3-E1 cells (mouse embryonic osteoblast precursor cells) were purchased from the Cell Bank of the Chinese Academy of Sciences.

[0047] Three-dimensionally printed strontium-containing mesoporous bioglass scaffold material samples were soaked in 75% medical alcohol for 4 hours, and then sterilized under ultraviolet light for 24 hours. At the same time, 1ml of α-MEM containing 10% FBS was added to the 24-well plate for culture The substrate was pre-wetted for 24 hours, the medium was replaced, and then the sterilized scaffold material was transferred into a 24-well plate. Then, will contain 1×10 5 200 μl of culture medium of MC3T3-E1 cells was added dropwise to each sample, and incubated at 37°C, 5% CO 2 Atmosphere CO 2 Cultivate in an incubator, and after 8 hours the cells basically adhere to the wall, ad...

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Abstract

The invention discloses a three-dimensional printing strontium-containing mesoporous bioglass bracket loaded with soy isoflavone, and belongs to the field of the biomedical material. The bracket is composed of the strontium-containing mesoporous bioglass (Sr-MBG), polycaprolactone (PCL) and soy isoflavone, wherein the quality proportions of the Sr-MBG and PCL are 70% and 30% respectively, and 5% of Ca in the Sr-MBG is replaced by Sr. The soy isoflavone is loaded into the mesoporous of the Sr-MBG, two materials of the Sr-MBG loaded with the soy isoflavone and the PCL are printed to be the cubical biological activity bracket layer by layer by using the three-dimensional printing technology, each layer is formed by crisscrossed perpendicular lines, and the pore formed by the perpendicular lines is a square. The bracket can be applied to the repair and treatment in allusion to the bone defect, has good biocompatibility and better capacity for promoting osteogenesis and can be absorbed and degraded by the living organism. The treatment effect for the bone defect of the osteoporosis patient can be enhanced after the soy isoflavone is loaded, and the bracket is the perfect bracket material for bone tissue engineering.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a three-dimensionally printed strontium-containing mesoporous bioglass support loaded with soybean isoflavones. Background technique [0002] According to incomplete statistics, there are more than 7 million patients in the world who need to use bone repair materials for bone defects caused by various reasons every year. Currently, commonly used bone repair materials include autologous bone, allogeneic bone, hydroxyapatite, and bioactive ceramics. , biological glass, inert materials, etc. As bone repair materials, autologous bone and allogeneic bone have shortcomings such as insufficient supply, infection, and possible disease transmission, while hydroxyapatite and inert materials have disadvantages such as lack of bioactivity and non-degradability, so in recent years, biodegradable bone implants Injection materials have become a research hotspot of bone repair materials. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/58A61L27/56A61L27/54A61L27/10A61L27/18B33Y80/00B33Y70/00
Inventor 禹宝庆朱钰方张旭裴鹏
Owner 上海浩渤医疗科技有限公司
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