A method and application for simultaneously detecting inorganic anions, organic acids and three phytochemicals in plants using a valve switching method
A technology of inorganic anions and organic acids, applied in the field of analytical chemistry, can solve the problems of unsatisfactory detection timeliness, waste of human and financial resources, etc., and achieve the effects of low detection limit, good precision and good reproducibility
Active Publication Date: 2019-03-12
ZHEJIANG SHUREN UNIV
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Problems solved by technology
If the method of separate measurement is still used, it will not only cause a waste of human and financial resources, but also cannot satisfy some applications with strong detection timeliness.
At present, there is no effective and reliable method that can simultaneously determine the content of the above substances, so it is urgent to develop a method that can simultaneously detect inorganic anions, organic acids and various phytochemicals in plants
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[0023] The present invention will be further described below in conjunction with the accompanying drawings and examples of implementation.
[0024] Chromatographic analysis of inorganic anions, organic acids and phytochemicals, the specific steps are as follows:
[0025] 1. Instruments and reagents
[0026] The structure of the valve switching device based on the method of the present invention is as follows: figure 1 As shown, it includes pump, six-way valve 1, Elliott HypersilODS analytical column (C18 column), six-way valve 2, enrichment column, guard column, analytical column, suppressor, conductivity detector, and UV detector. The six-way valve 1 injects samples through the quantitative loop, and is connected to the six-way valve 2 through the Elite Hypersil ODS analytical column. The enrichment column is placed in the six-way valve 2. Connect with guard column, analytical column, suppressor and conductivity detector in turn. The waste liquid outlets of each detection ...
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Abstract
The invention discloses a method of utilizing valve switching method to simultaneously detect inorganic anions, organic acid and three phytochemicals in plant and application of the method. The method includes that a sample is brought into a C18 column connected with high performance liquid chromatograph by leacheate; due to the fact that the organic acid and the anions do not stay in the C18 column, by setting proper valve switching time, the organic acid and the anions are all switched into an ion chromatography column; the three phytochemicals staying in the C18 column continue being flushed out of the chromatography column along with leaching of the leacheate to be detected by an ultraviolet detector; the anions and the organic acid entering the ion chromatography column are flushed out of the same along with leaching of alkaline leacheate to be detected by a conductivity detector. Simultaneous detection of the inorganic ions, the organic acid and the three phytochemicals can be realized by connecting the high performance liquid chromatograph and an ion chromatograph through valve switching. The method is ideal in overall stability and reproducibility and accurate and reliable in measured data and can be widely applied in industries of food production and biomedicine needing active ingredient detection.
Description
technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method and application for simultaneously detecting inorganic anions, organic acids and three plant elements in plants by using a valve switching method. Background technique [0002] At present, high performance anion exchange chromatography (ion chromatography) is usually used to detect inorganic anions and organic acids in plants, while phytochemicals such as scopolamine, rutin, and quercetin are detected by reversed-phase high performance liquid chromatography, spectrophotometry, etc. method for separate measurement. However, in analytical tests, it is often necessary to simultaneously determine inorganic anions, organic acids and different phytochemicals. If the method of separate and separate measurement is still used, it will not only cause a waste of human and financial resources, but also cannot satisfy some applications with strong timeliness for detec...
Claims
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IPC IPC(8): G01N30/02G01N30/08G01N30/20G01N30/96
CPCG01N30/02G01N30/08G01N30/20G01N30/96G01N2030/201
Inventor 陈梅兰吴琪琪
Owner ZHEJIANG SHUREN UNIV