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Method for rapidly detecting mulberry anthocyanins and flavonoids by utilizing UPLC-TUV

A technology of anthocyanin and mulberry, which is used in measuring devices, instruments, scientific instruments, etc., to achieve the effect of good repeatability and high degree of separation

Active Publication Date: 2017-01-04
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the simultaneous separation and rapid detection of mulberry anthocyanins and various flavonoids by ultra-high liquid chromatography

Method used

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  • Method for rapidly detecting mulberry anthocyanins and flavonoids by utilizing UPLC-TUV
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  • Method for rapidly detecting mulberry anthocyanins and flavonoids by utilizing UPLC-TUV

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Embodiment 1

[0027] 1. Instruments and reagents

[0028] 1.1 Instrument

[0029] DHG-9140A electric heating constant temperature blast drying oven, Shanghai Qixin Scientific Instrument Co., Ltd.; FA2004B electronic balance, Shanghai Jingtian Electronic Instrument Co., Ltd.; GX-03 150g functional pulverizer, Zhejiang Gaoxin Industry and Trade Co., Ltd.; KQ-500DV CNC Ultrasonic cleaner, Kunshan Ultrasonic Instrument Co., Ltd.; CT15RE refrigerated centrifuge, Hitachi, Japan; Milli-Q system, Millipore, USA; Acquity UPLC I-Class system, Waters, USA.

[0030] 1.2 Reagents

[0031] cyanidin-3-O-glucoside (>98%), cyanidin-3-O-rutinoside (>98%), pelargonidin-3-O-glucoside (>98%), chromatographic Grade ammonium formate, ammonium acetate, formic acid, and acetic acid were purchased from Sigma; rutin (>98%), isoquercetin (>98%), morin (>98%), quercetin (>98%) , kaempferol (>98%) were purchased from Chengdu Chroma Biotechnology Co., Ltd.; chromatographic grade acetonitrile and methanol were purchase...

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Abstract

The invention discloses a method for rapidly detecting mulberry anthocyanins and flavonoids by utilizing UPLC-TUV, detecting conditions for ultra high performance liquid chromatography detection and a sample treating method. According to the method, three types of anthocyanins and five types of flavonoids in mulberries can be rapidly separated and detected at a certain flow velocity and in a proportional gradient elution mode. The method is sensitive, high in separating degree and good in repeatability.

Description

technical field [0001] The invention belongs to the technical field of detection of mulberry anthocyanins and flavonoids, and in particular relates to a method for rapidly detecting mulberry anthocyanins and flavonoids by using UPLC-TUV. Background technique [0002] Mulberry is the fruit of the mulberry tree when it is mature. It contains a variety of nutrients and medicinal ingredients. As a traditional Chinese medicine, it has the functions of nourishing blood and nourishing yin, promoting body fluid and moistening dryness. Thirst, blood deficiency, constipation, etc. Modern phytochemical studies have confirmed that mulberry contains various active ingredients such as alkaloids, flavonoids, polyphenols, and polysaccharides, and has pharmacological effects such as hypoglycemic, antioxidative, and antitumor. [0003] Mulberry contains extremely rich anthocyanins and flavonoids, both of which are polyhydroxy compounds, so they have good biological activity. Studies have fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 何宁佳陈虎向仲怀
Owner SOUTHWEST UNIV