A method for the determination of six aromatic amine compounds in mainstream cigarette smoke using online spe/lc-ms/ms

A technology of cigarette mainstream smoke and aromatic amines, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome and time-consuming processing, human errors, etc., and achieve the effects of reducing sample analysis interference, high precision, and reducing pollution

Active Publication Date: 2018-05-18
中国烟草总公司山东省公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide an online solid-phase extraction-liquid chromatography-tandem mass spectrometry technology for the determination of the content of the main stream smoke of cigarettes in view of the shortcomings of tedious and time-consuming sample pretreatment and easy introduction of human error in the existing aromatic amine compound detection technology. 6 Methods of Aromatic Amines

Method used

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  • A method for the determination of six aromatic amine compounds in mainstream cigarette smoke using online spe/lc-ms/ms
  • A method for the determination of six aromatic amine compounds in mainstream cigarette smoke using online spe/lc-ms/ms
  • A method for the determination of six aromatic amine compounds in mainstream cigarette smoke using online spe/lc-ms/ms

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Experimental program
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Effect test

Embodiment 1

[0032] 1. Sample Preparation

[0033] Smoke the balanced and screened cigarettes according to the standard conditions stipulated in ISO 3308:2000, and use 4 Cambridge filters (φ44mm) to capture the particulate matter in the mainstream smoke of 20 cigarettes (each Cambridge filter captures 5 Particle phase in the mainstream smoke of cigarettes); the absorption bottle pre-filled with 25mL 2% formic acid solution was connected to the back of the trap through a hose to collect the gas phase.

[0034] Remove the absorption bottle for collecting the gas phase, add 0.25mL mixed internal standard (d 9 -o-toluidine, d 3 -o-anisidine, d 6 -2,6-Dimethylaniline, d 7 -1-aminonaphthalene and d 9 -4-aminobiphenyl), mix well, and directly enter the Online SPE / LC-MS / MS analysis.

[0035] Fold the Cambridge filter that has captured the particulate matter in the mainstream smoke of 20 cigarettes into a 250mL ground-mouth conical flask, add 1.0mL mixed internal standard and 100mL 2% formic a...

Embodiment 2

[0070] The Kentucky reference cigarette 3R4F was smoked according to the standard conditions stipulated in ISO 3308:2000. The particulate matter of 5 cigarettes was collected by each Cambridge filter, and the gas phase was collected in an absorption bottle containing 25mL of 2% formic acid solution.

[0071] Then according to the steps and conditions described in Example 1, sample processing and analysis were carried out. 6 parallel experiments were carried out and the determination was divided into 5 days. The relative standard deviations of the determination of each compound were respectively between 2.50-5.52% and 2.24-6.69, indicating that the method has good reproducibility and stability.

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Abstract

The invention discloses a method for determining six aromatic amine compounds in cigarette mainstream smoke by using online SPE / LC-MS / MS. It uses the Cambridge filter and an absorption bottle containing 2% formic acid solution to capture the aromatic amine compounds in the main cigarette smoke particle and gas phase matters, respectively, ultrasonically extracting the filter by 2% formic acid solution, adding interior labels into the two solutions and determining the six aromatic amine compounds by online SPE / LC-MS / MS, including o-toluidine, 2,6-dimethylaniline, o-anisidine, 1-aminonaphthalene, 2-aminonaphthalene and 4-aminobiphenyl. The method has the advantages of simplifying the sample pretreatment process and extracting with the formic acid solution to reduce the pollution. The two-dimensional in-line solid-phase extraction composed of the cationic column and the reversed-phase C18 column is used to purify the sample to be tested and the purification effect is good; each target is quantitatively analyzed using their specific internal standard, effectively reducing the matrix effect.

Description

technical field [0001] The invention belongs to the technical field of analysis and testing of components related to cigarette smoke releases, and specifically relates to a method for measuring six kinds of aromatic amine compounds in mainstream cigarette smoke by using online solid-phase extraction-liquid chromatography-tandem mass spectrometry, including orthoformazine Aniline, 2,6-dimethylaniline, o-anisidine, 1-aminonaphthalene, 2-aminonaphthalene and 4-aminobiphenyl. Background technique [0002] As important raw materials and intermediates of organic synthesis, aromatic amines are widely used in the industrial production of medicines, insecticides, azo dyes, plastics, and rubber. In addition, people have detected various aromatic amine components from various environmental substances such as air, discharged wastewater, food additives, cosmetics, etc. Aromatic amine compounds can enter the human body through inhalation, ingestion, or skin contact, and can cause damage ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 纪立顺别振英朱友陈玉松蔚亦沛陆伟罗旭刘敏
Owner 中国烟草总公司山东省公司
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