Method and color card for rapid detection of nitrate in food and kit for detecting nitrate in food

A technology for detection reagents and detection methods, which is used in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of large consumption of reagents, cumbersome operation, and inability to detect nitrate samples. and other problems, to achieve the effect of less reagent consumption and reduced workload

Inactive Publication Date: 2017-01-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method must be operated in a laboratory, and the operation is cumbersome and time-consuming; cadmium can volatilize and has great toxicity to the human body
The reagents used in the enzymatic method are not easy to store and are not suitable for on-site detection operations outside the laboratory
The instruments used in chromatography are expensive, and the consumption of reagents is large, and professional testing personnel

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The nitrate detection reagent is a powder reagent mixed with citric acid, anhydrous sodium sulfate and naphthaleneethylenediamine hydrochloride, and the mass ratio of citric acid, anhydrous sodium sulfate and naphthaleneethylenediamine hydrochloride is 9:10:3.

Embodiment 2

[0039] Embodiment two, make nitrate detection reagent

[0040] The nitrate detection reagent also includes manganese sulfate monohydrate, p-aminobenzenesulfonic acid and zinc powder, and the mass ratio of each component is as follows:

[0041] Citric acid: 9 parts

[0042] Anhydrous sodium sulfate: 10 parts

[0043] Manganese sulfate monohydrate: 2.5 parts

[0044] 4-aminobenzenesulfonic acid: 1 part

[0045] Naphthaleneethylenediamine hydrochloride: 0.6 parts

[0046] Zinc powder: 3 parts.

Embodiment 3

[0047] Embodiment 3, making a standard color card

[0048] Prepare multiple standard solutions, each standard solution is an aqueous solution of sodium nitrate, and the concentration of sodium nitrate is 0mg, 2.5mg, 5mg, 10mg, 20mg, 30mg, 40mg, 50mg, 100mg of sodium nitrate in each kg solution; Put 1ml of standard solution in a 10ml colorimetric tube, add an ear spoon (about 0.025g) of nitrate detection reagent to each standard solution, mix well and let it stand for 1 minute to obtain the standard display of known nitrate concentration. color solution.

[0049] The color of each standard chromogenic solution is processed into a standard color card by computer processing. The content of nitrate corresponding to each color on the color card is 0mg / kg, 25mg / kg, 50mg / kg, 100mg / kg, 200mg / kg, 300mg / kg, 400mg / kg, 500mg / kg, 1000mg / kg.

[0050] After the above standard color cards are made, they can be used for a long time.

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PUM

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Abstract

The invention provides a method for quickly detecting nitrates in food, the detection steps are as follows: 1) prepare sodium nitrate aqueous solution whose concentration changes successively, as a standard solution, add citric acid, anhydrous sodium sulfate, naphthaleneethylenediamine hydrochloride mixed 2) Add pure water to the sample, shake and extract to obtain the sample treatment solution; 3) Take the above sample treatment solution , add the powder reagent mixed by citric acid, anhydrous sodium sulfate, naphthalene ethylenediamine hydrochloride etc. in a certain proportion, leave standstill after mixing, obtain the sample color developing solution with color; 4) compare the color of the sample color developing solution with The colors of each standard chromogenic solution are compared to obtain the nitrate content in the tested sample. The invention can quickly and easily detect the content of nitrate in the food, has high sensitivity, and is suitable for rapid on-site determination of non-professionals.

Description

technical field [0001] The invention relates to the technical field of food analysis and testing, in particular to a color card and kit for rapid detection of nitrate in food based on chemical reaction. Background technique [0002] In modern food safety testing, the significance of nitrate detection is very important. Nitrate itself is not harmful, but it will be reduced to nitrite under the action of microorganisms in the human body. Under certain conditions, nitrite and protein can form strong carcinogens - nitroso compounds. Seriously endanger human health. Studies have shown that 80% of the nitrate in the human body comes from vegetables, and in my country, a large amount, excessive or unreasonable use of chemical fertilizers, especially nitrogen fertilizers, on crops every year has caused serious nitrate pollution in fruits and vegetables, especially leafy vegetables , which has caused serious harm to my country's food industry. Research and development of new rapid,...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 沈建福陈钦保
Owner ZHEJIANG UNIV
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