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A kind of pear young fruit total phenol extraction method

An extraction method, a technology of total phenols, applied in the field of extraction of effective components in fruits, can solve the problems of young fruit abandonment, waste of polyphenolic biological resources, etc., and achieve the effect of improving the extraction rate

Active Publication Date: 2019-06-11
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In production, due to the needs of cultivation management such as physiological fruit drop or flower thinning and fruit thinning, a large number of young fruits are abandoned, resulting in a waste of polyphenolic biological resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] According to one embodiment of the present invention, a kind of pear young fruit total phenol extraction method comprises the following steps:

[0020] (1) Take the young pear fruit about 20 days after flowering as the material, cut the young pear fruit into thin strips, quickly freeze it with liquid nitrogen, and then pulverize it with a pulverizer.

[0021] (2) Take 5 grams of pulverized pulp, add ethyl acetate as the first extraction solvent at a ratio of 1:30 according to the ratio of material to liquid (i.e. the weight ratio of pulp to extraction solvent), and after ultrasonication, filtration, and rotary evaporation Finally, the first extract was obtained; wherein, the ultrasonic temperature was 40° C., the ultrasonic time was 10 min, and the ultrasonic power was 150 W.

[0022] Preferably, in this step (2), the duty cycle of the ultrasound is 0.6-0.8.

[0023] Preferably, in this step (2), the air pressure during ultrasonic operation is 6-8Mpa.

[0024] (3) Use...

Embodiment 2

[0029] According to one embodiment of the present invention, a kind of pear young fruit total phenol extraction method comprises the following steps:

[0030] (1) Take the young pear fruit about 20 days after flowering as the material, cut the young pear fruit into thin strips, quickly freeze it with liquid nitrogen, and then pulverize it with a pulverizer.

[0031] (2) Take 5 grams of pulverized pulp, add ethyl acetate as the first extraction solvent at a ratio of 1:20 according to the ratio of material to liquid (i.e. the weight ratio of pulp to extraction solvent), after ultrasonication, filtration, and rotary steaming , to obtain the first extract; wherein, the ultrasonic temperature is 30°C, the ultrasonic time is 20min, and the ultrasonic power is 150W;

[0032] Preferably, in this step (2), the duty cycle of the ultrasound is 0.6-0.8.

[0033] Preferably, in this step (2), the air pressure during ultrasonic operation is 6-8Mpa.

[0034] (3) Use the filter residue in s...

Embodiment 3

[0039] According to one embodiment of the present invention, a kind of pear young fruit total phenol extraction method comprises the following steps:

[0040] (1) Take the young pear fruit about 20 days after flowering as the material, cut the young pear fruit into thin pieces, freeze it quickly with liquid nitrogen, and then pulverize it with a pulverizer.

[0041] (2) Weigh 5 grams of pulverized pulp, add ethyl acetate as the first extraction solvent at a ratio of 1:10 according to the ratio of material to liquid (i.e. the weight ratio of pulp to extraction solvent), after ultrasonication, filtration, and rotary steaming , to obtain the first extract; wherein, the ultrasonic temperature is 50°C, the ultrasonic time is 30min, and the ultrasonic power is 150W.

[0042] Preferably, in this step (2), the duty cycle of the ultrasound is 0.6-0.8.

[0043] Preferably, in this step (2), the air pressure during ultrasonic operation is 6-8Mpa.

[0044] (3) Use the filter residue in ...

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Abstract

The invention relates to a young pear fruit total phenol extracting method. The young pear fruit total phenol extracting method comprises the following steps: cutting young pear fruit into thin strips, freezing the thin strips by using liquid nitrogen, and then crushing; weighing 5 g of the crushed pulp, and adding a first extracting solvent according to the material-to-liquid ratio of 1:30; performing ultrasonic treatment, filtering and rotary evaporating to obtain a first extract, wherein the ultrasonic treatment temperature is 40 DEG C, the ultrasonic treatment time is 10 minutes, and the ultrasonic treatment power is 150 W; adding a second extracting solvent into first filter residues according to the material-to-liquid ratio of 1:25, performing ultrasonic treatment, filtering and rotary evaporating, and filling the extracts which are obtained after the two-time rotary evaporating to the volume of 10 ml with pure methyl alcohol to obtain a final total phenol extracting solution, wherein the ultrasonic treatment temperature is 35 DEG C, the ultrasonic treatment time is 20 minutes, and the ultrasonic power is 150 W; adding 1 ml of an FC colour developing agent and 3 ml of 7.5 percent sodium carbonate solution into 1 ml of the total phenol extracting solution in sequence; shaking uniformly; measuring the absorbance under the wavelength of 765 nm after the colour is developed for 30 minutes.

Description

technical field [0001] The invention relates to a technology for extracting effective components in fruits, and more specifically, relates to a method for extracting total phenols from young pear fruits. Background technique [0002] Plant / fruit polyphenols are important secondary metabolites widely present in plants / fruits, and have various physiological functions such as anti-oxidation, anti-tumor, and liver protection. The content of plant / fruit polyphenols is less in grains and vegetables, but higher in the cortex of fruits and grains. [0003] Pear is the third largest fruit in my country. It contains a variety of polyphenols such as arbutin, chlorogenic acid, and vanillin, and has certain antioxidant and health functions. Studies have shown that the relative concentration of these polyphenols in mature fruit is not high, but the content in young pear fruit is very rich. [0004] In production, due to the needs of cultivation and management such as physiological fruit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/73A61P39/06
CPCA61K36/73A61K2236/33
Inventor 苏艳丽李秀根杨健王龙王苏珂薛华柏
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI