A method for the synergistic promotion of biological reduction of nitrobenzene by clay minerals and humic acid

A clay mineral and nitrobenzene technology, applied in the field of pollutant biological treatment, can solve the problems of low recycling rate, easy loss of enhanced mediator, low efficiency of nitrobenzene wastewater, etc., to achieve enhanced biological treatment and good reusability , The effect of facilitating the promotion and application

Inactive Publication Date: 2019-04-23
DALIAN UNIV OF TECH
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  • Summary
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  • Claims
  • Application Information

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Problems solved by technology

[0008] In order to solve the low efficiency of biological treatment of nitrobenzene wastewater and the 2+ In order to avoid problems such as easy loss of strengthening media and low recycling rate, the present invention provides a method for synergistically promoting the biological reduction of nitrobenzene by using clay minerals and humic acid

Method used

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  • A method for the synergistic promotion of biological reduction of nitrobenzene by clay minerals and humic acid
  • A method for the synergistic promotion of biological reduction of nitrobenzene by clay minerals and humic acid
  • A method for the synergistic promotion of biological reduction of nitrobenzene by clay minerals and humic acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Clay mineral NAu-2 and humic acid ESHA synergistically promote microbial reduction of nitrobenzene:

[0027] (1) Cultivation of microorganisms: S. oneidensis MR-1 was cultivated in soybean medium as a strain for reducing nitrobenzene. Autoclave the medium solution at 121 °C. S. oneidensis MR-1 was inoculated into soybean medium in a sterile operating bench at an inoculation ratio of 1:100; the inoculated medium was cultured in a constant temperature shaker at 30°C and 150 rpm for 24 hours to obtain the end of logarithmic growth phase. The S. oneidensis MR-1 bacteria solution is ready for use.

[0028] (2) Prepare a 10mmol / L PIPES buffer solution, add 10mmol / L sodium lactate, and transfer it to a 50mL serum bottle, while adjusting the pH to 6.8-7.2. Nitrogen aeration exhausts the oxygen in the headspace of the serum bottle and the aqueous solution, and puts it into an anaerobic box to ensure the anaerobic state.

[0029] (3) In the anaerobic box, add 0.03 g of nitrobe...

Embodiment 2

[0037] Utilizing clay minerals and humic acid to synergistically promote the recycling application of bioreduction of nitrobenzene.

[0038] (1) Prepare a 10mmol / L PIPES buffer solution, add 10mmol / L sodium lactate, transfer it to a 50mL serum bottle, and adjust the pH to 6.8-7.2. Nitrogen aeration exhausts the oxygen in the headspace of the serum bottle and the aqueous solution, and puts it into an anaerobic box to ensure the anaerobic state.

[0039] (2) In the anaerobic box, use a pipette to draw 3 mL of nitrobenzene stock solution (see Example 1 for the preparation method) and add it to the serum bottle. The concentration of nitrobenzene in the reaction system is 30 mg / L.

[0040] (3) Absorb 0.3 mL of the clay mineral stock solution in the anaerobic box and transfer it to a serum bottle, and the clay mineral concentration in the reaction system is 0.1 g / L.

[0041] (4) Take 3mL of humic acid stock solution in the anaerobic box and transfer it to the serum bottle, and the ...

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Abstract

The invention discloses a method for promoting biological reduction of nitrobenzene by synergy of clay mineral and humic acid and belongs to the technical field of biological treatment of pollutants. The method comprises the following steps of adding the clay mineral and the humic acid into a solution containing the nitrobenzene, transferring into a serum bottle, adding dissimilatory metal reduction bacterial cells, then putting the serum bottle into a constant-temperature shaking table with the rotating speed of 150rpm and the temperature of 30 DEG C to carry out light-blocking reaction for 60 hours. The clay mineral and the humic acid used in the method are NAu-2 and ESHA respectively, the serum bottle is oscillated for 24 hours before use so as to mix the NAu-2 and the ESHA uniformly and achieve balanced absorption. The method disclosed by the invention has the advantages that by synergic use of the clay mineral and the humic acid, the biological reduction of the nitrobenzene can be effectively promoted, multi-time repeated utilization can be achieved, the promotion effect is stable and is superior to that under the condition of single addition of the clay mineral and the humic acid respectively; and the clay mineral and the humic acid are natural soil components, the distribution is wide, the price is low and the obtaining is easy, so that promotion and application in practical conditions are convenient.

Description

technical field [0001] The invention belongs to the technical field of pollutant biological treatment and provides a method for synergistically promoting the reduction of nitrobenzene by microorganisms by using clay ore and humic acid. Background technique [0002] Nitrobenzene is a compound formed by replacing a hydrogen atom on the benzene ring with a nitro group. The molecular formula is C 6 h 5 NO 2 , also known as dense spot oil, bitter almond oil. It is usually used as an intermediate in the synthesis of organic matter such as dyes, explosives, rubber, and pesticides, or as a raw material for the production of aniline, and widely exists in wastewater from industries such as dyes, pesticides, medicine, and petrochemicals. Nitrobenzene is a refractory organic compound with stable chemical properties, carcinogenic and mutagenic to organisms, and causes persistent pollution of water and soil after entering the environment. Therefore, the pollution control of nitrobenze...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/28C02F101/38
CPCC02F3/28C02F2101/38
Inventor 柳广飞仇爽周集体金若菲
Owner DALIAN UNIV OF TECH
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