A method of detecting a plurality of pesticide residues in silkworm pupae

A detection method, silkworm chrysalis technology, is applied in the field of detection of various pesticide residues in silkworm chrysalis, which can solve the problems of difficult degradation of pesticides, pollution of secondary planting food chain products, etc., and achieve high recovery rate, strong applicability, and good precision.

Active Publication Date: 2017-02-08
RIZHAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] For a long time, in order to increase crop yield and income, a large amount of pesticides have been used, and some pesticides are difficult to degrade and remain in the soil in large quantities, thereby polluting secondary planting and related food chain products. , but there are no relevant standards and literature reports

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  • A method of detecting a plurality of pesticide residues in silkworm pupae
  • A method of detecting a plurality of pesticide residues in silkworm pupae
  • A method of detecting a plurality of pesticide residues in silkworm pupae

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Embodiment Construction

[0035] The present invention will be further described below in conjunction with the accompanying drawings and embodiments. It should be noted that the following description is only for explaining the present invention and not limiting its content.

[0036] Take the detection of 30 kinds of pesticide residues as an example for illustration, but the detection method of the present invention is not limited to 30 kinds of pesticide residues; the silkworm chrysalis is boiled silkworm chrysalis, but the present invention is not limited to this type of silkworm chrysalis.

[0037] 1 Experimental process

[0038] 1.1 Instruments and reagents

[0039] 1.1.1 Instruments

[0040] GC / MS / MS gas chromatography-tandem triple quadrupole mass spectrometer (Agilent7890-7000B); oscillator (IKA, Germany); Turbo Vap nitrogen drying instrument (Zymark, USA); Milli.Q ultrapure water device (USA Millipore Company); PSA / C18—SPE solid-phase extraction column (1000mg, 6mL, Aijieer).

[0041] 1.1.2 R...

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Abstract

A method of detecting a plurality of pesticide residues in silkworm pupae is disclosed. The method includes (1) a step of extracting, namely a step of extracting a silkworm pupa sample by adopting acetonitrile into which a phosphate buffer solution is added as an extracting solvent, and adopting an extracting manner that homogenization is firstly performed and oscillation is then performed to obtain an extract liquid, and performing concentration to obtain a concentrate liquid, (2) a step of purifying, namely a step of eluting the concentrate liquid for purification by adopting a mixed solution comprising acetone and n-hexane in a volume ratio of 1:1 as an eluant, collecting eluates, performing concentration and adding to a constant volume to obtain a liquid to be detected, and (3) a step of detecting contents of the plurality of pesticide residues in the liquid to be detected by adopting gas chromatography tandem triple quadrupole mass spectrometry. The method of detecting the plurality of pesticide residues in the silkworm pupae is established based on characteristics that silkworm pupae are high in oil and grease contents, relatively complex in matrix, and the like. The method is simple in operation, high in sensitivity and good in repeatability, and can be widely used for detection of the plurality of pesticide residues in the silkworm pupae.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method of various pesticide residues in silkworm chrysalis. The invention belongs to the technical field of pesticide residue extraction and detection. Background technique [0002] From ancient times to the present, my country is a big country for raising silkworms, and silkworm chrysalis is the chrysalis of the Bombyx moth of the silkworm family insect, which is the main by-product of the silk reeling industry. With the development of agricultural science and technology, the strengthening of the combination of "production, learning and research", and the continuous improvement of people's awareness of nutrition and health, the value of silkworm chrysalis has attracted widespread attention. In recent years, scientific researchers have carried out relevant research on the application of the components of silkworm chrysalis in different fields such as food, feed, chemical industr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 高尧华李林杰宋卫得刘冰刘培海王凯
Owner RIZHAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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