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Oligomer flocculating microalgae utilization method and application method thereof

A technology of oligomers and microalgae, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as refractory degradation, easy pollution, and influence of medium recycling, and achieve high separation efficiency, The effect of eliminating surface repulsion and easy operation

Active Publication Date: 2017-02-22
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] However, inorganic flocculants will affect the subsequent production of energy microalgae, while polymer flocculants are expensive to use, difficult to degrade, and prone to pollution, which limits their large-scale application
In order to avoid contamination, Benemann and Oswald proposed the use of microbial flocculants, but the cost of microbial flocculants is high, and the recycling of the medium is also affected by the residual microbial dose

Method used

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  • Oligomer flocculating microalgae utilization method and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Flocculation separation of Scenedesmus sp

[0037] (1) Preparation of oligomers

[0038] Step 1: Take 5mL of distilled water in a 50mL beaker, slowly add 15mL of concentrated sulfuric acid (analytical grade), let stand and cool to room temperature, and set aside.

[0039] Step 2: Take 291mL of absolute ethanol in a 500mL clean beaker, then add 9mL of triaminotrimethoxysilane, and stir for 1min.

[0040]Step 3: Put 1 mL of the solution in Step 1 into the solution in Step 2, stir for 1 min, let stand for 21.5 h, centrifuge, rinse with absolute ethanol three times, and then dry in a vacuum oven at 80°C for 12 h to obtain the oligomer mixture. Characterize it, the results are as follows:

[0041] 29 SiNMR (119MHz,D 2 O): δ-68.67, -58.94, -48.97;

[0042] 1 HNMR (300MHz,D 2 O,25℃):δ3.54(q,nH),δ2.91(t,6nH),δ1.69(d,6nH),δ1.07(t,1.5nH),δ0.62(t,6nH );

[0043] 13 CNMR (75MH Z, D. 2 O, 25°C): δ57.42, 41.71, 20.62, 16.79, 8.67;

[0044] FTIR (KBr, cm -1 ):...

Embodiment 2

[0052] Example 2: Observation of cell activity

[0053] Cell viability before and after flocculation of Scenedesmus sp in Example 1 was determined using Evans blue solution staining (see figure 1 ). The specific operation is as follows: take 1 mL of the microalgae liquid after flocculation of the microalgae in Example 1, remove the supernatant after centrifugation, then add 100 μL of 1 wt% Evans blue solution to the centrifuged microalgae, and incubate at room temperature for 3 hours. Then wash twice with distilled water to remove excess and released dye. Then the washed microalgae were observed and photographed with an optical microscope. Since the Evans blue solution diffuses into the protoplasm of the destroyed microalgae and stains it with color, the destroyed and dissolved microalgae will change from green to blue, while the intact cells remain the same green.

[0054] in figure 1 Middle: Microalgal cells before flocculation (a); Microalgal cells after heating at 121...

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Abstract

The invention belongs to the technical field of biomass energy, and discloses an oligomer flocculating microalgae utilization method and application method thereof. The utilization method comprises the steps of adding oligomer in a culture solution cultured with microalgae, stirring to make the microalgae flocculating and sinking, after stewing and layering, separating the upper layer clear solution, to obtain the flocculated culture solution and microalgae; the oligomer is the mixed oligomer obtained after hydrolysis of the amino silane coupling agent alcobolic solution. The method through adding oligomer in the microalgae culture solution, through the action of the protonated amino group in oligomer with the negative charge brought by hydroxyl or sulfate radical and other group on the microalgae cell surface, achieves the effect of removing the surface repulsive force among the cells, and breaks the balance state of microalgae in the culture solution, meanwhile, because of the spatial steric hindrance of olgomer, the microalgae cells are made connected in a bridging mode and react by agglomeration settlement, thus achieving the effect of flocculation harvesting, the harvesting efficiency can reach 90% or above, can be widely applied to the Isolation and harvesting of microalgae.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, in particular to a method for flocculating microalgae using oligomers and its application. Background technique [0002] Microalgae (Microalga) is a unique autotrophic one of the most primitive organisms, photosynthetic organisms that can directly convert solar energy into chemical energy. Microalgae are small in size, simple in structure, have many types, wide distribution, strong ability to adapt to the environment, short growth cycle, fast growth rate, and high oil content. [0003] Microalgae is an economical and efficient renewable biomass energy. Compared with other oil crops, microalgae does not need to occupy arable land resources, does not compete with agriculture, and will not cause a food crisis. Its biggest advantage is that it can accumulate a high content of oil, accounting for about 15-80% of the dry weight of microalgae cells. It occupies an important position in the fiel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 朱毅林利芳张渊明韩博平张成武
Owner JINAN UNIVERSITY
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