Method for flocculating microalgae by using flue gas and application thereof

A microalgae and flue gas technology, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of high cost of using organic polymer flocculants, affecting the subsequent production of microalgae energy, and huge amount of flocculants, etc. problems, to achieve the effect of reducing the cost of separation and recovery, high efficiency of flocculation and separation, and eliminating the need for stirring operations.

Inactive Publication Date: 2016-10-26
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] However, the commonly used organic polymer flocculants are expensive to use, and the metal ions in the inorganic metal salt flocculants will contaminate algae cells and affect the subsequent production of microalgae energy.
Secondly, in view of the fact that the production of microalgae bioenergy needs to deal with an extremely large amount of microalgae culture medium, the amount of flocculant will also be huge
In addition, the above solid and liquid flocculants need to be stirred evenly when putting in the microalgae culture medium, which will also cause huge energy consumption when processing large batches

Method used

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  • Method for flocculating microalgae by using flue gas and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Flocculation of Scenedesmus obliquus

[0040] Scenedesmus obliquus (Scenedesmus obliquus, purchased from the Algae Collection Center, University of Texas at Austin, USA) was cultivated in the incubator with conventional BG-11 culture medium. Take 2LBG-11 culture solution for cultivation, and adjust the microalgae implantation amount to OD 750 ≈0.6, at a temperature of 25°C, with an average light intensity of 200μmol·m -2 ·s -1 Cool white fluorescent light for 24 hours, and continuously pass through the air containing 1% CO 2 (v / v) of air. The cultivation time is 16 days as a cycle.

[0041] When the dry weight of Scenedesmus obliquus in the culture medium is 1.0-6.0g / L, the flue gas containing 1000ppm sulfur dioxide is passed into the microalgae culture medium until obvious flocs are produced, and the aeration is stopped after about 35-50 minutes (the aeration time depends on the dryness). Weight increases), naturally settled for 30 minutes, poured out t...

Embodiment 2

[0044] Example 2: Flocculation of Scenedesmus sp

[0045] Scenedesmus sp (Scenedesmus sp, purchased from the Algae Collection Center, University of Texas at Austin, USA) was cultivated in the incubator with BG-11 culture medium. Measure 2L of BG-11 culture solution for cultivation, the amount of microalgae implantation, cultivation conditions and cultivation time are the same as in Example 1.

[0046] When the dry weight of Scenedesmus sp in the culture medium is 1.0-6.0g / L, pass the flue gas containing 800ppm sulfur dioxide into the microalgae culture medium until obvious flocs are produced, and stop the aeration after about 50-70 minutes (the aeration time is based on the dry weight increase with increasing), natural sedimentation for 30min, pour out the supernatant, and Scenedesmus sp can be obtained. Calculated by the above formula, the separation rate after standing for 30 minutes was 92.3-98.2%.

Embodiment 3

[0047] Example 3: Flocculation of Chlorococcus ellipsoides

[0048] Chlorococcum ellipsoideum (Chlorococcum ellipsoideum, purchased from the Algae Collection Center, University of Texas at Austin, USA) was cultivated in the incubator with BG-11 culture medium. Measure 2L of BG-11 culture solution for cultivation, the amount of microalgae implantation, cultivation conditions and cultivation time are the same as in Example 1.

[0049] When the dry weight of Chlorococcus ellipsoides in the culture solution is 1.0-6.0g / L, the flue gas containing 600ppm sulfur dioxide is passed into the microalgae culture solution until obvious flocs are produced, and the aeration is stopped after about 80-100 minutes (the aeration time depends on dry weight increases), naturally settle for 30 minutes, pour out the supernatant, and then get Pidelodioides. Calculated by the above formula, the separation rate after standing for 30 minutes was 90.34-91.90%.

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Abstract

The invention discloses a method for flocculating microalgae by using flue gas and application thereof, belonging to the technical field of biomass energy. The method comprises the following concrete steps: introducing flue gas containing sulfur dioxide into a nutrient solution where microalgae are cultured until microalga agglomeration algae is generated; carrying out standing; and removing supernatant so as to obtain flocculated microalgae; wherein the microalgae refer to fresh-water microalgae. According to the invention, flue gas from a coal-fired plant is continuously introduced into the microalga nutrient solution and sulfur dioxide hydrate in the flue gas interacts with extracellular polysaccharide organic matters secreted by the surfaces of microalgae, so the negatively-charged extracellular polysaccharide organic matters bond with the sulfur dioxide hydrate and free hydrogen ions produced thereof, surface expulsive force among cells is eliminated, and the balance state of microalgae in the nutrient solution is broken; thus, agglomeration and settlement of microalga cells are realized, and harvesting efficiency reaches 90% or above. The method is simple to operate and high in separation efficiency, has almost no cost and can be applied to separation of microalgae.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, and in particular relates to a method for flocculating microalgae by using flue gas and its application. Background technique [0002] Algae are considered to be one of the oldest life forms, a primitive plant that can perform photosynthesis even without primitive plants with roots, stems, and leaves. The simple structure of algae cells allows them to adapt to different environmental conditions and survive for a long time, making them one of the oldest primary producers on earth. [0003] Compared with other oil crops, the biggest advantage of microalgae as a bioenergy is that it has a high fat content, accounting for 30-70% of the dry weight of microalgae cells. At the same time, based on its rapid growth ability and the characteristics of water body culture, its oil production per unit area is astonishing, and the annual oil production reaches nearly 100,000 liters / ha. [0004] Compare...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 朱毅张渊明张成武吴劲衡林利芳
Owner JINAN UNIVERSITY
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