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A process for increasing the acid production of acidogenic bacillus coagulans

A technology of Bacillus coagulans and acid content, applied in the field of microbial culture, can solve the problems of hard to buy, high price, difficult source of strains, etc., and achieve the effect of saving cost and accelerating metabolism

Active Publication Date: 2019-07-02
上海国龙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, the high-yielding Bacillus coagulans obtained by occasional screening is generally fermented to produce lactic acid. However, such strains are not easy to source, the price is high, and it is even difficult to buy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The bacterial strain used in the examples of the present invention was purchased from Shaoguan Yilinquan Biotechnology Co., Ltd., and is Bacillus coagulans for common feed.

[0027] A kind of technology that improves bacillus coagulans acid production, its steps are as follows:

[0028] (1) Preparation of seed medium:

[0029] The formula is as follows: glucose 60g / L, yeast powder 6g / L, peptone 10g / L, beef extract 3g / L, plant compound extract 3g / L, manganese sulfate 0.005g / L, NaCl 2g / L, potassium dihydrogen phosphate 3g / L L, magnesium sulfate 0.02g / L;

[0030] The preparation method of the plant compound extract is as follows:

[0031] According to parts by weight, weigh 15 parts of Tribulus terrestris, 2 parts of hawthorn, and 3 parts of seabed coconut, add 100 parts of deionized water, keep boiling for 20 minutes, then let it stand for 24 hours, and then filter with 200 mesh filter cloth to obtain the extract and dregs ;

[0032] The obtained medicinal residues an...

Embodiment 2

[0042] A kind of technology that improves bacillus coagulans acid production, its steps are as follows:

[0043] (1) Preparation of seed medium:

[0044] The formula is as follows: glucose 60g / L, yeast powder 6g / L, peptone 10g / L, beef extract 3g / L, plant compound extract 4g / L, manganese sulfate 0.005g / L, NaCl 2g / L, potassium dihydrogen phosphate 3g / L L, magnesium sulfate 0.02g / L;

[0045] The preparation method of the plant compound extract is as follows:

[0046] According to parts by weight, weigh 10 parts of Tribulus terrestris, 2 parts of hawthorn, and 3 parts of seabed coconut, add 100 parts of deionized water, keep boiling for 20 minutes, then let it stand for 24 hours, and then filter with 200-mesh filter cloth to obtain the extract and dregs ;

[0047] The obtained medicinal residues and deionized water were added into deionized water according to the weight volume ratio of 1:6, boiled again and kept boiling for 30 minutes, filtered to obtain the filtrate;

[0048]...

Embodiment 3

[0057]A kind of technology that improves bacillus coagulans acid production, its steps are as follows:

[0058] (1) Preparation of seed medium:

[0059] The formula is as follows: glucose 60g / L, yeast powder 6g / L, peptone 10g / L, beef extract 3g / L, plant compound extract 3g / L, manganese sulfate 0.005g / L, NaCl 2g / L, potassium dihydrogen phosphate 3g / L L, magnesium sulfate 0.02g / L;

[0060] The preparation method of the plant compound extract is as follows:

[0061] According to parts by weight, weigh 14 parts of Tribulus terrestris, 2 parts of hawthorn, and 3 parts of seabed coconut, add 100 parts of deionized water, keep boiling for 20 minutes, then let it stand for 24 hours, and then filter with 200-mesh filter cloth to obtain the extract and dregs ;

[0062] The obtained medicinal residues and deionized water were added into deionized water according to the weight volume ratio of 1:6, boiled again and kept boiling for 30 minutes, filtered to obtain the filtrate;

[0063] ...

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PUM

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Abstract

The invention discloses a technology for improving acid production of acid-producing bacillus coagulans. The technology comprises the steps of 1, preparation of a seed medium, wherein the seed medium is prepared from, 60 g / L of glucose, 6 g / L of yeast powder, 10 g / L of peptone, 3 g / L of beef extract, 3-4 g / L of plant compound extracting solution, 0.05 g / L of manganese sulfate, 2 g / L of NaCl, 3 g / L of monopotassium phosphate and 0.02 g / L of magnesium sulfate; 2, seed culture, wherein the temperature is 40 DEG C, the initial pH value is 7.0, the rotate speed is 250 r / min, the loading volume is 40 mL (a 250 mL flask), the inoculation amount is 6% (v / v), and the fermentation time is 5-8 hours; 3, preparation of a fermentation medium; 4, fermental cultivation, wherein the inoculation amount is 5% (v / v), the temperature is 20 DEG C, low-temperature culture is conducted for 200-230 min under sufficient oxygen supply, then the temperature is increased to 48 DEG C for anaerobic fermentation for 60 h; the glucose is supplemented twice during the period of fermentation, and the final concentration is controlled to be less than or equal to 20% after the glucose is supplemented.

Description

technical field [0001] The invention relates to a process for increasing the acid production of acidogenic bacillus coagulans, belonging to the field of microorganism cultivation. Background technique [0002] Lactic acid is currently recognized as one of the three major organic acids in the world, and it is widely used in many fields such as food, medicine, chemical industry, tanning, textile, environmental protection and agriculture. [0003] Bacillus coagulans was first isolated and described in detail in 1932 because of its large-scale coagulation of condensed milk and the rancidity of tomato, potato and other products. So far, many studies have shown that Bacillus coagulans, as a unique new spore probiotic, not only has the lactic acid-producing characteristics of lactic acid bacteria, but also has the rich enzyme system of Bacillus, strong stress resistance, high temperature and high pressure resistance, and stable storage. characteristic. As a rising star of the pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C12R1/07
CPCC12P7/56
Inventor 姜蒙钟诚雷雨蒋正芳高儒松
Owner 上海国龙生物科技有限公司