Method for detecting esophagus cancer susceptibility gene PTEN polymorphism with Hae III

A technology for susceptibility genes and esophageal cancer, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve problems such as small scope of application, high detection cost, and complicated operation, and the method is simple and easy Good, easy to recognize, low cost effect
CN106434877AInactive Publication Date: 2017-02-22ZHENGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHENGZHOU UNIV
Publication Date
2017-02-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting esophagus cancer susceptibility gene PTEN polymorphism with Hae III. Primer sequences are shown as SEQ ID NO:1 and SEQ ID NO:2. The method includes the steps that genomic DNA of a sample is extracted; a forward primer and a reverse primer for amplifying sequences near the human PTEN gene polymorphism rs701848 site are provided, PCR amplification is conducted with the extracted genomic DNA to be detected as the template, and an amplification product is obtained; the obtained amplification product is subjected to digestion with the restriction enzyme Hae III, and a corresponding digestion product is obtained; the digestion product is subjected to electrophoresis with 3% agarose gel so as to judge the genotypes of PTEN gene polymorphism rs701848. The method is simple, easy to implement, rapid, efficient and low in cost, the digestion result is easy to identify, and a simple way is provided for genotyping of esophagus cancer susceptibility gene PTEN polymorphism rs701848.
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Description

technical field

[0001] The invention belongs to the technical field of gene polymorphism detection, in particular to a HaeIII method for detecting the polymorphism of esophageal cancer susceptibility gene PTEN. Background technique

[0002] At present, single nucleotide polymorphism (single nucleotide polymorphism, SNP) is the result of environmental selection in the long-term evolution of human beings, and has become the marker of the third generation of molecular inheritance, with the advantages of high marker density, stability, and easy typing and detection , is one of the genetic basis of differences between races and individuals, has been identified as the basis of susceptibility to a variety of genetically related diseases, and has become an evaluation index for the degree of disease risk. PCR-RFLP (Polymerase Chain Reaction restriction fragment length polymorphisms) is an in vitro enzymatic synthesis of specific nucleotide fragment technology that simulates the in vi...

Claims

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