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Method for testing protein in tobacco and tobacco products through automatic azotometer

A nitrogen analyzer and protein technology, applied in nitrogen determination, chemical method analysis, instruments, etc., can solve the problems of long detection cycle, high labor intensity, safety risks for human body and environment, etc., to achieve high accuracy and reduce work. Labor intensity, the effect of reducing safety risks and environmental pollution

Inactive Publication Date: 2017-02-22
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] In view of the problems of high labor intensity, long detection period, and safety risks to the human body and the environment in the current method for the determination of protein in tobacco and products, by changing the catalyst type when the sample is digested, the Kjeldahl nitrogen method is used, and the automatic determination method is used. Nitrogen meter to achieve accurate, efficient and safe determination of protein content in tobacco and its products

Method used

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  • Method for testing protein in tobacco and tobacco products through automatic azotometer
  • Method for testing protein in tobacco and tobacco products through automatic azotometer

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Embodiment 1

[0019] ① Accurately weigh 0.5000g of the flue-cured tobacco sample that passed through the 0.45mm sieve into a 300mL conical flask, weigh 15 parts in total, sample number 1-15, add 10.0mg casein standard sample to No. Add 20.0 mg casein standard sample to No. 10, 11, and 12, add 30.0 mg casein standard sample to No. 13, 14, and 15, and do 3 blank tests at the same time;

[0020] ② Add 75mL of 0.5% acetic acid solution to each Erlenmeyer flask, heat and keep boiling for 15min;

[0021] ③Quickly use quantitative filter paper with a diameter of 150mm to perform suction filtration in a vacuum filtration device, and rinse the Erlenmeyer flask and the precipitate with 0.5% acetic acid solution until the filtrate is colorless, then transfer the filter paper and the precipitate to a digestion tube with a volume of 250mL;

[0022] ④Add 1.5g K to the digestive tube 2 SO 4 and 0.15g CuSO 4 • 5H 2 0, then add 15mL of concentrated sulfuric acid;

[0023] ⑤ Place the digestive tube on ...

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Abstract

The invention discloses a method for testing protein in tobacco and tobacco products through an automatic azotometer. Samples of tobacco and tobacco products containing about 30-60 mg of protein are weighed accurately and put into a conical flask with the capacity of 300 mL, and 75 mL of a 0.5% acetic acid solution is added and heated and kept boiling for 15 min. Suction filtration is conducted quickly in a vacuum suction filtration device through quantitative filter paper, the conical flask and precipitates are washed with a 0.5% acetic acid solution until filtrate is colorless, and then the filter paper and the precipitates are transferred to a digestive tube. 1.5 g of K2SO4 and 0.15 g of CuSO4*5H20 are added to the digestive tube, 15 mL of concentrated sulfuric acid is added, and the digestive tube is put on a digestive apparatus, heated to 420 DEG C and kept at the temperature for 1 h until digestion is finished. After being cooled to the room temperature, the digestive tube is put on the automatic azotometer for testing of the content of protein.

Description

technical field [0001] The invention relates to the field of tobacco and product detection, in particular to a method for measuring protein in tobacco and product by an automatic azotometer. Background technique [0002] Protein is a kind of nitrogen-containing biopolymer compound existing in cells, which plays a decisive role in life phenomena. Nitrogen in tobacco leaves mainly exists in the form of protein and is rich in content. The protein in tobacco leaves is the precursor of quinoline, HCN and several other harmful substances when it is burned, and it has the smell of burning feathers, which will increase the bitterness of the smoke and have adverse effects on human health and the quality of tobacco leaves. Therefore, Accurate and efficient detection of protein content in tobacco and its products is an important task in tobacco quality inspection. [0003] There are many methods for the determination of protein. Affected by factors such as tobacco quality and safety e...

Claims

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Application Information

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IPC IPC(8): G01N31/00
CPCG01N31/002
Inventor 郭先锋邱军林樱楠鲁世军孔鹏王允白孔凡玉宁扬艾红丽
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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