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Bird's nest polypeptide and preparation method thereof

A bird's nest polypeptide and bird's nest technology, applied in the field of bird's nest polypeptide and its preparation, can solve the problems of unsatisfactory absorption and utilization, low absorption efficiency of bird's nest, and no mention of the proportion of sialic acid content in the extract, and has a good prospect for industrial application , Low production cost, and the effect of improving sub-health symptoms

Active Publication Date: 2017-03-08
SICHUAN KELUN XINGUANG HEALTH PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the nutrition and cosmetic sample industry, the traditional processing of bird’s nest is mainly the rough processing method of steaming. The bird’s nest obtained by this processing method basically maintains the original macromolecular protein structure in the sample preparation. Because the molecular weight of the protein is too large, it cannot be absorbed by the human body. Complete absorption makes the absorption efficiency of bird's nest lower, so it is very necessary to improve the absorption and utilization of protein in bird's nest through appropriate protein modification methods
[0005] Currently disclosed data on protein modification of bird’s nest, such as the patent publication No. CN1858225A, discloses a method for degrading bird’s nest with ficin, and obtains bird’s nest polypeptides with a molecular weight distribution of 200-1000D, but does not mention that in the extract The content ratio of sialic acid; the patent of publication number CN104498572A discloses a bird’s nest compound enzymatic hydrolysis processing technology. The enzymatic hydrolysis is realized by adding animal protease, neutral protease and flavor enzyme at the same time. The content is 10.5%, but the number average molecular weight of the product is 5.83*10 3 , the weight average molecular weight is 1.89*10 4 , the absorption and utilization of this product in the human body are still not ideal

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  • Bird's nest polypeptide and preparation method thereof
  • Bird's nest polypeptide and preparation method thereof
  • Bird's nest polypeptide and preparation method thereof

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Effect test

preparation example Construction

[0053] Preparation of control standard sample solution: Accurately weigh 2 mg of sialic acid (N-acetylneuraminic acid) standard sample, and treat it in the same way as the test sample to prepare a control sample solution of about 0.2 mg / ml.

[0054] Verification: 20 microliters of the above-mentioned control sample and enzymatic hydrolyzate sample solution were drawn respectively, and injected into a high-performance liquid chromatograph for verification.

[0055] (5) Calculation method:

[0056] Calculation formula:

[0057]

[0058] In the formula:

[0059] S 供 : main peak peak area in the chromatogram of need testing solution; S 对 : the area of ​​the main peak in the reference substance solution chromatogram;

[0060] m 对 : the sample weight (mg) of the reference substance; P 对 : the percentage content of the reference substance; m 供 : the sample weight of the test product (mg);

[0061] V 对 : the dilution factor of the reference substance; V 供 : The dilution fac...

Embodiment 1

[0094] The preparation of embodiment 1 bird's nest polypeptide of the present invention

[0095] 1. Raw materials: imported Malaysian bird's nest.

[0096] 2. Preparation method:

[0097] Wash 100 grams of bird’s nest crude sample with water, add 1000 ml of deionized water and heat up to 85°C for 30 minutes, then cool down to 45°C, adjust the pH value to 8, add 3 grams of alcalase enzyme, degrade for 2 hours, and then re- Add 4 grams of A1398 enzyme, react for 2 hours, the bird’s nest dissolves into a white translucent reaction solution, then adjust the pH value to 6, add lysozyme, react at 40°C for 2 hours, then raise the temperature to 80°C, and keep it warm for 15 minutes to inactivate the enzyme , filtered while hot. The supernatant is distilled under reduced pressure at a vacuum degree of 0.06-0.10Mpa and a temperature of 40-65°C, and finally freeze-dried to obtain the bird's nest polypeptide of the present invention.

[0098] 3. Test results:

[0099] (1) Weight aver...

Embodiment 2

[0104] Embodiment 2 The preparation of bird's nest polypeptide of the present invention

[0105] 1. Raw materials: imported Malaysian bird's nest.

[0106] 2. Preparation method:

[0107] Wash 100 grams of bird’s nest crude sample with water, add 1200 ml of deionized water and raise the temperature to 80°C for 60 minutes, then cool down to 65°C, adjust the pH value to 9, add 8 grams of trypsin, degrade for 12 hours, and then re- Add 4 grams of Neutrase enzyme, react for 12 hours, the bird's nest dissolves into a white translucent reaction solution, add lysozyme, react at 40°C for 12 hours, then raise the temperature to 90°C, keep warm for 30 minutes to inactivate the enzyme, and filter while it is hot. The supernatant is distilled under reduced pressure at a vacuum degree of 0.06-0.10Mpa and a temperature of 40-65°C, and finally freeze-dried to obtain the bird's nest polypeptide of the present invention.

[0108] 3. Test results:

[0109] (1) Weight average molecular weight...

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Abstract

The invention provides a bird's nest polypeptide. The weight-average molecular weight of the bird's nest polypeptide is smaller than 2000Da and the weight percent content of sialic acid is more than 7%. The invention further provides a preparation method and application of the bird's nest polypeptide. The bird's nest polypeptide provided by the invention is easy to digest and absorb in a human body and can be used for rapidly maintaining beauty and protecting skins and improving sub-health symptoms; and the bird's nest polypeptide is simple and convenient in preparation process, low in production cost and good in industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of bird's nest processing, and in particular relates to a bird's nest polypeptide and a preparation method thereof. Background technique [0002] Bird's nest, also counting swallow vegetables, swallow roots, and swallow vegetables, refers to the nests built by mixing saliva and down feathers with some swifts of the swift family Swift family and several swiftlets of the genus Swiftlet. Bird's nest is flat in nature, sweet in taste, nourishes the lungs and nourishes yin, has the effects of nourishing yin and moistening dryness, nourishing qi and invigorating the middle, and can relieve symptoms such as cough and hemoptysis. It has been known as a nourishing treasure since the Tang Dynasty. Ginseng and other traditional Chinese medicine replenishment samples. [0003] Bird's nest is rich in nutrients, including protein, polysaccharides, lipids, trace elements and sialic acid. Among them, protein is the main c...

Claims

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Application Information

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IPC IPC(8): A23J3/34A23J1/10A23L33/18A61K38/01A61P17/00A61P17/02A61P37/04
CPCA23J1/10A23J3/348A61K38/012
Inventor 史宗洁张涛朱筱莉刘强杨建光
Owner SICHUAN KELUN XINGUANG HEALTH PHARMA