Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Recombinant bacillus calmette-guerin vaccine strain with over-expression mycobacterium tuberculosis Rv3586 and application of recombinant bacillus calmette-guerin vaccine strain

A technology of Mycobacterium tuberculosis and recombinant BCG, applied in the field of tuberculosis vaccine

Active Publication Date: 2017-03-08
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, rBCG that can replace traditional BCG has not yet been obtained, indicating that it is still necessary to make full use of existing research results, extensively screen new mechanism of action antigens, or improve vaccine construction methods or immunization strategies, and more effective rBCG and its immunization methods have been obtained

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recombinant bacillus calmette-guerin vaccine strain with over-expression mycobacterium tuberculosis Rv3586 and application of recombinant bacillus calmette-guerin vaccine strain
  • Recombinant bacillus calmette-guerin vaccine strain with over-expression mycobacterium tuberculosis Rv3586 and application of recombinant bacillus calmette-guerin vaccine strain
  • Recombinant bacillus calmette-guerin vaccine strain with over-expression mycobacterium tuberculosis Rv3586 and application of recombinant bacillus calmette-guerin vaccine strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0044] Construction of Rv3586 Escherichia coli-Mycobacterium Shuttle Expression Vector

[0045] Primers were designed according to the genome Rv3586 gene sequence of the Mtb H37Rv strain:

[0046] P1: 5'-tttaagcttatgcacgctgtgactcgtccgacc-3' HindIII site

[0047] P2: 5'-gcgaagcttaaggcggataattattgatcgc-3' HindIII site

[0048] Primers were synthesized by Shanghai Sangon Biotechnology Co., Ltd. Use the genomic DNA of Mtb H37Rv strain as a template to amplify the target gene by PCR. Reaction parameters: pre-denaturation at 95°C for 5 minutes, denaturation at 95°C for 30 seconds, renaturation at 57°C for 1 minute, extension at 72°C for 100 seconds, 35 cycles, and extension at 72°C for 5 minutes after the last cycle . 1% agarose gel electrophoresis analysis showed that a 1 100bp target band was amplified ( figure 1 ). The target fragment was recovered by the gel recovery kit, digested with HindIII, and the digested product was recovered by 1% agarose gel electrophoresis. T4 DN...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a recombinant bacillus calmette-guerin vaccine strain with over-expression mycobacterium tuberculosis Rv3586 and application of the recombinant bacillus calmette-guerin vaccine strain. Plasmids with encoding genes of the mycobacterium tuberculosis Rv3586 are transformed into bacillus calmette-guerin vaccine, and the recombinant bacillus calmette-guerin vaccine strain can be obtained by means of screening, is called as Bacillus calmette-Guerin rBCG-DisA and is called as rBCG-DisA for short, and a preservation number of the bacillus calmette-guerin vaccine strain is CCTCC M 2016335. The recombinant bacillus calmette-guerin vaccine strain and the application have the advantages that the recombinant bacillus calmette-guerin vaccine strain with the over-expression mycobacterium tuberculosis Rv3586 has merits of target antigens and bacillus calmette-guerin vaccine and can be used for individual immunization, or booster immunization can be carried out by the recombinant bacillus calmette-guerin vaccine strain and mycobacterium tuberculosis Ag85B-ESAT6 subunit vaccine, high immune response of normal and affected mice can be induced, the mycobacterium-resistant protective ability of organisms can be improved, and accordingly the recombinant bacillus calmette-guerin vaccine strain has an excellent application prospect.

Description

technical field [0001] The invention belongs to the field of tuberculosis vaccines. The invention relates to a recombinant BCG strain overexpressing Mycobacterium tuberculosis Rv3586. The present invention also relates to the application of the recombinant BCG strain alone or in combination with the subunit vaccine Ag85B-ESAT6 (AE) in tuberculosis preventive and therapeutic vaccines and preparations. Background technique [0002] Tuberculosis Epidemic Status [0003] Tuberculosis (TB) is still the most serious infectious disease worldwide. Mycobacterium tuberculosis (Mtb) is the pathogen of TB, and about 1 / 3 of the world's population has been infected with Mtb. According to the 2014 WHO report, there were approximately 9.6 million new TB cases worldwide. my country is one of the 22 countries with a high TB ​​burden in the world. In 2014, the number of new TB patients in my country was 930,000, ranking third in the world after India and Indonesia. The incidence of TB is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/21C12N15/74C12N15/60A61K39/04A61P31/06C12R1/32
CPCA61K39/04A61K2039/523C12N9/88C12Y406/01001C12N1/205C12R2001/32
Inventor 柏银兰徐志凯王立飞康健路延之宁唤唤吴俊杰宋世杰姚庆港
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com