A method for cultivating improved disease-resistant fish species based on genome-wide selection
A whole-genome and whole-genome sequencing technology, which is applied in the field of breeding disease-resistant varieties of fish, can solve problems such as the lack of whole-genome selection methods, and achieve the improvement of disease resistance and breeding survival rate, efficient cultivation, wide application value and promotion prospects. Effect
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[0035] Taking flounder and half-smooth tongue sole as examples, the breeding method of fish disease-resistant improved species based on whole-genome selection will be described in detail in conjunction with the accompanying drawings:
[0036] 1. Establishment of fish disease-resistant reference populations and determination of phenotypic traits
[0037] When the established fish family fries grow to a total length of 8-15 cm, pathogenic microorganisms are used to artificially infect the fish family fry with pathogenic bacteria, and the dead fry of each family are collected at different times after the onset of pathogenic bacteria infection, and the death time and time of the fish fry are recorded. Phenotypic data such as full length, weight, and body width, while collecting surviving individuals after infection and recording the data of full length, weight, and body width, and using the collected data of surviving individuals and dead individuals as phenotypic parameters for ev...
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