Method for covering culture medium when test-tube plantlet is transported
A technology of culture medium and test-tube seedlings, which is applied in application, horticultural methods, botany equipment and methods, etc., can solve the problems of easily damaged culture medium, contamination and damage of test-tube seedlings, etc., and achieve low cost, high selectivity, and simple process Effect
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[0010] Specific embodiment 1: This embodiment is a method for covering the culture medium when transporting test tube plantlets, which is specifically carried out according to the following steps:
[0011] Put MS medium, sucrose and pyrophosphate into distilled water and mix, heat to completely dissolve, adjust to pH 6.0-8.0 with 10mol / L NaOH solution to obtain a mixed solution; put agar into the mixed solution, then Put it into the sterilization pot and sterilize it at 121℃ for 20 minutes. After taking it out, let it cool to 45℃ at room temperature to obtain a liquid coagulant, and then pour the liquid coagulant into a glass funnel on the ultra-clean workbench. The temperature is 8℃ and the test tube seedlings and solid medium are contained in the Erlenmeyer flask until the liquid coagulant covers the top of the solid medium and the thickness is 1cm. When injecting the liquid coagulant, it must be placed against the inner wall of the Erlenmeyer flask. Contact with the stems and ...
Example Embodiment
[0013] Specific embodiment two: this embodiment is different from specific embodiment one in that the pyrophosphate is sodium pyrophosphate or sodium acid pyrophosphate. Others are the same as the specific implementation.
Example Embodiment
[0014] Specific embodiment three: this embodiment is different from specific embodiment one in that the temperature is 8° C. and the Erlenmeyer flask containing the test tube plantlets is placed in the refrigerator. Others are the same as the first embodiment.
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