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Method for covering culture medium when test-tube plantlet is transported

A technology of culture medium and test-tube seedlings, which is applied in application, horticultural methods, botany equipment and methods, etc., can solve the problems of easily damaged culture medium, contamination and damage of test-tube seedlings, etc., and achieve low cost, high selectivity, and simple process Effect

Active Publication Date: 2017-03-15
黑龙江省农业科学院植物脱毒苗木研究所
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] The present invention aims to solve the technical problem that the medium of the existing test-tube plantlets is easily damaged during transportation, and the test-tube plantlets are damaged by pollution, and provides a method for covering the medium when transporting test-tube plantlets

Method used

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  • Method for covering culture medium when test-tube plantlet is transported

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specific Embodiment approach 1

[0010] Specific embodiment one: this embodiment is a method for covering the culture medium when it is applied to the transportation of test-tube plantlets, specifically according to the following steps:

[0011] Mix MS medium, sucrose and pyrophosphate in distilled water, heat until completely dissolved, and adjust the pH value to 6.0-8.0 with 10mol / L NaOH solution to obtain a mixed solution; put agar into the mixed solution, and then Put it in a sterilizer and sterilize it at 121°C for 20 minutes, take it out and let it cool down to 45°C naturally at room temperature to obtain a liquid coagulant, and then inject the liquid coagulant with a glass funnel on the ultra-clean workbench The temperature is 8°C, and the test tube seedlings and solid medium are placed in the conical flask until the liquid coagulant covers the top of the solid medium and the thickness is 1cm. When injecting the liquid coagulant, it should be injected against the inner wall of the conical flask to avoid...

specific Embodiment approach 2

[0013] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the pyrophosphate is sodium pyrophosphate or sodium acid pyrophosphate. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0014] Embodiment 3: This embodiment is different from Embodiment 1 in that: the temperature is 8° C. and the triangular flask containing the test-tube seedlings is placed in the refrigerator. Others are the same as in the first embodiment.

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Abstract

The invention relates to a method for covering a culture medium when a test-tube plantlet is transported. The method aims at solving the technical problems that a culture medium of an existing test-tube plantlet is prone to damage in the transporting process, and the test-tube plantlet is contaminated and damaged. The method includes the steps that an MS culture medium, saccharose and pyrophosphate are put in distilled water, the materials are heated till the materials are completely dissolved, the pH value is adjusted to range from 6.0 to 8.0, agar is added, the materials are put in a sterilization pot to be sterilized, the materials are naturally cooled to 45 DEG C, the materials are injected into a triangular flask filled with the test-tube plantlet, the materials are solidified at room temperature, and then the test-tube plantlet can be transported. A solidifying agent has the advantages of being free of toxins, low in residue, free of contamination, free of agent resistance, high in selectivity and wide in material source, and has no adverse reaction with an original culture medium of the test-tube plantlet. The process is simple, cost is low, compared with other solidifying agents, the concentration and pH value of the agar are increased while the ingredients of the culture medium are not changed, and loss of water and nutritional ingredients of the original culture medium is not caused.

Description

technical field [0001] The invention relates to a method for covering medium when used in transporting test-tube plantlets. Background technique [0002] The method of plant tissue culture can be used for rapid propagation of plants, and this rapid propagation technology has been widely used in mass production of various (class) plants. The medium of test-tube plantlets is easily damaged during transportation, and the test-tube plantlets are damaged by pollution. The reason is that the concentration of the general culture medium agar that plays a fixed role is between 10g / L and 25g / L, which can meet the solidification requirements within this range and has little effect on the growth of microorganisms, but its high agar concentration will cause the culture medium to Relatively hard, not easy to catch seedlings. Contents of the invention [0003] The invention aims to solve the technical problem that the medium of the existing test-tube plantlets is easily damaged during ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 申宇白艳菊范国权高艳玲张威张抒邱彩玲吕典秋陈俊莹
Owner 黑龙江省农业科学院植物脱毒苗木研究所
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