Preparation method of Nitraria tangutorum Bobr. antioxidant active extract, and products and applications thereof

A technology of antioxidant activity and extraction method, which is applied in the field of preparation of Nitraria antioxidative activity extract, and can solve the problems of unsatisfactory antioxidant activity of the extract and low yield of the extract, etc.

Active Publication Date: 2017-03-15
青海博智生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The preparation method of the antioxidant Nitraria extract in the prior art adopts the method of combining high-pressure homogenization technology, membrane separation technology and macroporous resin s

Method used

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  • Preparation method of Nitraria tangutorum Bobr. antioxidant active extract, and products and applications thereof

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preparation example Construction

[0021] like figure 1 Shown, the present invention provides a kind of preparation method of Nitraria antioxidative activity extract, comprises the steps:

[0022] S1: Mix Nitraria dried fruit with ethanol aqueous solution with a volume fraction of 30% to 95% at a solid-liquid ratio of 1g:(5 to 30)mL, cold soak for 1h to 4h, and mix the Nitraria dried fruit with the ethanol aqueous solution The mixture is extracted, and the extraction method of the extraction is one or more of heating reflux, ultrasonic extraction, microwave extraction and flash extraction, and the extraction times of the flash extraction are 3 to 5 times, and the flash extraction Each extraction time of extraction is 1min~2min, the ultrasonic temperature of the ultrasonic extraction is 40°C, the ultrasonic time is 60min, and the range of solid-liquid ratio is preferably 1g:(5~30)mL, which is considering that the ratio is greater than 1g / mL is not conducive to the extraction of the extract. If the ratio is les...

Embodiment 1

[0029] This embodiment provides a preparation method of Nitraria antioxidative activity extract, comprising the following steps:

[0030] S1: 15g of Nitraria siberia dried fruit (produced in Qinghai, batch number QH2014) was mixed with 450mL of 30% ethanol water solution by volume fraction, cold soaked for 4 hours, and then the mixture of Nitraria thorn dried fruit and ethanol water solution was ultrasonically extracted, and the ultrasonic temperature was 40℃, ultrasonic time is 60min;

[0031] S2: filtering the extracted solution, concentrating the filtered filtrate to obtain an extract;

[0032] S3: Dissolve the concentrated product in water, and use the chromatographic column filled with AB-8 macroporous resin to absorb and separate the obtained solution. The mass ratio of the product to the macroporous resin is 1:100, and the time for adsorption and separation is 12 hours;

[0033] S4: elute the chromatographic column with water until the effluent is colorless, then elute...

Embodiment 2

[0036] This embodiment provides a preparation method of Nitraria antioxidative activity extract, comprising the following steps:

[0037] S1: 15g of Nitraria siberia dried fruit (produced in Qinghai, batch number QH2014) was mixed with 450mL of 50% ethanol water solution by volume fraction, cold soaked for 4 hours, and then the mixture of Nitraria thorn dried fruit and ethanol water solution was ultrasonically extracted, and the ultrasonic temperature was 40℃, ultrasonic time is 60min;

[0038] S2: filtering the extracted solution, concentrating the filtered filtrate to obtain an extract;

[0039] S3: Dissolve the concentrated product in water, and use the chromatographic column filled with AB-8 macroporous resin to absorb and separate the obtained solution. The mass ratio of the product to the macroporous resin is 1:100, and the time for adsorption and separation is 12 hours;

[0040] S4: elute the chromatographic column with water until the effluent is colorless, then elute w...

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Abstract

The invention relates to a preparation method of a Nitraria tangutorum Bobr. antioxidant active extract, and products and applications thereof. The preparation method comprises following steps: Nitraria tangutorum Bobr. dried fruits and an ethanol aqueous solution with a volume concentration ranging from 30 to 95% are mixed at a solid-liquid ratio of 1g:(5-30)ml for 1 to 4h of cold leaching so as to extract the mixture of Nitraria tangutorum Bobr. dried fruits and the ethanol aqueous solution; an obtained extracted solution is filtered, and an obtained filtrate is concentrated; an obtained concentration product is dissolved in water, and an obtained solution is subjected to adsorption separation using a chromatographic column loaded with AB-8 macroporous resin, wherein the mass ratio of the obtained concentration product to AB-8 macroporous resin is controlled to be 1:60-1:100; the chromatographic column is subjected to elution with water until an obtained eluent becomes colourless, an ethanol aqueous solution with a volume concentration of 10 to 80% is used for elution, and ethanol eluent is collected, wherein the volume of the ethanol aqueous solution is 10 to 15 times of the column volume; the ethanol eluent is subjected to concentration drying so as to obtain the Nitraria tangutorum Bobr. antioxidant active extract. The preparation method of the Nitraria tangutorum Bobr. antioxidant active extract is capable of increasing extract yield, and increasing the antioxidant activity of the Nitraria tangutorum Bobr. antioxidant active extract.

Description

technical field [0001] The invention relates to the technical field of antioxidant active extracts, in particular to a preparation method of Nitraria antioxidative active extracts and its products and applications. Background technique [0002] Nitraria is a general designation for various small deciduous shrubs of the genus Nitraria in the family Zygophyllaceae. The main species are N. sibirica Pall. and N. tangutorum Bobr., etc. In my country, it mainly grows in the desert or semi-desert areas of Inner Mongolia, Qinghai, Xinjiang, Gansu and other places. It not only has the effect of windbreak and sand fixation, but also has high medicinal and edible value. Strengthen the spleen and stomach, nourish and strengthen, regulate menstruation and blood circulation, and promote lactation. It is often used to treat weak spleen and stomach, indigestion, neurasthenia, high blood pressure, dizziness, and colds. Modern studies have found that Nitraria thorn is rich in flavonoids and an...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P39/06
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/55
Inventor 季崇敏卢燕余小磊赵萱
Owner 青海博智生物科技有限公司
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