Preparing method and application of electrogenerated chemiluminescence sensor based on polydopamine nanometer microspheres

A technology of nano-microspheres and polydopamine, which is applied in the fields of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis through chemical reactions of materials, etc. problems, to achieve good biodegradability, large surface area, and fast response

Inactive Publication Date: 2017-03-15
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods used to detect prostate specific antigen are mainly radioimmunoassay, enzyme-linked immunoassay and fluorescent immunoassay, etc., but these methods have the disadvantages of not being able to detect instantly and quickly, having radioactive contamination, and complicated operation. The luminescence method can overcome these disadvantages well. It is an immunosensor based on the specific recognition of antigens and antibodies. Its preparation process is simple, high sensitivity, low detection limit, and fast response speed. detection

Method used

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Examples

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Embodiment 1

[0023] Example 1 A preparation method of an electrochemiluminescent sensor based on polydopamine nanospheres

[0024] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0025] (2) Drop-coat 6 μL, 0.3 mg / mL aminated multi-walled carbon nanotube / Nafion solution onto the electrode surface, immerse the electrode in 1 mM ruthenium terpyridyl solution for 30 min, and rinse the electrode surface with ultrapure water;

[0026] (3) Add 6 µL, 5 µg / mL antibody solution dropwise, dry it in a refrigerator at 4°C, and rinse the electrode surface with ultrapure water;

[0027] (4) Add 3 µL bovine serum albumin solution with a mass fraction of 1% dropwise, dry it in a refrigerator at 4°C, and rinse the electrode surface with ultrapure water;

[0028] (5) Add 6 µL, 0.0001-20 ng / mL of a series of different concentrations of prostate-specific antigen solutions, dry in a refrig...

Embodiment 2

[0030] Example 2 A preparation method of an electrochemiluminescent sensor based on polydopamine nanospheres

[0031] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0032] (2) Drop-coat 6 μL, 0.5 mg / mL aminated multi-walled carbon nanotube / Nafion solution onto the electrode surface, immerse the electrode in 1 mM ruthenium terpyridyl solution for 30 min, and rinse the electrode surface with ultrapure water;

[0033] (3) Add 6 µL, 7 µg / mL antibody solution dropwise, dry it in a refrigerator at 4°C, and rinse the electrode surface with ultrapure water;

[0034] (4) Add 3 µL bovine serum albumin solution with a mass fraction of 1% dropwise, dry it in a refrigerator at 4°C, and rinse the electrode surface with ultrapure water;

[0035] (5) Add 6 μL, 0.0001-20 ng / mL of a series of different concentrations of prostate-specific antigen solutions, dry in a refrig...

Embodiment 3

[0037] Example 3 A preparation method of an electrochemiluminescent sensor based on polydopamine nanospheres

[0038] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0039] (2) Drop-coat 6 μL, 0.7 mg / mL aminated multi-walled carbon nanotube / Nafion solution onto the electrode surface, immerse the electrode in 1 mM ruthenium terpyridyl solution for 30 min, and rinse the electrode surface with ultrapure water;

[0040] (3) Add 6 µL, 10 µg / mL antibody solution dropwise, dry it in a refrigerator at 4°C, and rinse the electrode surface with ultrapure water;

[0041] (4) Add 3 µL bovine serum albumin solution with a mass fraction of 1% dropwise, dry it in a refrigerator at 4°C, and rinse the electrode surface with ultrapure water;

[0042] (5) Add 6 μL, 0.0001-20 ng / mL of a series of different concentrations of prostate-specific antigen solutions, dry in a refri...

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Abstract

The invention relates to a preparing method of an electrogenerated chemiluminescence sensor based on polydopamine nanometer microspheres. The preparing method includes the specific steps that an amination multiwalled carbon nanotube is doped in a Nafion film for fixing terpyridine ruthenium, and the terpyridine ruthenium serves as a luminescent material. The bionic polydopamine nanometer microspheres prepared with the simple method can quench electrogenerated chemiluminescence signals of the terpyridine ruthenium; in addition, the polydopamine nanometer microspheres also have rich amino-functional groups, and can be connected with an antibody to serve as a second antibody marker, and detection of a prostate-specific antigen is achieved through the formed sandwich-type electrogenerated chemiluminescence sensor.

Description

technical field [0001] The invention relates to a preparation method of an electrochemiluminescence sensor based on polydopamine nano-microspheres, in particular to fixing ruthenium terpyridine on an electrode modified by an aminated multi-walled carbon nanotube / Nafion composite film, using a simple method The prepared polydopamine nanometer microsphere is used as a secondary antibody marker, and has a good quenching effect on ruthenium terpyridine, so as to achieve the purpose of detecting prostate specific antigen, and belongs to the technical field of new functional materials and biosensing. Background technique [0002] Prostate cancer is one of the common malignant tumors in men, and its morbidity and mortality rank the second and sixth of all male malignant tumors in the world, respectively. Compared with developed countries and regions in Europe and the United States, the morbidity and mortality of prostate cancer in China are relatively low. For these reasons, the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/48
CPCG01N21/76G01N27/48
Inventor 刘媛媛马洪敏魏琴张勇吴丹范大伟胡丽华庞雪辉
Owner UNIV OF JINAN
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