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Preparation method of electrochemical histamine sensor based on nanoporous membrane-magnetic nanoparticles

A magnetic nanoparticle and sensor technology, applied in scientific instruments, instruments, material analysis by electromagnetic means, etc., can solve the problem of insignificant blocking effect, and achieve the effects of easy measurement, high detection sensitivity, and low detection limit.

Active Publication Date: 2018-09-21
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the small size of histamine molecules, the blocking effect in the pores is not obvious

Method used

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  • Preparation method of electrochemical histamine sensor based on nanoporous membrane-magnetic nanoparticles
  • Preparation method of electrochemical histamine sensor based on nanoporous membrane-magnetic nanoparticles
  • Preparation method of electrochemical histamine sensor based on nanoporous membrane-magnetic nanoparticles

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Embodiment 1

[0028] 1. Preparation of the electrochemical impedance sensor cavity. The cavity is mainly made of PDMS material. Component A of Dow Corning DC184 encapsulant (the main component is double-bond-terminated polymethylsiloxane plus platinum catalyst) and component B (the main component is double-bond-terminated polymethylsiloxane and multifunctional Si-H-containing polymethylsiloxane) was completely mixed at a weight ratio of 10:1, poured into a mold and cured at 120°C for 2 hours to make a PDMS container with upper and lower cavities connected. Alumina nanoporous membranes (Whatman, Inc., UK.) were treated in boiling hydrogen peroxide for 30 minutes, washed and dried with deionized water, and then immersed in 3-(2,3-glycidyloxy)propyltrimethoxy In a mixed solution of 3-(2,3-glycidoxy)propyltrimethoxysilane (Sigma Aldrich) and toluene (Sigma Aldrich), the mass fraction of 3-(2,3-glycidoxy)propyltrimethoxysilane was 2%, and stood at 60°C for 20 hours After silanization treatment...

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Abstract

The invention relates to a preparation method for an electrochemical histamine sensor based on nano-porous film-magnetic nano-particles. The preparation method includes the steps: taking aluminum oxide nano-porous films as core sensing chips; treating the aluminum oxide nano-porous films by the aid of 3-(2,3-propylene oxide) GPMS (3-glycidoxypropyltrimethoxysilane); directly fixing histamine antibodies; sealing by the aid of PBS (poly butylenes succinate) buffer solution of bovine serum albumin with the mass concentration of 1%; fixing the antibodies by Fe3O4 magnetic nano-particles with carboxylated surfaces through EDC / NHS; enriching histamine in samples into the aluminum oxide nano-porous films treated by biology functionalization; blocking nano-pores; detecting the nano-pores by the aid of an electrochemical impedance analysis method. According to the preparation method, histamine can be rapidly detected, and the sensor is high in detection sensitivity, low in lower detection limit and high in specificity.

Description

(1) Technical field [0001] The invention relates to a preparation method of an electrochemical histamine sensor based on nanoporous membrane-magnetic nanoparticles. (2) Background technology [0002] Fresh seafood, such as saury, mackerel, tuna, etc. are rich in histidine. Seafood stored for too long is prone to spoilage and breeds bacteria, which decarbonylates histidine to form biogenic amines such as histamine. Eating spoiled seafood and excessive intake of histamine may damage the nervous system and cardiovascular system. Therefore, histamine toxicity is an important issue affecting food safety and public health. [0003] The main challenge in preventing histamine poisoning is that it is difficult to judge histamine accumulation from the surface of seafood. Once histamine is produced in fish, histamine has good thermal stability, and it is difficult to remove it by heating and extending the heating time. Therefore, it is necessary to develop various detection techniq...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/416G01N33/531G01N33/543
CPCG01N27/416G01N33/531G01N33/54326G01N33/54346
Inventor 叶伟伟徐一帆郑力豪张宇孙毅
Owner ZHEJIANG UNIV OF TECH