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Water-in-oil-in-water type vaccine adjuvant and application thereof

A water-in-oil-in-water vaccine adjuvant technology, which is applied in the field of water-in-oil-in-water vaccine adjuvants, can solve problems such as difficulty in vaccine injection, improve immune efficacy and cellular immunity, achieve good absorption, and reasonably controlled release Effect

Inactive Publication Date: 2017-03-22
天津三江永利生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the technical defects of the prior art, and provides a water-in-oil-in-water vaccine adjuvant and its application, so as to solve the technical problem that the vaccine prepared by the vaccine adjuvant of the prior art is difficult to inject

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A water-in-oil-in-water vaccine adjuvant, consisting of the following components: 3.5g of lauryl glucoside, 3.7g of polyethylene glycol monooleate, 2.0g of fatty acid methyl ester, 2.8g of glyceryl monostearate, White Oil VACCUTE-L88g, 1,2-Propanediol 1g, Glucose Glycine 1g.

[0035] The above components are mixed in sequence to prepare a stable water-in-oil-in-water vaccine adjuvant. The above-mentioned adjuvants were divided into 5 parts on average, and the inactivated Newcastle disease vaccine of the water-in-oil-in-water type, the inactivated vaccine of infectious bursal virus of the water-in-oil-in-water type, and the porcine ring of the water-in-oil-in-water type were prepared respectively. Inactivated virus vaccine, inactivated vaccine of porcine Japanese encephalitis virus in water-in-oil-in-water type.

[0036] The above five types of vaccines are all milky white stable emulsions, stored at 37°C for 35 days, centrifuged at 6000rpm for 15 minutes, and no water ...

Embodiment 2

[0042] A water-in-oil-in-water vaccine adjuvant, consisting of the following components: 4.0g lauryl glucoside, 3.92g polyethylene glycol monooleate, 3.0g fatty acid methyl ester, 2.28g glyceryl monostearate, White oil VACCUTE-L 85g, 1,2-propanediol 0.5g, Span 80 1.3g.

[0043] The above components are mixed in sequence to prepare a stable water-in-oil-in-water vaccine adjuvant. The above-mentioned adjuvants were divided into 5 parts on average, and the inactivated Newcastle disease vaccine of the water-in-oil-in-water type, the inactivated vaccine of infectious bursal virus of the water-in-oil-in-water type, and the porcine ring of the water-in-oil-in-water type were prepared respectively. Inactivated virus vaccine, inactivated vaccine of porcine Japanese encephalitis virus in water-in-oil-in-water type.

[0044] The above five types of vaccines are all milky white stable emulsions, stored at 37°C for 35 days, centrifuged at 6000rpm for 15 minutes, and no water phase precipi...

Embodiment 3

[0050] A water-in-oil-in-water vaccine adjuvant, consisting of the following components: polyvinylpyrrolidone 2.8g, polyethylene glycol monooleate 3.2g, glyceryl monostearate 2.1g, polyethylene glycol dioleate 3.9g ester, 89g white oil VACCUTE-L, 0.5g 1,2-propanediol, 0.5g glucosylglycine.

[0051] The above components are mixed in sequence to prepare a stable water-in-oil-in-water vaccine adjuvant. The above-mentioned adjuvants were divided into 5 parts on average, and the inactivated Newcastle disease vaccine of the water-in-oil-in-water type, the inactivated vaccine of infectious bursal virus of the water-in-oil-in-water type, and the porcine ring of the water-in-oil-in-water type were prepared respectively. Inactivated virus vaccine, inactivated vaccine of porcine Japanese encephalitis virus in water-in-oil-in-water type.

[0052] The above five types of vaccines are all milky white stable emulsions, stored at 37°C for 35 days, centrifuged at 6000rpm for 15 minutes, and n...

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Abstract

The invention provides a water-in-oil-water type vaccine adjuvant and application thereof. According to the technical scheme, ingredients which conform to large production demands and have immunopotentiation are preferred, according to a controlled release principle of a water-in-oil-water type microsphere, an adding mode is designed, thus the water-in-oil-water type vaccine adjuvant more effectively directly acts on an immune cell, and the immune potency is improved. Compared with an regular adjuvant in the prior art, the prepared water-in-oil-water type vaccine adjuvant has reasonable controlled release, target and an isolation characteristic, and after poultry is injected with a vaccine, the water-in-oil-water type microsphere timely metabolizes and breaks so as to comprehensively motivate a poultry lymphocyte to generate an antibody. An immune reinforcing agent with a heavy water phase can be lastingly and slowly release in a subsequent immune cell infiltration process to make bioavailability of the immune reinforcing agent maximize, it is shown that an injected part is well absorbed, an injected animal fast generates immunoprotection, and not only short-term immunity can be induced, but also long-term immunity can be induced.

Description

technical field [0001] The invention relates to the technical field of vaccines, in particular to a water-in-oil-in-water vaccine adjuvant and its application. Background technique [0002] Vaccines are preventive biological products that are immunogenic but do not cause disease in the body. The antigenic substances of vaccines are mostly taken from the inactivated or attenuated products of pathogenic microorganisms. When the inactivated antigen is immunized with animals alone, it is not enough to produce immunogenicity, especially the antigen with low immunogenicity and small molecular weight. Therefore, it is necessary to use different types of adjuvants to enhance the body's immune response to antigens. [0003] Studies have confirmed that in the process of vaccine preparation, adjuvants, as substances that can enhance immunogenicity or change the type of immune response together with antigens or pre-injected into the body, are favored by researchers in the development o...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61P37/04A61K39/17A61K39/12A61P31/14
CPCA61K39/39A61K39/12A61K2039/5252A61K2039/552A61K2039/55511A61K2039/55583C12N2720/10034C12N2750/10034C12N2760/18134C12N2770/24134A61K2300/00
Inventor 米娜张忠江刘恒权孟莎莎刘金秋张心原
Owner 天津三江永利生物科技有限公司
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