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Method for producing lysine and feed

A lysine and feed technology, applied in the field of amino acid preparation, can solve the problem of high cost of fermentation medium, achieve the effects of improving fermentation efficiency, strong synergy, and reducing production costs

Inactive Publication Date: 2017-03-29
CHANGSHA POWERLIFE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cost of fermentation medium is currently high, and many companies that produce amino acids are facing losses. How to reduce the cost of fermentation is a problem that companies need to solve

Method used

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Examples

Experimental program
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Embodiment 1

[0027] A kind of technique of fermentative production lysine hydrochloride, it comprises the steps:

[0028] ① Preparation of culture medium and fermentation

[0029] Corynebacterium glutamicum ATCC14997, Brevibacterium flavum ATCC14067 and Brevibacterium lactofermentum ATCC13869 were selected as fermentation strains; the three bacterial solutions were mixed according to the volume ratio of 1:1:1, and the concentrations of the three bacterial solutions were all 1×116 / mL, the mixed bacterial solution was inserted into the seed medium, the inoculation amount was 10%, and it was cultivated to the logarithmic phase to obtain the seed solution.

[0030] Among them, the seed medium preparation method: concentration 50% glucose solution 2000kg, yeast powder 140kg, corn steep liquor 420kg, MgSO 4 35kg, KH 2 PO 4 63kg, 9.1kg of p-aminobenzoic acid, 35kg of ammonium sulfate, 70kg of ferrous sulfate heptahydrate, and sterilized at 120°C for 15 minutes.

[0031] Inoculate the seed li...

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Abstract

The invention belongs to the technical field of amino acid preparation, and particularly relates to a method for producing lysine and feed. The method comprises the following steps of (1) preparing a culture medium and performing fermentation: inoculating 10% of mixed bacterial liquid into a seed culture medium, and performing culture to prepare seed liquid; inoculating 10% of the seed liquid to a fermentation culture medium, and performing aerobic fermentation to prepare fermented liquid; (2) performing filtration sterilization: performing filtration sterilization on the fermented liquid through an ultrafiltration membrane, and collecting concentrated liquid and penetrating liquid; (3) preparing feed: adding the concentrated liquid obtained in the step (2) to pre-prepared feed, and performing stirring to obtain the lysine and the feed; and (4) extracting the lysine: performing decolorization, concentration and crystallization on the penetrating liquid obtained in the step (2) to obtain the lysine. According to the method, mixed fermentation strains are mixed reasonably, so that the synergistic effect is good and the fermentation efficiency can be greatly improved; and the lysine and the high-protein feed product are produced at the same time, so that the production cost is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of amino acid preparation, and in particular relates to a method for producing lysine and feed. Background technique [0002] Lysine plays an important physiological function in the body, and its deficiency will directly or indirectly affect the growth, reproduction and development of animals. Lysine is an indispensable component of synthetic body protein, plays a very important role in the formation of enzyme protein, germ cells, skeletal muscle and hemoglobin, and is also one of the components of certain polypeptide hormones. [0003] Existing forms of lysine Lysine has two isomers, L-form and D-form. The active ingredient content of L-lysine is generally 77%~79%. Monogastric animals are completely unable to synthesize lysine by themselves and do not participate in transamination. After the amino group of D-amino acid and L-amino acid is acetylated, it can be deaminated by D-amino acid oxidase or L-amin...

Claims

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Application Information

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IPC IPC(8): C12P13/08A23K20/142A23K10/37C12R1/15C12R1/13
CPCC12P13/08Y02P60/87
Inventor 刘继根
Owner CHANGSHA POWERLIFE BIOTECH
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