A method for detecting Salmonella in textiles
A technology for salmonella and textiles, applied in the field of salmonella, can solve the problems of cumbersome procedures, long cycle, low sensitivity, etc.
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Embodiment 1
[0092] A method for rapidly detecting salmonella in textiles, comprising the steps of:
[0093] Step 1: Sample enrichment
[0094] Aseptically open the textile sample for inspection, cut the sample evenly with sterile scissors, accurately weigh 25g of the cut sample on an electronic balance, cut it into pieces and add it to a sterile homogeneous bag containing 225mL of SCDLP liquid medium. Beat with a slap-type homogenizer for 1min to 2min, and mix thoroughly; put the prepared sample into a constant temperature incubator at 36°C±1°C, and enrich the bacteria for 16h±2h to obtain the first enrichment solution;
[0095] Step 2: LAMP test
[0096] 2.1) Preparation of DNA template: Take 1mL of SCDLP enrichment solution and put it into a 1.5mL sterile Eppendorf tube, centrifuge at 10000r / min in a high-speed refrigerated centrifuge for 5min, discard the supernatant; add 1mL of sterilized double-distilled water, mix well, and centrifuge in a high-speed refrigerated centrifuge Centri...
Embodiment 2
[0113] A method for rapidly detecting salmonella in textiles, comprising the steps of:
[0114] Step 1: Sample enrichment
[0115] Aseptically open the textile sample for inspection, cut the sample evenly with sterile scissors, accurately weigh 25g of the cut sample on an electronic balance, cut it into pieces and add it to a sterile homogeneous bag containing 225mL of SCDLP liquid medium. Beat with a slap-type homogenizer for 1min to 2min, and mix thoroughly; put the prepared sample into a constant temperature incubator at 36°C±1°C, and enrich the bacteria for 16h±2h to obtain the first enrichment solution;
[0116] Step 2: LAMP test
[0117] 2.1) Preparation of DNA template: Take 1mL of SCDLP enrichment solution and put it into a 1.5mL sterile Eppendorf tube, centrifuge at 10000r / min in a high-speed refrigerated centrifuge for 5min, discard the supernatant; add 1mL of sterilized double-distilled water, mix well, and centrifuge in a high-speed refrigerated centrifuge Centri...
Embodiment 3
[0134] A method for rapidly detecting salmonella in textiles, comprising the steps of:
[0135] Step 1: Sample enrichment
[0136] Open the textile sample submitted for inspection by aseptic method, cut the sample evenly with sterile scissors, accurately weigh 25g of the cut sample on the electronic balance, cut it into pieces and add it to a sterile homogeneous bag containing 225mL of SCDLP liquid medium. Beat with a slap-type homogenizer for 1 to 2 minutes, and mix thoroughly; put the prepared sample into a constant temperature incubator at 36°C±1°C, and enrich the bacteria for 16h±2h to obtain the first enrichment solution;
[0137] Step 2: LAMP test
[0138] 2.1) Preparation of DNA template: Take 1mL of SCDLP enrichment solution and put it into a 1.5mL sterile Eppendorf tube, centrifuge at 10000r / min in a high-speed refrigerated centrifuge for 5min, discard the supernatant; add 1mL of sterilized double-distilled water, mix well, and centrifuge in a high-speed refrigerated...
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