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High-sensitivity biosensor produced via nanoparticle technology and production method thereof

A biosensor and high-sensitivity technology, which is applied in the field of high-sensitivity biosensors and its preparation, can solve the problems of reducing sensor sensitivity, consuming large samples, and high oxidation potential, and achieve the effects of eliminating adverse effects, reducing detection time, and increasing sensitivity

Inactive Publication Date: 2017-03-29
HONG KONG PRODUCTIVITY COUNCIL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the direct amperometric electrochemical detection of hydrogen peroxide requires a relatively high oxidation potential, so the experimental results may be interfered by easily oxidized species, which will reduce the sensitivity of the sensor and require a large amount of sample (more than 1 μl )

Method used

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  • High-sensitivity biosensor produced via nanoparticle technology and production method thereof
  • High-sensitivity biosensor produced via nanoparticle technology and production method thereof
  • High-sensitivity biosensor produced via nanoparticle technology and production method thereof

Examples

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preparation example Construction

[0037] The invention provides a kind of high-sensitivity biosensor prepared by means of nanoparticle technology and a preparation method thereof, based on the method, the biosensor is prepared as a nanoparticle-enzyme biosensor microchip, such as figure 1 , figure 2 , image 3 As shown, the high-sensitivity biosensor consists of a plastic substrate 1, and the conductive electrode layer 2, insulating layer 3 and nanoparticle layer 4 on its surface can be deposited by screen printing technology using corresponding ink and chemical substances respectively. The specific preparation process of the high-sensitivity biosensor is as follows: Figure 4 As shown, firstly, two electrodes are screen-printed on the surface of the plastic substrate, one of which is the working electrode and the other is the counter electrode, forming a conductive metal or non-metal electrode layer, and the two electrodes are separated by a small gap. Then print a layer of non-conductive insulating layer ...

Embodiment 1

[0064] This embodiment provides a method for preparing a high-sensitivity biosensor by means of nanoparticle technology, which specifically includes the following steps:

[0065] Step 1: Pretreatment of the plastic substrate, before pretreatment, cut a polyvinyl chloride (PVC) substrate with a size of 1500 mm × 1500 mm into 18 cm × 10 cm. Place the PVC base plate between two symmetrical stainless steel plates and press from both sides. The polyvinyl chloride substrate together with the stainless steel plate was placed in a 70°C oven for 30 minutes and allowed to cool in the atmosphere. The operation was repeated so that a flat polyvinyl chloride substrate was obtained and thermal stress was removed to prevent the substrate from warping. Remove the stainless steel plate from the PVC substrate.

[0066] Step 2: Weigh a sufficient amount of conductive ink and mix it with the corresponding solvent (if any), such as carbon paste, silver and palladium can be mixed with thinner, re...

Embodiment 2

[0071] This implementation provides a method for preparing a high-sensitivity biosensor by means of nanoparticle technology, which specifically includes the following steps:

[0072] Step 1: Pretreat the plastic substrate, which is made of polyester sheet (PET), with a size of 210mm×297mm. Before pretreatment, cut the polyester sheet substrate into a suitable size of 18cm×10cm. The PET substrate was placed between two symmetrical stainless steel plates and pressed from both sides. The polyester sheet substrate together with the stainless steel plate was placed in a 90°C oven for 30 minutes and allowed to cool in the atmosphere. Repeat the above operations in order to obtain a flat polyester sheet substrate and remove thermal stress to prevent the substrate from bending. Remove the stainless steel plate from the polyester sheet substrate.

[0073] Step 2: Weigh a sufficient amount of conductive ink and mix it with the corresponding solvent (if any), such as carbon paste, silv...

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Abstract

The invention provides a high-sensitivity biosensor produced via nanoparticle technology and a production method thereof; the production method comprises the steps of pretreating a plastic baseplate; depositing a conductive electrode layer via silkscreen technology; depositing an insulating layer via silkscreen technology; producing nanoparticle solution, depositing via silkscreen technology, producing enzyme solution, and dip-coating. Compared with biosensor microchips, the biosensor of the invention has increased adsorption surface area via nanoparticles such that it is possible to achieve detection with just few samples, detection time is saved, and biomass detection sensitivity of the biosensor is also improved; by using nanoparticles to form an interface layer, the disturbance of electroactive materials and oxygen is effectively decreased. Compared with the traditional production methods, the silkscreen technology for producing the nanoparticle-based enzyme biosensor has the advantages of low cost, high stability and rich functionality.

Description

technical field [0001] The invention belongs to the technical field of biosensor coating, and in particular relates to a high-sensitivity biosensor prepared by means of nanoparticle technology and a preparation method thereof. Background technique [0002] The market for biosensors is growing rapidly owing to the increasing geriatric population and the increasing number of people suffering from chronic diseases such as type 2 diabetes. It is estimated that by the end of 2015, the total global market for biosensors will reach 12 billion US dollars. Among them, glucose biosensors occupy the largest market share (about 80%). The market share of other commonly used biosensors, such as cholesterol and blood gas sensors, will also grow rapidly. Frequent detection of the level of biological substances (such as blood sugar, cholesterol) is very important for the early diagnosis of chronic diseases and the establishment of relatively effective treatment options. Enzyme-based (eg, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 卢伟贤潘志豪郑颕怡
Owner HONG KONG PRODUCTIVITY COUNCIL
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