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Amplimers for mitochondrial COI gene of Bellamya and application thereof

A technology for gene amplification and Cyclopyridium, which is applied in the field of benthic animal identification and classification, can solve problems such as the design of species-specific primers and the evaluation of sequencing results that have not been seen yet, and meets the requirements of simple operation and equipment. low effect

Inactive Publication Date: 2017-04-12
HUAZHONG AGRI UNIV
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Problems solved by technology

[0006] So far, there have been no reports on the design of Pyramidrospirus species-specific primers and the evaluation of sequencing results

Method used

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  • Amplimers for mitochondrial COI gene of Bellamya and application thereof
  • Amplimers for mitochondrial COI gene of Bellamya and application thereof
  • Amplimers for mitochondrial COI gene of Bellamya and application thereof

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Experimental program
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Embodiment Construction

[0023] The present invention will be described in detail below in conjunction with examples.

[0024] Embodiments of the present invention are carried out in the Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, Fisheries College, Huazhong Agricultural University, and the specific implementation steps are as follows:

[0025] (1) Collect a number of live snails in Liangzi Lake, Ezhou City, Hubei Province, and select some individuals with typical external morphological characteristics that are easy to pass morphological identification and individuals with insignificant external morphological characteristics that are difficult to pass morphological identification, a total of 30 individuals The sample, the shape of the sample of the snail is as follows figure 1 shown.

[0026] (2) Scrub the external sludge and attached algae of the snail shell, take pictures for archiving, and use sharp tweezers to pick up the muscle tissue of the g...

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Abstract

The invention specifically relates to amplimers for a mitochondrial COI gene of Bellamya and application thereof, belonging to the technical field of identification and classification of zoobenthos. The invention is characterized in that specific primers are designed according to the COI gene sequences of a part of species belonging to a same subclass and amplification verification is carried out; and an application method for the amplimers comprises the following main steps: (1) extracting the total DNAs of tissue of Bellamya samples; (2) based on the complete mitochondrial COI sequence of Mollusca, Prosobranchia, published in the NCBI nucleic acid database (http: / / www.ncbi.nlm.nih.gov / nuccore), carrying out comparative analysis on the total DNAs by using Cluster W2, designing the specific primers and carrying out synthesis; (3) subjecting the COI genes of to-be-identified Bellamya individuals to amplification via the primers by using a standard PCR system according to a standard PCR procedure; and (4) optimizing splicing and sequencing results and comparing the sequencing results in the NCBI nucleic acid database via BLAST.

Description

technical field [0001] The invention belongs to the technical field of identification and classification of benthic animals. In particular, it relates to a primer for amplifying the mitochondrial COI gene of the spirulina and its application. Background technique [0002] Species identification and classification are the basic conditions for biological research. It is estimated that there are about 5 million to 100 million living species on the earth, but currently only about 1.7 million species can be identified by taxonomists using traditional classification methods. Today the world is facing many serious problems such as maintaining genetic diversity, biological species diversity and avoiding the occurrence of epidemics, and the premise of solving these problems is to be able to correctly identify and classify biological species (Frezal & Leblois, 2008). However, species identification and classification is a daunting task for the vast population of organisms on Earth. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888
Inventor 王炬光王卫民
Owner HUAZHONG AGRI UNIV
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