Immune colloidal gold detection card of chlorothalonil, and preparation method thereof
A technology of colloidal gold and chlorothalonil, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of expensive equipment, complicated operation, difficult to popularize, etc., and achieve the effect of simple method and accurate results.
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Embodiment 1
[0020] figure 1 , figure 2 Example shown: 9 is a PVC rubber sheet in the figure; 7 is a sample pad; 8 is a colloidal gold binding pad coated with a monoclonal antibody colloidal gold marker; 10 is a coating film, namely nitrocellulose The nitrocellulose membrane is coated with chlorothalonil-BSA and goat anti-mouse IgG; 11 is an absorbent pad made of absorbent material such as filter paper.
[0021] The sample pad 7 and the bonding pad 8 are adhered to one end (sample end) of the PVC rubber sheet 9, and the sample pad 7 and the bonding pad 8 are in a side-by-side structure.
[0022] The nitrocellulose film 10 is adhered to the middle of the PVC rubber sheet 9. A goat anti-mouse IgG quality control line 3 and a chlorothalonil-BSA detection line 2 are set on the nitrocellulose membrane 10.
[0023] Adhere the absorbent pad 11 to the other end of the PVC rubber sheet 9. One end of the nitrocellulose membrane 10 slightly crosses the bonding pad 8 and the other end slightly crosses th...
Embodiment 2
[0025] The preparation of the chlorothalonil colloidal gold test card is implemented by the following steps:
[0026] Preparation of colloidal gold solution: Take one 1 L Erlenmeyer flask, add 495 mL ultrapure water, and then add 1% chloroauric acid (HAuCl 4 ·3H 2 O) 5 mL, prepared into 500 mL 0.01% chloroauric acid aqueous solution, heated and boiled, add 1% trisodium citrate (Na 3 C 6 H 5 O 7 ·2H 2 O) 5-7 mL of the solution, continue to stir and heat, when the color of the solution completely changes to a transparent purple-red, keep it for 5 minutes, stop heating, add water to the original volume, cool to room temperature, and store at 2-8°C for later use;
[0027] Antibody pretreatment: Centrifuge the chlorothalonil antibody to be labeled at 1000 r / min, 4℃ for 20 min, take the supernatant, and dilute it with 0.01 mol / L PBS to 1 mg / mL; or use 0.01 mol / L Dilute PBS to 1 mg / ml, and pass through 0.22 µm filter membrane;
[0028] Preparation of the colloidal gold marker: Take 40 mL of...
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