High-seedling-rate culture method for tissue culture of Polygala fallax stems

A cultivation method and technology of seedling success rate, applied in the field of propagation of Polygala chrysogenum seedlings, can solve problems such as rapid propagation and popularization of unfavorable good varieties, no test-tube seedling transplanting management, and easy variation of offspring seedlings, etc., to achieve rapid Effects of reproduction, prevention of vitrification and callus, and avoidance of basal callus

Active Publication Date: 2017-04-26
福建省农业科学院农业生物资源研究所
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  • Summary
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Problems solved by technology

[0004] The research on tissue culture of Polygala chrysalis has been applied for an invention patent "A Method for Tissue Culture and Propagation of P. chinensis (Application No. 201210147606.9)", and the research paper "Research on Tissue Culture and Rapid Propagation of P. chrysalis" published by Liu Xiufang et al. , the above literatures all use young stems with axillary buds as explants, and adopt the approach of adventitious bud proliferation for rapid propagation. During the cultivation process, adventitious buds are prone to vitrification, adventitious bud bases and callus,

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specific Embodiment approach

[0047] The method for cultivating high seedling rate through tissue culture of Polygala chrysanthemum stem section comprises the following steps:

[0048] (1) Collection and disinfection of explants: collection of semi-lignified branches with ungerminated axillary buds of Polygala chrysanthemum, removing leaves, cutting at least one internode stem segment as explants, and disinfecting it;

[0049] (2) Obtain axillary buds by inducing culture: cut off the two ends of the above-mentioned sterilized explants in contact with the disinfectant, leave a stem section with at least one internode, and then obliquely cut the morphological lower end of the stem section Insert on the axillary bud induction medium that induces axillary bud germination to carry out axillary bud induction culture for 15-20 days,

[0050] Axillary bud induction culture conditions are: temperature 20±2°C, dark conditions without light;

[0051] Through the above treatment, invisible axillary buds germinate and...

example 1

[0076](1) Collection and disinfection of explants: On sunny days for 5 consecutive days, without watering the day before, cut the semi-lignified branches of the chrysanthemum chrysalis with ungerminated axillary buds, remove the leaves, and cut the segmented stems as explants. For the plant, use alcohol cotton balls with a mass concentration of 75% to wipe the stem explants for 2 times, then place them on the ultra-clean workbench, cut them into stems with 1 internode, and use 0.1% carbon dioxide Soak in chlorine for 12 minutes, rinse with sterile water twice, and store in a dry sterile bottle.

[0077] (2) Obtain axillary buds by inducing culture: clamp the above-mentioned sterilized aseptic explants with sterile tweezers, cut off the parts that contact the disinfectant at both ends, and leave a 1.0 cm long stem segment with 1 internode. The lower end of the morphology was obliquely inserted on the solid medium for inducing the germination of axillary buds, and placed in a da...

example 2

[0086] (1) Collection and disinfection of explants: On sunny days for 5 consecutive days, without watering the day before, cut the semi-lignified branches of the chrysanthemum chrysalis with ungerminated axillary buds, remove the leaves, and cut the segmented stems as explants. For the plant, use alcohol cotton balls with a mass concentration of 75% to wipe the stem explants for 2 times, then place them on the ultra-clean workbench, cut them into stems with 1 internode, and use 0.1% carbon dioxide Soak in chlorine for 20 minutes, rinse with sterile water three times, and store in a dry sterile bottle.

[0087] (2) Obtain axillary buds by inducing culture: clamp the above-mentioned sterilized aseptic explants with sterile tweezers, cut off the parts that contact the disinfectant at both ends, and leave a 1.0 cm long stem segment with 1 internode. The lower end of the morphology was obliquely inserted on the solid medium for inducing the germination of axillary buds, and placed ...

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Abstract

The invention relates to a high-seedling-rate culture method for tissue culture of Polygala fallax stems. The method comprises steps as follows: explants are collected and disinfected, axillary buds are obtained through induced culture, strong stems are obtained through strong seedling culture, tissue culture stems are obtained through subculture proliferation, induced root treated stems are obtained through root induction treatment and rooting is performed outside test tubes. Semi-lignified branches with axillary buds not germinated yet are adopted, leaves are removed, and branches at least comprising one internode stem are cut to serve as the explants. With the adoption of the steps, formation of taproots of Polygala fallax seedlings is facilitated, the seedling quality is improved, the yield of the Polygala fallax medicinal material is increased, descendant seedlings cannot vary easily, and the method has very great significance in culture and medicinal material production of the Polygala fallax seedlings.

Description

technical field [0001] The invention relates to a method for propagating Polygala chrysalis seedlings, in particular to a method for cultivating Polygala chrysogenum stem segments with high seedling rate through tissue culture, and belongs to the technical field of Polygala chrysogenum seedling cultivation methods. Background technique [0002] Polygala fallax (Polygala fallax Hemsl.) belongs to Polygalaceae (Polygalaceae) Polygala plant, aliased with inverted yellow, yellow ginseng, chicken tree, Guanyin string, false yellow Polygala, etc., distributed in Jiangxi, Fujian, Hunan, Guangdong , Guangxi and Yunnan, born in the damp place next to the water under the forest in the valley. Its root contains saponins, glycolipids, ketones and other substances. The dried root is a common folk medicine with a long history of application. Soreness, bruises, jaundice, edema, irregular menstruation, etc. In addition, the inflorescence of Polygala chrysalis is inverted, bright yellow, 2...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈菁瑛黄颖桢赵云青刘保财
Owner 福建省农业科学院农业生物资源研究所
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