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Synthetic method and application of hapten of tetrabromobisphenol A derivative (TBBPA-MHEE)

A technology of tetrabromobisphenol and a synthesis method, which is applied in the field, can solve the problems of the specificity and polarity of the analyte, and achieve the effects of strong specificity and high antibody titer.

Inactive Publication Date: 2017-04-26
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the analyte molecules do not contain functional groups, or the functional groups have a great influence on the specificity and polarity of the analyte. A functional group on the carrier protein (such as amino, carboxyl, hydroxyl and sulfhydryl, etc.)

Method used

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  • Synthetic method and application of hapten of tetrabromobisphenol A derivative (TBBPA-MHEE)
  • Synthetic method and application of hapten of tetrabromobisphenol A derivative (TBBPA-MHEE)

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Embodiment Construction

[0023] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0024] (1) Use TBBPA as raw material to generate methyl-2-(2,6-dibromo-4-(2-(3,5-dibromo-4-( 2-Hydroxyethoxy)phenyl)propan-2-yl)phenoxy)ethyl acetate (d);

[0025] Add K in 10ml of DMF dissolved in TBBPA (1.08g, 0.002mol) 2 CO 3 (0.828g, 0.006mol), methyl bromoacetate (0.306g, 0.002mol) was added dropwise at 90°C, and stirring was continued for 12h. 2-Bromoethanol (0.242 g, 0.002 mol) was added dropwise to the reaction solution to react for 4 h. After washing with water, the organic phase was extracted three times with 20 mL of ethyl acetate, concentrated and purified to obtain d with a yield o...

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Abstract

The invention provides a synthetic method and application of a hapten of a tetrabromobisphenol A derivative (TBBPA-MHEE). The synthetic method comprises the following steps: (1) with TBBPA as a raw material, subjecting TBBPA, methyl bromoacetate and 2-bromoethanol to a substitution reaction so as to produce methyl-2-(2,6-dibromo-4-(2-(3,5-dibromo-4-(2-hydroxyethyoxy)phenyl)prop-2-yl)phenoxy)ethyl acetate; and (2) subjecting a reaction product of the previous step to saponification with sodium hydroxide and acidification with hydrochloric acid so as to obtain the hapten 2-(2,6-dibromo-4-(2-(3,5-dibromo-4-(2-hydroxyethyoxy)phenyl)prop-2-yl)phenoxy)acetic acid. According to the invention, the hapten of the tetrabromobisphenol A derivative (TBBPA-MHEE) is successfully designed and synthesized through repeated experiments; and the synthetic method provided by the invention is a novel synthetic method for the hapten and is simple, safe and practicable.

Description

technical field [0001] The invention belongs to the technical field of tetrabromobisphenol A derivative TBBPA-MHEE hapten, in particular to a synthesis method of tetrabromobisphenol A derivative TBBPA-MHEE hapten, referring to a tetrabromobisphenol A derivative TBBPA-MHEE One of the side chains of the benzene ring is modified so that it has a synthesis method for coupling carboxyl groups, specifically a synthesis method of a tetrabromobisphenol A derivative TBBPA-MHEE hapten and its use. Background technique [0002] Immunoassay is an analysis method based on antigen and antibody specific reaction, which has been widely used in the analysis and detection of small molecule pollutants in food and environment. This method has the advantages of high sensitivity, convenience, high throughput, and low cost. The premise of establishing this method is the development of corresponding antibodies. Due to their small molecular weight and lack of immunogenicity, small molecular compou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C27/02C07C59/70
CPCC07C51/09C07C67/31C07C59/70C07C69/712
Inventor 张祯梁亚如曾昆吴向阳张萌黄梦璐
Owner JIANGSU UNIV