P-hydroxyphenyl propanoic acid extracted from white mulberry root barks as well as preparation method and application thereof

Abstract

The invention relates to p-hydroxyphenyl propanoic acid extracted from white mulberry root barks as well as a preparation method and an application thereof to effectively solve the problems of extracting the p-hydroxyphenyl propanoic acid from the white mulberry root barks and applying the p- hydroxyphenyl propanoic acid to preparation of medicines for treating LPS (lipopolysaccharide) induced HPAEC (Human Pulmonary Artery Endothelial Cells) injuries. The preparation method comprises the following steps: performing decoction extraction on the white mulberry root barks; performing vacuum concentration on an extract to obtain a dried product; adding distilled water into the dried product to perform suspension; performing treatment on a resin column; performing gradient elution by using water and ethanol in sequence; concentrating eluant of each gradient until the eluant is dried; dissolving the dried product by using methanol; adding silica gel; adding the mixture to the liquid surface of a chromatographic column; performing gradient elution; performing TLC (thin layer chromatography) analysis and combination on each fraction; performing vacuum concentration to obtain gross samples A1, A2 and A3; and dissolving the gross sample A1 by using methanol, performing treatment on a Toyopearl HW-40 column, performing elution by using methanol, performing thin layer detection and combination to obtain a fraction A1-2, performing vacuum concentration and drying, performing dissolution by using methanol, performing treatment on an ODS (octadecylsilyl) column, performing elution by using methanol, performing thin layer detection, combining parts containing the same fractions, and performing vacuum concentration to obtain the p-hydroxyphenyl propanoic acid. The method provided by the invention is novel and unique, is easy to operate and use, and opens up a new application of the white mulberry root barks.

Description

technical field [0001] The invention relates to medicine, in particular to p-hydroxyphenylpropionic acid extracted from Cortex Mori and its preparation method and application. Background technique [0002] Morus alba is the dry root bark of the Moraceae plant mulberry (Latin scientific name: Morus alba L.), also known as mulberry root white bark, mulberry root bark, mulberry bark, and white mulberry bark. , scrape off the yellow-brown rough bark, cut it longitudinally, peel off the root bark, and dry it in the sun. Contains flavonoids: mulberrin, mulberrochromene, cyclomulberrin, cyclomulberro-chromene, morusin, cyclomulberry Cyclomorusin, oxydihydromomomsin, kuwanon A, B, C, D, E, F, G (ie albanin F, moracenin B), H (ie albanin G, moracenin A), I, K, L, Y, Z, moracenin C, D, sffnggenone A~P; and mulberrofuran A, B, C, K, N , 0, M, P, Q, betulinic acid; coumarins: 5,7-dihydmxycoumarin (5,7-dihydmxycoumarin), umbelliferone, scopolamine Elements, scopoletin), scopoletin an...

AI Technical Summary

Problems solved by technology

But so far, there is no application of extracting the effective active part p-hydroxyphenylpropionic acid (SP-6) from Morus alba and preparing it for the treatment of HPAEC damage induced by LPS.

Images