P-hydroxyphenyl propanoic acid extracted from white mulberry root barks as well as preparation method and application thereof
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A technology of p-hydroxyphenylpropionic acid and Morus alba, applied in the field of medicine, can solve the problems that have not been seen before, and achieve the effects of easy operation and use, novel and unique preparation method, good economic and social benefits
Inactive Publication Date: 2017-04-26
HENAN UNIV OF CHINESE MEDICINE
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But so far, there is no application of extracting the effective active part p-hydroxyphenylpropio
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[0022] The specific implementation of the present invention will be described in detail below in combination with specific situations.
[0023] In the specific implementation of the present invention, the described p-hydroxyphenylpropionic acid is an effective active ingredient extracted from Cortex Morus alba, and the molecular structure formula is shown in figure 1 Shown, the preparation method of this p-hydroxyphenylpropionic acid is:
[0024] Decoct 2.4 kg of Cortex Mori with 8-12 times the weight of distilled water and extract 3 times, each time for 60 minutes, combine the extracts, concentrate under reduced pressure to obtain a dry product, add distilled water 2-3 times the weight of the dry product and mix Suspension, weight volume refers to the solid matter in g, the liquid in mL, centrifuge at 6000r / min to remove the insoluble matter, and get the supernatant; take 7.0L Diaion HP-20 macroporous adsorption resin filler, soak it with 95% ethanol Pack the column for 24 h...
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Abstract
The invention relates to p-hydroxyphenyl propanoic acid extracted from white mulberry root barks as well as a preparation method and an application thereof to effectively solve the problems of extracting the p-hydroxyphenyl propanoic acid from the white mulberry root barks and applying the p- hydroxyphenyl propanoic acid to preparation of medicines for treating LPS (lipopolysaccharide) induced HPAEC (Human Pulmonary Artery Endothelial Cells) injuries. The preparation method comprises the following steps: performing decoction extraction on the white mulberry root barks; performing vacuum concentration on an extract to obtain a dried product; adding distilled water into the dried product to perform suspension; performing treatment on a resin column; performing gradient elution by using water and ethanol in sequence; concentrating eluant of each gradient until the eluant is dried; dissolving the dried product by using methanol; adding silica gel; adding the mixture to the liquid surface of a chromatographic column; performing gradient elution; performing TLC (thin layer chromatography) analysis and combination on each fraction; performing vacuum concentration to obtain gross samples A1, A2 and A3; and dissolving the gross sample A1 by using methanol, performing treatment on a Toyopearl HW-40 column, performing elution by using methanol, performing thin layer detection and combination to obtain a fraction A1-2, performing vacuum concentration and drying, performing dissolution by using methanol, performing treatment on an ODS (octadecylsilyl) column, performing elution by using methanol, performing thin layer detection, combining parts containing the same fractions, and performing vacuum concentration to obtain the p-hydroxyphenyl propanoic acid. The method provided by the invention is novel and unique, is easy to operate and use, and opens up a new application of the white mulberry root barks.
Description
technical field [0001] The invention relates to medicine, in particular to p-hydroxyphenylpropionic acid extracted from Cortex Mori and its preparation method and application. Background technique [0002] Morus alba is the dry root bark of the Moraceae plant mulberry (Latin scientific name: Morus alba L.), also known as mulberry root white bark, mulberry root bark, mulberry bark, and white mulberry bark. , scrape off the yellow-brown rough bark, cut it longitudinally, peel off the root bark, and dry it in the sun. Contains flavonoids: mulberrin, mulberrochromene, cyclomulberrin, cyclomulberro-chromene, morusin, cyclomulberry Cyclomorusin, oxydihydromomomsin, kuwanon A, B, C, D, E, F, G (ie albanin F, moracenin B), H (ie albanin G, moracenin A), I, K, L, Y, Z, moracenin C, D, sffnggenone A~P; and mulberrofuran A, B, C, K, N , 0, M, P, Q, betulinic acid; coumarins: 5,7-dihydmxycoumarin (5,7-dihydmxycoumarin), umbelliferone, scopolamine Elements, scopoletin), scopoletin an...
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