35 results about "P-hydroxyphenylpropionic acid" patented technology

The invention relates to a method for producing phloretin by fermentation of saccharomyces cerevisiae. The method is completed by performing catalysis on p-hydroxyphenylpropionic acid serving as a rawmaterial and the saccharomyces cerevisiae serving as host bacteria through a plurality of enzymes in the host bacteria. Compared with the prior art, the method disclosed by the invention is characterized in that (1) by taking a low-cost compound as the raw material, the technological raw material is low in cost; (2) on the technical basis of modification of microorganisms, catalytic synthesis ofbiological enzymes is carried out in the microorganisms, so that large-scale extraction, separation and purification processes are avoided, and the production cost and the environmental protection areeasy to control; (3) the scale and the quantity of production equipment in the process are less than the scale and the quantity in other methods, and industrial transformation is facilitated; (4) separation and purification procedures are only carried out in the last step of the production in the process, so that a product purification process is simple, the product quality is higher than that ina general method, and the content is higher; (5) no waste liquid is discharged in a synthesis process, so that the method is environmentally friendly and pollution-free and can realize sustainable production.

The invention relates to a new fluoroimmunoassay method for human thymidine kinase fluoroimmunoassay based on magnetic nanometer grains. The invention sets up a quick and simple immunological detecting technology for the high flux human thymidine kinase (hTK1), and the technology is used for the hTK1 clinical examination. The hTK1 is fixed with covalence on the surface of an amido silanization superparamagnetism nanometer grain immobilized carrier through glutaric dialdehyde, a competitive immunoassay method is adopted, horse radish peroxidase is used as an enzyme labeling, ethyl-para-hydroxyphenyl acid is used as a fluorogenic substrate, thereby realizing the detecting for the hTK1. The amido silanization nucleocapsid-shaped magnetic nanometer grains are evenly dispersed in liquidoid, thereby having the advantages of fixation and uniformity, high specificity, good repeatability, quick reaction velocity, etc., under the action of an adscititious magnetic field, the invention can effectively realize the separation and enrichment of the immune complex and the reaction liquid of the nucleocapsid-shaped magnetic nanometer grains, the operation of the analytical method is simple, accurate, sensitive and fast, automatic detection is easy to be realized, and large batch of test task can be completed.

The invention relates to a clenbuterol immunomagnetic bead separation and enrichment kit. The clenbuterol immunomagnetic bead separation and enrichment kit comprises a magnetic bead which is coupled to a clenbuterol monoclonal antibody, reconstitution fluid and a magnet, wherein the magnetic bead which is coupled to the clenbuterol monoclonal antibody is formed by mixing and dissolving the clenbuterol monoclonal antibody and the magnetic bead in 2-(N-morpholino) ethanesulfonic acid monohydrate in a coupling way according to a mass ratio of 1:(500 to 1000); the clenbuterol monoclonal antibody is obtained by taking a coupling substance obtained from clenbuterol haptin and bovine serum albumin as an immunogen to immune Balb/c mice; the clenbuterol haptin is obtained by carrying out diazo-reaction on clenbuterol and P-hydroxybenzene propanoic acid and bringing a carboxyl spacer arm on amino. The invention also relates to a sample preprocessing method for separating the clenbuterol by adopting the clenbuterol immunomagnetic bead separation and enrichment kit. According to the sample preprocessing method disclosed by the invention, higher specificity, higher recovery rate and higher accuracy on the clenbuterol are obtained, sample preprocessing steps are simplified, and various and a mass of organic solvents can be prevented from being used during a sample preprocessing procedure.

The invention discloses a preparation method of epsilon-polylysine-p-hydroxybenzene propanoic acid antibiotic hydrogel dressing. The method comprises the following steps: dissolving p-hydroxybenzene propanoic acid in a blended solvent of an organic solvent and deionized water, and stirring and uniformly mixing; adding 1-(3-dimethylamino propyl)-3-ethyl carbodiimide hydrochloride and N-hydroxysuccinimide, and performing activation in an ice-bath condition; adding epsilon-polylysine dissolved by deionized water into an activated system, so as to enable the epsilon-polylysine to react with the activated system at the room temperature; transferring an obtained system into a dialysis bag, so as to dialyze the system; freezing and drying dialyze purified solution, so as to obtain epsilon-polylysine-p-hydroxybenzene propanoic acid copolymer; respectively preparing a stock solution A and a stock solution B by taking PBS buffer solution as a solvent, respectively adding the stock solution A and the stock solution B into a tube A and a tube B of a double-end injector, and slowly pushing out the stock solution A and the stock solution B, so as to obtain the uniform and transparent epsilon-polylysine-p-hydroxybenzene propanoic acid antibiotic hydrogel dressing.

The invention provides a novel method for preparing an esmolol hydrochloride optical isomer, which comprises the following steps of: preparing alkoxide by adding an alkali into para hydroxybenzene methyl propionate in an anhydrous aprotic polar solvent; then carrying out condensation with the alkoxide and a (S)-glycidol derivative or a (R)-glycidol derivative to prepare an intermediate; preparing optically active esmolol hydrochloride by aminolysis of isopropyl amine and hydrogen chloride salifying; and refining the optically active esmolol hydrochloride to obtain (S)-esmolol hydrochloride or (R)-esmolol hydrochloride. The method has the advantages of simple and convenient operation, higher product purity and suitability for big industrial production.

The invention relates to a synthetic method of dimethoxy phosphate ester type pesticide universal hapten, and belongs to the technical field of the biochemical engineering. The synthetic process of the invention comprises three steps of esterification, acylation and hydrolysis. Firstly, para hydroxybenzene propionic acid and carbinol have esterification reaction to make carboxy metho esterified; and then the obtained para hydroxybenzene methyl propionate has reaction with dimethoxy phosphorus oxychloride to obtain dimethoxy phosphate ester; finally the methyl ester formed in the first step is hydrolyzed again, and carboxyl is reduced to obtain O, O-dimethyl-O-(4-propionyloxy phenyl group) phosphate ester, and the phosphate ester is coupled with egg albumin to be the envelope antigen separated out by redundant ELISA of the dimethoxy phosphate ester type pesticide. The substance is used as the envelope antigen during the process of enzyme linking immunity checking of the pesticide after being coupled with the albumen, and the structure has benzene ring, thereby using the difference of envelope antigen and the immunity primary structure to further improve the checking sensitivity and reduce the checking limitation.

The invention provides a technical method using 'photosynthetic bacteria', namely blue algae as the substrate organism to synthesize phloretin. The method mainly includes: exogenously expressing 4 coumarate Coenzyme A Ligase (4CL) and Chalcone synthase (CHS) which are two key enzymes for phloretin synthesis in the blue algae so as to use p-hydroxybenzene propanoic acid (also called phloretic acid)as the substrate to catalytically synthesize the phloretin, the 4CL catalyzes single-molecule p-hydroxybenzene propanoic acid to generate p-hydroxybenzene propionyl coenzyme A, the CHS catalyzes three-molecule malonyl coA and the single-molecule p-hydroxybenzene propionyl coenzyme A to synthesize the single-molecule phloretin. The method has the advantages that the phloretin is produced by usingone microorganism which can perform photosynthesis, the phloretin biological synthesizing approach which is cheap in raw material, simple in equipment, low in environment pollution and high in yield is created, and the method conforms to the direction of green production.

The invention relates to stabilized fertilizer, in particular to a nitrification inhibitor and application thereof in the stabilized fertilizer. The nitrification inhibitor is methyl p-hydroxyphenylpropionate; the stabilized fertilizer is prepared by adding the nitrification inhibitor, namely, the MHPP (Methyl p-hydroxyphenylpropionate), with the pure nitrogen content of 5 to 10 percent into ammonium nitrogen fertilizer or ammonium producing nitrogen fertilizer. The MHPP serving as the nitrification inhibitor has the advantages that the inhibiting effect is good, leaching loss of the nitrification inhibitor along with water is difficult to cause, the toxicity is low, the pollution to the environment is low (the nitrification inhibitor is prepared by extracting a plant source), and the like. After nitrogen fertilizer which contains the compound is applied to a farmland, a nitrifying process of the ammonium nitrogen fertilizer in soil can be effectively regulated and controlled, so that higher content of ammonium ions can be maintained in the soil for a long time, and the generation and accumulation of nitrate in the soil are reduced; therefore, the leaching loss of nitrogen element in the soil and the denitrification gaseous nitrogen loss are reduced; the fertilizer efficiency of the nitrogen fertilizer is prolonged; the absorption of crops to the nitrogen fertilizer is improved; the utilization rate of the nitrogen fertilizer is prolonged. The fertilizer can be applied as base fertilizer at one time; topdressing is not required; the purposes of saving labour and saving fertilizer are achieved.

The invention relates to a preparation method of a novel glycoside ceramide analogue with immunoregulation activity and an application thereof, and belongs to the technical field of chemistry and medicine. Based on the structural analysis of a receptor and combined with molecular docking and molecular dynamics simulation technologies, a category of compound with modified sugar ring (tryptophan, glutamic acid derivative or p-hydroxyphenylpropionic acid are introduced into R1 or R2 sites respectively) is designed and synthesized. Biological activity research shows that such compound has Th2 selectivity and has potential for use in pharmaceuticals. The novel compound has the following main synthetic characteristics: R1 is a hydroxyl group or a tryptophan derivative linked through an amide condensation reaction; R2 is a hydroxyl group or an L-glutamic acid derivative linked through amide condensation reaction, or p-hydroxybenzoic acid; R3 is -OBn, R4 is -N3;or R3 is -N3, R4 is -OBn;R5 maybe a substituent such as SEt, S-iPr, SPh, STOL, OC (NH) CCl3, and the like.

The invention relates to a preparation process of dihydrooat alkaloid D. The preparation process comprises the following three steps: phenolic hydroxyl group protection, acylating chlorination and amidation, and hydrolysis deprotection, wherein p-hydroxyphenylpropionic acid and acetic anhydride are used as raw materials to obtain p-acetoxyphenylpropionic acid; 2-[(p-acetoxy) benzene propionylamino] methyl benzoate is obtained by taking a chlorinated solvent as a reaction medium, taking a slightly excessive amount of thionyl chloride as an acylation reagent and taking magnesium oxide or calcium oxide as an acid-binding agent; hydrolyzing is carried out by adopting a sodium hydroxide solution, and acidifying is carried out by adopting a hydrogen chloride alcoholic solution to obtain the dihydrooat alkaloid D. According to the process, phenolic hydroxyl groups are protected, self-polymerization of a substrate is avoided, and generation of by-products is reduced; alkaline-earth metal oxide is selected as an acid-binding agent, material overflow is avoided, a hydrogen chloride alcoholic solution is used for replacing hydrochloric acid for acidification, a large amount of waste acid and waste water are avoided, toxic and harmful solvents are not used, and the method is economical and environmentally friendly. The process is easy to control, the yield is high, byproducts are few, and the method is suitable for industrial production of the dihydrooat alkaloid D.

The invention discloses a method for determining urease based on nano copper oxide. The method comprises the following steps: simulating enzymatic activity of alkaline peroxide based on the nano copper oxide, selectively hydrolyzing urea to generate ammonia and carbon dioxide in the existence of the urease, inhibiting the process in which the nano cooper oxide catalyzes the oxidation of hydrogen peroxide for p-hydroxyphenylpropionic acid by virtue of the ammonia, so that the generation of dipolymer fluorescent products is reduced, and a fluorescent analysis method adopting the nano copper oxide as a catalyst to determine the urease is provided. Urea, urease and a phosphate buffer are mixed to perform urease enzymatic reaction in a warm bath, then the p-hydroxyphenylpropionic acid, the hydrogen peroxide, the nano copper oxide and the phosphate buffer are added to continuously perform fluorescent reaction in the warm bath, a maximum excitation wavelength and a maximum emission wavelength of a fluorescent reaction product are respectively 320 nm and 409 nm. A linear range for determining the urease is 0.003 to 0.04 U/mL, and a detection limit is 2.6 U/L. The method can be used for determining the urease in soil.

The invention provides a brand-new phloretin derivative preparation method. The method comprises the steps of carrying out modified grafting on hydroxyl in p-hydroxyphenylpropionic acid in a phloretinsynthesis raw material in advance, and then carrying out a synthesis reaction with phloroglucinol to obtain a corresponding phloretin derivative, namely tetraacetylglucoside modified phloretin. The derivative not only retains the oxidation resistance and inflammation resistance of phloretin, but also has good water solubility and skin absorption efficiency, so that the obtained phloretin derivative can be widely applied to various forms of cosmetic products and has excellent oxidation resistance and inflammation resistance.

The invention discloses a synthesis process of methyl-3-(3-tert-butyl-4-hydroxy) phenylpropionate, which comprises the following steps of mixing methyl p-hydroxyphenylpropionate and tert-butyl alcoholaccording to a certain molar ratio, and carrying out continuous reaction in a tubular fixed bed reactor filled with a solid catalyst to obtain a crude product of methyl 3-(3-tert-butyl-4-hydroxy) phenylpropionate, and distilling to remove water with a low boiling point, carrying out secondary separation on residual tert-butyl alcohol through a rectifying tower to obtain a methyl 3-(3-tert-butyl-4-hydroxy) phenylpropionate product. According to the synthesis process, methyl p-hydroxyphenylpropionate and tert-butyl alcohol are used as raw materials, granular solid sulfonic acid resin is used, continuous reaction is performed, secondary separation is performed through a rectifying tower, a high-purity methyl 3-(3-tert-butyl-4-hydroxy) phenylpropionate product is obtained, the yield is high,the quality is excellent, unreacted methyl p-hydroxyphenyl propionate is recycled, so that the method has good industrial production value, and the production cost is reduced.

The invention discloses a preparation method of dihydrooat alkaloid, and belongs to the technical field of organic synthesis. Hydroxyl is protected by p-hydroxyphenylpropionic acid and vinyl alkyl ether under the action of a catalyst, then the p-hydroxyphenylpropionic acid and anthranilate are condensed under the action of the catalyst to generate acid amine, and then the dihydrooat alkaloid is obtained through inorganic alkali/acid deprotection or acid deprotection. The method is completed in three steps, the product is easy to separate, the total yield of the three steps is up to 80-85%, the reaction is coherent, the used reagent is friendly to equipment, and the product purity can reach 99.8%.

The invention discloses a preparation method of dihydro-oat alkaloid, which comprises the following steps: S1, preparation of p-hydroxyphenyl propionate, S2, preparation of a dihydro-oat alkaloid crude product, and S3, preparation of a dihydro-oat alkaloid competitive product.The amine ester exchange catalyst is preferably concentrated sulfuric acid and p-toluenesulfonic acid, so that the reaction is more facilitated; although the raw material has an aniline structure and can form aniline salt at low temperature, when the temperature is higher than 120 DEG C, the unstable aniline salt can be dissociated and continuously participates in the reaction, so that the conversion rate of the raw material is increased; the amine ester exchange solvent is preferably DMSO (dimethylsulfoxide) and sulfolane, the two solvents have the properties of high boiling point and good solubility, and meanwhile, the solvents are low in toxicity, have water solubility, are simple in post-treatment, and are convenient for refining and purifying a dihydrooat alkaloid crude product; meanwhile, the preparation process is short in step, the yield of the two-step reaction is 80% or above, and industrial large-scale production is facilitated.

The invention discloses a preparation method of a spraying hydrogel for treating solar dermatitis. The preparation method comprises the following steps of: preparing a gelatin-p-hydroxyphenylpropionic acid copolymer, a sprayable hydrogel liquid A and a sprayable hydrogel liquid B, and finally preparing the sprayable hydrogel. The spraying hydrogel prepared by the invention can realize ultraviolet radiation resistance, moisture retention, inflammation resistance and allergy resistance of the skin of an affected part, also can realize slow release and continuous transdermal absorption of an anti-inflammatory drug dexamethasone sodium phosphate, and can realize the effects of on-demand administration and timely drug stop for solar dermatitis. The coating of the spraying hydrogel can be well attached to the irregular skin surface of any patient, the thickness of the coating is controllable, toughness is high, peeling and cracking are avoided, the body feeling is comfortable, and the double effects of sunscreen and treatment on the skin of the patient can be effectively achieved.

The invention discloses a method for increasing stability of bioprosthetic heart valve glycosaminoglycan. The method comprises the following processes: Modification of pig or bovine pericardium with p-hydroxyphenylpropionic acid or p-hydroxyphenylethylamine, modification of neomycin sulfate with p-hydroxyphenylpropionic acid, and initiation of enzyme cross-linking under conditions of horseradish peroxidase and hydrogen peroxide. A phenolic hydroxyl group can be introduced into the p-hydroxyphenylpropionic acid modified neomycin sulfate, and horseradish peroxidase and hydrogen peroxide will achieve chemical crosslinking of the phenolic hydroxyl group. The method provided by the invention can raise the glycosaminoglycan stability and anti-calcification performance of bioprosthetic heart valve and potentially prolong its service life.

The invention discloses a process for synthesizing methyl p-hydroxyphenylpropionate. The process comprises the following steps of: catalyzing a 3-(3, 5-di-tert-butyl-4-hydroxy) methyl phenylpropionateraw material by using an acid catalyst, and carrying out continuous reaction rectification in a reaction rectification kettle to obtain the methyl p-hydroxyphenylpropionate. According to the technological process, raw materials are fully utilized, the catalyst dosage is small, the production technology is simple, and operation is convenient; meanwhile, the generated by-product isobutene is recycled and can be used as a German raw material of other products, so that no three wastes are generated in the whole technological process, and the process is an environment-friendly process; the methylp-hydroxyphenylpropionate prepared by the synthesis process is relatively high in yield and excellent in quality, and has a very good industrial production value.