Method of synthesizing general 2-methoxy phosphoric acid ester pesticide hapten

Abstract

The invention relates to a synthetic method of dimethoxy phosphate ester type pesticide universal hapten, and belongs to the technical field of the biochemical engineering. The synthetic process of the invention comprises three steps of esterification, acylation and hydrolysis. Firstly, para hydroxybenzene propionic acid and carbinol have esterification reaction to make carboxy metho esterified; and then the obtained para hydroxybenzene methyl propionate has reaction with dimethoxy phosphorus oxychloride to obtain dimethoxy phosphate ester; finally the methyl ester formed in the first step is hydrolyzed again, and carboxyl is reduced to obtain O, O-dimethyl-O-(4-propionyloxy phenyl group) phosphate ester, and the phosphate ester is coupled with egg albumin to be the envelope antigen separated out by redundant ELISA of the dimethoxy phosphate ester type pesticide. The substance is used as the envelope antigen during the process of enzyme linking immunity checking of the pesticide after being coupled with the albumen, and the structure has benzene ring, thereby using the difference of envelope antigen and the immunity primary structure to further improve the checking sensitivity and reduce the checking limitation.

Description

technical field [0001] The invention discloses a method for synthesizing a common hapten of dimethoxyphosphate pesticides, which belongs to the technical field of biochemical engineering. Background technique [0002] The basic chemical structure of organophosphorus pesticides is: [0003] [0004] Dimethoxy phosphate is a structure in which R and R / are both methyl and Y is oxygen. A general hapten O is synthesized for a class of pesticides with this structure, O-dimethyl-O-(4 - propionyl phenyl) phosphate. Organophosphorus pesticides have been widely used in my country's agricultural production practice due to their broad insecticidal spectrum and high efficacy. Due to their large usage and wide range of applications, organophosphorus pesticides have received widespread attention in environmental protection and food safety. The development of immunoassay methods in the detection of pesticide residues is very rapid. At present, more than a dozen commonly used organopho...

AI Technical Summary

Problems solved by technology

For the multi-residue ELISA research of organophosphorus pesticides, some attempts have been made at home and abroad, but so far, for several highly toxic phosphate pesticides such as monocrotophos, phosphamide, memephos, dicrotophos, dichlorvos, etc. There is no report on its multi-residue analysis

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