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Method of synthesizing general 2-methoxy phosphoric acid ester pesticide hapten

A technology of dimethoxyphosphoric acid and synthesis method, which is applied in chemical instruments and methods, compounds of Group 5/15 elements of the periodic table, organic chemistry, etc., to achieve the effect of reducing the detection limit and improving the detection sensitivity

Inactive Publication Date: 2009-09-09
福建漳州鼎能生物科技有限公司
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Problems solved by technology

For the multi-residue ELISA research of organophosphorus pesticides, some attempts have been made at home and abroad, but so far, for several highly toxic phosphate pesticides such as monocrotophos, phosphamide, memephos, dicrotophos, dichlorvos, etc. There is no report on its multi-residue analysis

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  • Method of synthesizing general 2-methoxy phosphoric acid ester pesticide hapten
  • Method of synthesizing general 2-methoxy phosphoric acid ester pesticide hapten
  • Method of synthesizing general 2-methoxy phosphoric acid ester pesticide hapten

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Embodiment Construction

[0028] (1) Esterification

[0029] Add 3.5g (21mmol) of p-hydroxyphenylpropionic acid, 14mL of methanol and 1mL of concentrated sulfuric acid into a 50mL flask, and heat to reflux at 120°C for 2h. Afterwards, about 5mL of methanol was distilled off, and a saturated solution of sodium carbonate was added until no gas was generated. Then it was extracted with dichloromethane, the solvent was removed by rotary evaporation at 35°C, and the reddish-brown oil was obtained by vacuum drying. The purity of the product was over 99%.

[0030] (2) Acylation

[0031] In a 50mL round bottom flask, add 10-15mL of acetonitrile as a solvent, add 1.9280g (10mmoL) of the first product methyl p-hydroxyphenylpropionate, 10mmoL of dimethoxyphosphoryl chloride, and 2.5g of potassium carbonate, and stir magnetically at room temperature For about 2 hours, the progress of the reaction was continuously monitored by TLC during the reaction until the acid chloride was completely reacted. Then let it st...

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Abstract

The invention discloses a method for synthesizing a common hapten of dimethoxyphosphate pesticides, which belongs to the technical field of biochemical engineering. The synthesis process of the present invention is divided into three steps of esterification, acylation and hydrolysis. First, p-hydroxyphenylpropionic acid is esterified with methanol to esterify carboxyl methyl; Methoxyphosphoryl chloride reaction to obtain dimethoxyphosphate; finally, the methyl ester formed in the first step of esterification is hydrolyzed again, and the carboxyl group is reduced to obtain O, O-dimethyl-O-(4-propionic acid phenyl) phosphate; this phosphate is coupled with ovalbumin as a coating antigen for multi-residue ELISA analysis of dimethoxyphosphate pesticides. After the substance is coupled with the protein, it is used as the coating antigen in the process of this type of pesticide ELISA, because the structure contains a benzene ring, and the difference in the structure of the coating antigen and the immunogen is used to further improve the detection sensitivity and reduce the detection limit. .

Description

technical field [0001] The invention discloses a method for synthesizing a common hapten of dimethoxyphosphate pesticides, which belongs to the technical field of biochemical engineering. Background technique [0002] The basic chemical structure of organophosphorus pesticides is: [0003] [0004] Dimethoxy phosphate is a structure in which both R and R' are methyl and Y is oxygen. A general hapten O is synthesized for a class of pesticides with this structure, O-dimethyl-O-(4 - propionyl phenyl) phosphate. Organophosphorus pesticides have been widely used in my country's agricultural production practice due to their broad insecticidal spectrum and high efficacy. Due to their large usage and wide range of applications, organophosphorus pesticides have received widespread attention in environmental protection and food safety. The development of immunoassay methods in the detection of pesticide residues is very rapid. At present, more than a dozen commonly used organopho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/12
Inventor 胥传来尹丽梅陶冠军秦昉李雅丽陈伟彭池方袁媛
Owner 福建漳州鼎能生物科技有限公司
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