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Method for preparing lactose-N-disaccharide

A technology of lactose and galactose phosphate, applied in the field of preparation of lactose-N-disaccharide, can solve the problems of no benefit to human health, the activity and effect cannot be compared with that of breast milk oligosaccharide, and the function of breast milk oligosaccharide cannot be simulated, and the cost is achieved. Low, high utility value effect

Inactive Publication Date: 2017-04-26
李拖平
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Problems solved by technology

Although some oligosaccharides on the market are added to milk as prebiotics to increase the number of bifidobacteria in the intestinal tract of infants, their activity and effect cannot be compared with breast milk oligosaccharides due to the limitation of their chemical structure. Mimics all functions of breast milk oligosaccharides
Moreover, these artificially synthesized oligosaccharides have no selectivity for the proliferation of intestinal microorganisms. In addition to bifidobacteria, they can also proliferate a variety of other intestinal bacteria, and some bacteria are not intestinal probiotics and have no effect on human health. benefit

Method used

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  • Method for preparing lactose-N-disaccharide

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preparation example Construction

[0023] A kind of preparation method of lactose-N-disaccharide

[0024] 1 μM-2M 1-galactose phosphate, 1 μM-2M N-acetylglucosamine, 1 μM-2M phosphate buffer (pH 3-9), 0.01-10000U lactose-N-disaccharide phosphorylase in 5- After reacting at 50°C for 2 minutes to 1 month, lactose-N-disaccharide (Gal-β1,3-GlcNAc) can be produced, and the structural formula is (I). The pure lactose-N-disaccharide product can be obtained after the reaction solution is concentrated, refined and crystallized. The molecular weight of the product is 383 as detected by mass spectrometry, and its structure is confirmed as lactose-N-disaccharide by nuclear magnetic resonance detection. The yield of lactose-N-disaccharide can reach 87%.

[0025]

Embodiment 1

[0026] Embodiment 1 A kind of preparation method of lactose-N-disaccharide

[0027] Phosphorylate 10mM sucrose, 10mM uridine diphosphate glucose, 50U sucrose phosphorylase, UDP-glucose hexose phosphate uridyltransferase, UDP-glucose epimerase, 100U lactose-N-disaccharide Enzyme, 10mM N-acetylglucosamine and 100mM phosphate buffer (pH 6-7) are placed in the same reaction system, and after the reaction system is reacted at 30°C for 10 days, lactose-N-disaccharide can be generated. The pure lactose-N-disaccharide product can be obtained after the reaction solution is concentrated, refined and crystallized. The molecular weight of the product is 383 as detected by mass spectrometry, and its structure is confirmed as lactose-N-disaccharide by nuclear magnetic resonance detection. The yield of lactose-N-disaccharide reached 80%.

Embodiment 2

[0028] Embodiment 2 A kind of preparation method of lactose-N-disaccharide

[0029] 1M sucrose, 1M uridine diphosphate galactose, 10000U immobilized sucrose phosphorylase, UDP-glucose epimerase, UDP-glucose hexose uridine phosphate immobilized on the sodium alginate carrier Acyltransferase, 10000U lactose-N-disaccharide phosphorylase, 2M N-acetylglucosamine and 1M phosphate buffer (pH 8-9) were placed in the same reaction system, and the reaction system was reacted at 50°C After 20 minutes, lactose-N-disaccharide can be produced. The pure lactose-N-disaccharide product can be obtained after the reaction solution is concentrated, refined and crystallized. The molecular weight of the product is 383 as detected by mass spectrometry, and its structure is confirmed as lactose-N-disaccharide by nuclear magnetic resonance detection. The yield of lactose-N-disaccharide was about 3%.

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Abstract

The invention discloses a method for preparing lactose-N-disaccharide. 1-phosphate galactose, N-acetyl glucosamine, lactose-N-disaccharide phosphorylase and phosphate buffer are placed in the same reaction system and mixed to synthesize the objective product lactose-N-disaccharide. The 1-phosphate galactose is prepared by using starch, sucrose, dextrin and other cheap raw materials. The simple method is used for one-step synthesis of the objective product lactose-N-disaccharide, the preparation cost of the product is low, and the practical value is high.

Description

technical field [0001] The invention relates to the field of oligosaccharide synthesis, in particular to a preparation method of lactose-N-disaccharide. Background technique [0002] A type of breast milk oligosaccharide contained in breast milk is a special oligosaccharide that is not contained in the milk of other mammals in nature. Recent studies have shown that it is a specific food source for bifidobacteria and is not restricted by other bacteria. use. In exclusively breastfed infants, bifidobacteria account for more than 90% of the total intestinal bacteria, even as high as 99%. Although some oligosaccharides on the market are added to milk as prebiotics to increase the number of bifidobacteria in the intestinal tract of infants, their activity and effect cannot be compared with breast milk oligosaccharides due to the limitation of their chemical structure. Simulate all the functions of breast milk oligosaccharides. Moreover, these artificially synthesized oligosacc...

Claims

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Application Information

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IPC IPC(8): C12P19/26C12P19/24
CPCC12P19/24C12P19/26
Inventor 李拖平孙晓李苏红
Owner 李拖平
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