Method for preparing lactose-N-disaccharide
A technology of lactose and galactose phosphate, applied in the field of preparation of lactose-N-disaccharide, can solve the problems of no benefit to human health, the activity and effect cannot be compared with that of breast milk oligosaccharide, and the function of breast milk oligosaccharide cannot be simulated, and the cost is achieved. Low, high utility value effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0023] A kind of preparation method of lactose-N-disaccharide
[0024] Put 1μM-2M galactose-1-phosphate, 1μM-2M N-acetylglucosamine, 1μM-2M phosphate buffer (pH 3-9), 0.01-10000U lactose-N-disaccharide phosphorylase in 5- After reacting at 50℃ for 2min-1 month, lactose-N-disaccharide (Gal-β1,3-GlcNAc) can be formed, and the structural formula is (I). The pure lactose-N-disaccharide product can be obtained after the reaction liquid is concentrated, refined and crystallized. The molecular weight of the product was 383 by mass spectrometry, and its structure was confirmed to be lactose-N-disaccharide by nuclear magnetic resonance. Its lactose-N-disaccharide yield can reach 87%.
[0025]
Example Embodiment
[0026] Example 1 A preparation method of lactose-N-disaccharide
[0027] Phosphorylate 10mM sucrose, 10mM uridine diphosphate glucose, 50U sucrose phosphorylase, UDP-glucose hexose phosphate uridylyltransferase, UDP-glucose epimerase, 100U lactose-N-disaccharide Enzyme, 10mM N-acetylglucosamine and 100mM phosphate buffer (pH 6-7) are placed in the same reaction system. After the reaction system is reacted at 30°C for 10 days, lactose-N-disaccharide can be produced. The pure lactose-N-disaccharide product can be obtained after the reaction liquid is concentrated, refined and crystallized. The molecular weight of the product was 383 by mass spectrometry, and its structure was confirmed to be lactose-N-disaccharide by nuclear magnetic resonance. The yield of lactose-N-disaccharide reaches 80%.
Example Embodiment
[0028] Example 2 A preparation method of lactose-N-disaccharide
[0029] 1M sucrose, 1M uridine diphosphate galactose diphosphate, 10000U immobilized sucrose phosphorylase, UDP-glucose epimerase, UDP-glucose hexose uridine phosphate immobilized on a sodium alginate carrier Acyltransferase, 10000U lactose-N-disaccharide phosphorylase, 2M N-acetylglucosamine and 1M phosphate buffer (pH 8-9) are placed in the same reaction system, and the reaction system will react at 50℃. After 20 minutes, lactose-N-disaccharide can be produced. The pure lactose-N-disaccharide product can be obtained after the reaction liquid is concentrated, refined and crystallized. The molecular weight of the product was 383 by mass spectrometry, and its structure was confirmed to be lactose-N-disaccharide by nuclear magnetic resonance. The yield of lactose-N-disaccharide is about 3%.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap