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Precious metal plasma effect enhanced up-conversion fluorescence structure-based method for hypersensitive detection of vascular endothelial growth factor

A technology of vascular endothelium and growth factors, applied in the field of nanomaterials and analytical chemistry, can solve the problems of high monoclonal antibody consumption, high consumption, heavy monoclonal antibody screening, etc.

Inactive Publication Date: 2017-04-26
JIANGNAN UNIV
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Problems solved by technology

[0003] Traditional VEGF detection methods, such as ELISA, have high requirements for monoclonal antibodies, and the screening of monoclonal antibodies is a heavy and costly task

Method used

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  • Precious metal plasma effect enhanced up-conversion fluorescence structure-based method for hypersensitive detection of vascular endothelial growth factor
  • Precious metal plasma effect enhanced up-conversion fluorescence structure-based method for hypersensitive detection of vascular endothelial growth factor
  • Precious metal plasma effect enhanced up-conversion fluorescence structure-based method for hypersensitive detection of vascular endothelial growth factor

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Embodiment 1

[0046] (1) Synthesis of up-conversion nanoparticles (27.1nm): NaYF prepared by high-temperature pyrolysis 4: Yb, Tm upconverting nanoparticles. Weigh YCl 3 ·6H 2 O, YbCl 3 ·6H 2 O, TmCl 3 ·6H 2 O(Ln: 79.5mol% Y 3+ , 20mol% Yb 3+ , 0.5mol%Tm 3+ , a total of 1mmol) in a 100ml three-necked flask, add 4mL oleic acid and 16mL 1-octadecene. Stir slowly and gradually raise the temperature to 160°C, keep for 30min, and cool naturally to room temperature. Accurately weigh 0.1g of sodium hydroxide and 0.148g of ammonium fluoride, dissolve them in 10mL of methanol solution and mix well. The above solution was slowly added into a three-necked flask, the temperature was raised to 50° C., and magnetically stirred for 30 minutes. Raise the temperature to 80°C and keep for 30min to slowly evaporate the methanol. After degassing, the mixture is heated to 300°C and reacted for 1h. After the reaction, it was naturally cooled to room temperature, washed three times with cyclohexane and...

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Abstract

A precious metal plasma effect enhanced up-conversion fluorescence structure-based method for the hypersensitive detection of a vascular endothelial growth factor relates to the fields of nano-materials and analytical chemistry. A standard curve between the intensity of a fluorescence signal and the concentration of the vascular endothelial growth factor is established through synthesizing up-conversion nano-particles (NaYF4, Yb, Tm) with the particle size of 27.1 nm, synthesizing gold nano-particles with the particle size of 4.6 nm, modifying the up-conversion nano-particles, wrapping the gold nano-particles, carrying out single-strand DNA modification on the up-conversion nano-particles, carrying out single-strand DNA modification on the surface of the gold nano-particles, serially regulating a spacing between the particles, and carrying out enhanced regulation and intensity detection on the up-conversion fluorescence signal. Compared with traditional detection methods, the fluorescence enhanced signal-based method for detecting the vascular endothelial growth factor has the advantages of low background, high sensitivity, convenience, fastness and very good practical application prospect.

Description

technical field [0001] A method for ultrasensitive detection of vascular endothelial growth factor based on noble metal plasma effect-enhanced upconversion fluorescent structure, involving the fields of nanomaterials and analytical chemistry. Background technique [0002] Vascular endothelial growth factor (VEGF) is a kind of glycoprotein acting on vascular endothelial cells, which has a strong effect of promoting endothelial cell proliferation and promoting the formation of new blood vessels. The growth of tumor tissue must rely on angiogenesis to provide sufficient nutrients and oxygen to maintain. Therefore, VEGF has been considered as a tumor marker. The detection of tumor markers helps to detect cancer early and achieve the purpose of early treatment. [0003] Traditional VEGF detection methods, such as ELISA, have high requirements for monoclonal antibodies, and the screening of monoclonal antibodies is a heavy and costly task. The invention proposes a novel, conveni...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N21/64
CPCG01N21/6428G01N33/57496G01N33/6872G01N2333/485
Inventor 王周平赵森吴世嘉段诺马小媛夏雨
Owner JIANGNAN UNIV
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