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Method for building candida albicans infected chicken models

A technology of Candida albicans and model building, which is applied in the field of medicine and biology, can solve problems such as loss, and achieve the effect of stable infection, high infection rate, and easy control of infection conditions

Inactive Publication Date: 2017-05-10
HEBEI UNIV OF ENG
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  • Application Information

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Problems solved by technology

It was previously believed that poultry candidiasis is often sporadic, and once it breaks out, it can cause huge losses

Method used

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  • Method for building candida albicans infected chicken models
  • Method for building candida albicans infected chicken models
  • Method for building candida albicans infected chicken models

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Experimental program
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Embodiment Construction

[0017] Verification experiment: 1. Experimental strain: Candida albicans HE strain is a clinical isolate. On the potato agar plate, the colony is round, milky white, and has no pigmentation on the back. Under the microscope, the bacteria are of different sizes and are round or oval yeast-like bacteria. The strain was identified and confirmed by biochemical tests and 16sRNA gene sequencing.

[0018] 2. Experimental animals: 16 healthy 7-day-old chicks, provided by the experimental animal breeding farm of Hebei Engineering University, with an average weight of 30±4g, half male and half male.

[0019] 3. Grouping of experimental animals and treatment of strains: randomly divided into 2 groups according to body weight and sex, with 8 animals in each group. Group A was a blank control group, which was treated with glucocorticoids but not inoculated with Candida albicans; Corticosteroid treatment, while inoculation of Candida albicans HE strain;

[0020] 4. Preparation of conidia ...

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Abstract

The invention provides a method for building candida albicans infected chicken models. The method includes steps of a, preparing candida albicans strain conidium suspension, to be more specific, inoculating clinically isolated candida albicans strains on potato agar culture media, culturing the candida albicans strains at the temperature of 25 DEG C for 2.5-4 days, washing candida albicans colonies by the aid of PBS (phosphate buffer solution), collecting washing solution with candida albicans conidia or hyphae, centrifugally discarding supernatant, collecting precipitates, grinding the precipitates and diluting the precipitates until the concentration of the candida albicans conidia reaches 1*10<6> / mL so as to obtain the conidium suspension for standby application; b, forming the models, to be more specific, acquiring just hatched chicks, injecting 1mL of conidium suspension into the crop of each chick when the chick is 7 days old, raising the vaccination chicks in coops and allowing the chicks to freely drink water and feed for 4-9 days so as to successfully build the models. The method has the advantages that the models are made from chicken infected by clinically isolated candida albicans (HE strains), and accordingly discussion on candida albicans infection of the chicken is close to reality and is accurate.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a method for establishing a chicken model infected by Candida albicans. Background technique [0002] Candidiasis is a fungal disease caused by Candida spp., mainly Candida albicans infection, and is a zoonotic disease. It can invade human mucous membranes, skin, tissues, organs, etc. and cause different degrees of candidiasis albicans. With the wide application of broad-spectrum antibiotics, corticosteroids, anti-tumor chemotherapy and radiotherapy, and interventional diagnosis and treatment methods, as well as the reduction of body resistance due to viral infections, wasting diseases, etc., the infections caused by Candida albicans have gradually increased. The emergence of drug strains makes clinical treatment more difficult. [0003] Under natural conditions, many kinds of poultry are susceptible, mainly harming young poultry. The pathogen has long...

Claims

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Application Information

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IPC IPC(8): C12N1/16A01K67/027C12R1/725
CPCA01K67/027A01K2207/20A01K2227/30A01K2267/0337C12N1/16
Inventor 刘彦威刘娜刘建钗刘利强沈洁张欣史瑞雅李博
Owner HEBEI UNIV OF ENG
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