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Preparation method of nanogold copper quenching zinc-cadmium sulfide electrofluorescence insulin sensor

A zinc-cadmium sulfide, nano-gold technology, applied in chemiluminescence/bioluminescence, instruments, scientific instruments, etc., can solve the problems of cumbersome operation and low sensitivity, and achieve simple operation, good electroluminescence performance, and wide signal response range. Effect

Active Publication Date: 2017-05-10
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the analysis methods for detecting insulin mainly include immunoassay and chromatography, but these two methods are cumbersome to operate and have low sensitivity. Therefore, in order to overcome the shortcomings of the above traditional analysis methods, the present invention designs a method with strong specificity and high sensitivity. Fast and easy-to-operate electroluminescence immunoassay

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation method of nano-gold copper quenched zinc cadmium sulfide electroluminescent insulin sensor

[0043] (1) Polish the glassy carbon electrode with 1.0, 0.3, 0.05 μm alumina powder in sequence, ultrasonically clean it in ultrapure water and ethanol, and dry it with nitrogen;

[0044] (2) Add 6 μL of gold-coated zinc cadmium sulfide / aminated nitrogen-doped mesoporous carbon solution Au@Zn with a concentration of 0.5 mg / mL x Cd 1-x S / NMC is drip-coated onto the electrode surface and dried;

[0045] (3) Add 6 µL of insulin capture antibody Ab with a concentration of 5 µg / mL dropwise 1 solution, incubated in a refrigerator at 4°C for 1 h, washed with ultrapure water, and dried in the air;

[0046] (4) Continue to drop 3 μL of 1% bovine serum albumin solution BSA, incubate in a refrigerator at 4 °C for 1 h, wash with ultrapure water, and dry in the air;

[0047] (4) Incubate 6 μL of insulin antigen Ag with a series of different concentrations ranging fr...

Embodiment 2

[0049] Example 2 Preparation method of nano-gold copper quenched zinc cadmium sulfide electroluminescent insulin sensor

[0050] (1) Polish the glassy carbon electrode with 1.0, 0.3, 0.05 μm alumina powder in sequence, ultrasonically clean it in ultrapure water and ethanol, and dry it with nitrogen;

[0051] (2) Add 6 μL of gold-coated zinc cadmium sulfide / aminated nitrogen-doped mesoporous carbon solution Au@Zn with a concentration of 3 mg / mL x Cd 1- x S / NMC is drip-coated onto the electrode surface and dried;

[0052] (3) Add 6 µL of insulin capture antibody Ab with a concentration of 10 µg / mL dropwise 1 solution, incubated in a refrigerator at 4°C for 1 h, washed with ultrapure water, and dried in the air;

[0053] (4) Continue to drop 3 μL of 2% bovine serum albumin solution BSA, incubate in a refrigerator at 4 °C for 1 h, wash with ultrapure water, and dry in the air;

[0054] (4) Incubate 6 μL of insulin antigen Ag with a series of different concentrations ranging f...

Embodiment 3

[0056] Example 3 Preparation method of nano-gold copper quenched zinc cadmium sulfide electroluminescent insulin sensor

[0057] (1) Polish the glassy carbon electrode with 1.0, 0.3, 0.05 μm alumina powder in sequence, ultrasonically clean it in ultrapure water and ethanol, and dry it with nitrogen;

[0058] (2) Add 6 μL of gold-coated zinc cadmium sulfide / aminated nitrogen-doped mesoporous carbon solution Au@Zn with a concentration of 5 mg / mL x Cd 1- x S / NMC is drip-coated onto the electrode surface and dried;

[0059] (3) Add 6 µL of insulin capture antibody Ab with a concentration of 15 µg / mL dropwise 1 solution, incubated in a refrigerator at 4°C for 1 h, washed with ultrapure water, and dried in the air;

[0060] (4) Continue to drop 3 μL of 3% bovine serum albumin solution BSA, incubate in a refrigerator at 4 °C for 1 h, wash with ultrapure water, and dry in the air;

[0061] (4) Incubate 6 μL of insulin antigen Ag with a series of different concentrations ranging f...

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Abstract

The invention relates to a preparation method of a nanogold copper quenching zinc-cadmium sulfide electrofluorescence insulin sensor, and belongs to the field of an electrofluorescence sensor. The electroluminescence spectrum of zinc-cadmium sulfide is well overlapped with the ultraviolet absorption atlas of nanogold copper; the nanogold copper can be used as a receptor to generate energy resonance transfer with the zinc-cadmium sulfide, so that the electrofluorescence response of the zinc-cadmium sulfide is quenched. Gold coats the zinc-cadmium sulfide / amino nitrogen doping mesoporous carbon Au@ZnxCd1-xS / NMC to be used as an insulin capture antibody substrate material. The nanogold copper AuCu is used as an insulin detection antibody immobilization material. Through zinc doping, the CdS electrofluorescence response can be improved; ZnxCd1-xS is used as an electrofluorescence signal source; the quenching effect of AuCu on Au@ZnxCd1-xS is used; the detection on insulin is realized according to different electrochemical luminous signal intensities of the insulin with different concentrations.

Description

technical field [0001] The invention relates to a preparation method of a nanometer gold copper quenching zinc cadmium sulfide electroluminescent insulin sensor. It specifically involves a zinc cadmium sulfide as a luminescent material, using the excellent conductivity and large specific surface area of ​​aminated nitrogen-doped mesoporous carbon as a substrate material to increase the immobilization capacity of luminous bodies and antibodies, and nano-gold copper as a secondary antibody marker A sandwich-type electrochemiluminescence immunosensor for detecting insulin is prepared, which belongs to the technical field of electrochemiluminescence detection. Background technique [0002] Insulin is a protein hormone secreted by pancreatic islet cells. It can not only promote the glucose in the blood to enter the liver, muscle and fat tissue cells, but also promote the oxidation and decomposition of glucose to release energy for the body to use. It is the main hormone to mainta...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/327G01N33/532
CPCG01N21/76G01N27/327G01N33/532
Inventor 朱文娟魏琴张勇马洪敏吴丹闫涛匡轩
Owner UNIV OF JINAN
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