Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Reagent kit for detecting ochratoxin A in food

An ochratoxin and detection kit technology, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of unsuitability for popularization and popularization, insufficient sensitivity and high technical requirements, and achieves simple sample pretreatment, wide application and specificity. strong effect

Inactive Publication Date: 2017-05-10
BEOSON JIANGSU FOOD SAFETY TECH CO LTD
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, chromatography is not sensitive enough for the detection of trace fungi in food. Although the liquid-mass spectrometry method has high sensitivity and fast speed, the equipment is expensive, and only professional technicians should be required to perform the detection. The technical requirements are high, and it is not suitable for popularization and universal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] In the embodiment of the present invention, a detection kit for ochratoxin A in food, comprising: porous coated plate, buffer, ochratoxin A standard substance, anti-ochratoxin A antibody, europium-labeled rabbit anti-mouse antibody , washing liquid, and luminescent liquid; the luminescent liquid contains 2-2.4g / L polyethylene glycol 600, 0.16-0.18wt.% Tween-20, 0.9-1.2g / L 4-iodophenylboronic acid, 0.18-0.2g / L p-iodophenol, 22-24ml / L dimethylformamide, 12-14g / L polyvinylpyrrolidone, 3-5g / L ethylenediaminetetraacetic acid, 1-1.2 million units / L gentamicin sulfate, 0.5-0.55g / L urea hydrogen peroxide, 0.12-0.15mol / L tris buffer solution with a pH value of 9.2-9.4.

[0019] 1. Preparation of immunogen and coating agent

[0020] Synthesis of immunogen (ochratoxin A-bovine serum albumin): Accurately weigh 324 mg of ochratoxin A and dissolve in 2 mL of N,N-dimethylformamide, add γ-aminobutyric acid solution drop by drop under stirring , stirred and reacted for 3 hours, and a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a reagent kit for detecting ochratoxin A in food. The reagent kit comprises a porous coated plate, a buffer solution, an ochratoxin A standard substance, an ochratoxin A antibody, an europium-marked rabbit anti-mouse antibody, a washing solution and a luminous solution. The luminous solution compris, 2-2.4g / L of polyethylene glycol 600, 0.16-0.18wt% of polysorbate-20, 0.9-1.2g / L of 4-Iodophenylboronic acid, 0.18-0.2g / L of p-Iodophenol, 22-24ml / L of dimethylformamide, 12-14 g / L of polyvinylpyrrolidone, 3-5g / L of ethylenediamine tetraacetic acid, 1-1.2 million units / L of gentamicin sulphate, 0.5-0.55g / L of hydrogen peroxide urea and 0.12-0.15mol / L of tris (hydroxymethyl) aminomethane buffer solution with pH value ranging from 9.2-9.4. The reagent kit for detecting the ochratoxin A in food has the advantages of simplicity, delicacy, accuracy and quickness in operation and is applicable to quick detection for large batches of samples.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a detection kit for ochratoxin A in food. Background technique [0002] Ochratoxins are secondary metabolites produced by Aspergillus chraceors, Pmicilium vermculosutm, Penicilium viridicatum, and several other fungi of the genus Penicillium. Ochratoxin is a series of isocoumarin derivatives, including A, B, C and other 7 compounds with similar structures. Among them, ochratoxin A is the most toxic, can damage the liver and kidney of animals, and has teratogenic, carcinogenic and mutagenic effects, and is considered to be related to the occurrence of human Balkan nephropathy. The International Agency for Research on Cancer (IARC) has classified ochratoxin A as a Group 2B carcinogen. Ochratoxin A often contaminates crops such as wheat, corn, cereals, coffee and grapes. Therefore, establishing a sensitive and rapid detection method is an important technical prerequisite f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/543G01N33/533
CPCG01N33/533G01N33/543
Inventor 张进吴念绮周朱晨张根义胡彬朱倩倩
Owner BEOSON JIANGSU FOOD SAFETY TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com