Sturgeon compound powder, sturgeon bone wine and application
A technology of composite powder and sturgeon, which is applied in the field of sturgeon bone wine and its application, composite powder of sturgeon, to achieve the effects of improving the body's immunity, enriching nutrition and protecting the liver
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Embodiment 1
[0020] A sturgeon compound powder is prepared as follows:
[0021] a. Wash the slaughtered sturgeon, take the sturgeon meat and sturgeon cartilage and freeze-dry to a moisture content of less than 1%, crush the freeze-dried sturgeon meat and sturgeon cartilage into 2500 mesh sturgeon superfine powder;
[0022] b. Grind the ultrafine sturgeon powder into 100 nm nano sturgeon powder;
[0023] c. Add 0.05M phosphate buffer with pH 7~8 and compound enzyme to sturgeon nanopowder, and enzymatically hydrolyze for 30 h; the mass ratio of sturgeon nanopowder to phosphate buffer is 1:5, and the compound enzyme The added amount is 15 activity units: 1 gram of sturgeon nanopowder, and the compound enzyme is a mixture of subtilisin, papain, and alkaline protease in a ratio of activity units of 3:3:4;
[0024] d. Centrifuge the supernatant, use an ultrafiltration membrane separator with a molecular weight cut-off of less than 10000 Da to separate, collect the liquid passing through the ultrafiltrat...
Embodiment 2
[0029] a. Wash the slaughtered sturgeon, take the sturgeon meat and sturgeon cartilage and freeze-dry it to a moisture content of less than 1%, crush the freeze-dried sturgeon meat and sturgeon cartilage into 1000 mesh sturgeon superfine powder;
[0030] b. The ultrafine sturgeon powder ball is ground into 70nm sturgeon nanopowder;
[0031] c. Add the 0.1M phosphate buffer of pH 7~8 and the compound enzyme to the sturgeon nanopowder, and enzymatically hydrolyze for 12 hours; the mass ratio of the sturgeon nanopowder to the phosphate buffer is 1:1, and the compound enzyme is added The amount is 25 vitality units: 1 gram of sturgeon nanopowder, and the composite enzyme is a mixture of subtilisin, papain, and alkaline protease in a ratio of 3:3:4 to the vitality unit;
[0032] d. Centrifuge the supernatant, use an ultrafiltration membrane separator with a molecular weight cut-off of less than 10000 Da to separate, collect the liquid passing through the ultrafiltration membrane, pass the...
Embodiment 3
[0034] a. Wash the slaughtered sturgeon, take the sturgeon meat and sturgeon cartilage and freeze-dry to a moisture content of less than 1%, crush the freeze-dried sturgeon meat and sturgeon cartilage into 4000 mesh sturgeon superfine powder;
[0035] b. Mill the ultrafine sturgeon powder into 200 nm nano sturgeon powder;
[0036] c. Add 0.02M phosphate buffer with pH 7~8 and complex enzyme to the sturgeon nanopowder, and enzymatically hydrolyze for 48 h; the mass ratio of the sturgeon nanopowder to the phosphate buffer is 1:10, the complex enzyme The addition amount is 10 vitality units: 1 gram of sturgeon nanopowder, and the composite enzyme is a mixture of subtilisin, papain, and alkaline protease in a ratio of 3:3:4 to the vitality unit;
[0037] d. Centrifuge the supernatant, use an ultrafiltration membrane separator with a molecular weight cut-off of less than 10000 Da to separate, collect the liquid passing through the ultrafiltration membrane, pass the collected liquid throug...
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